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differentiation 86  (Affinity Biosciences)

 
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    Affinity Biosciences differentiation 86
    Differentiation 86, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/differentiation+86/31+anti+differentiation/pm41720005-72-5-38
    Average 86 stars, based on 1 article reviews
    differentiation 86 - by Bioz Stars, 2026-11
    86/100 stars

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    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of <t>CD86</t> in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.
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    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of <t>CD86</t> in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.
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    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of <t>CD86</t> in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.
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    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of <t>CD86</t> in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.
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    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of <t>CD86</t> in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.
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    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of <t>CD86</t> in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.
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    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of <t>CD86</t> in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.
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    Image Search Results


    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of CD86 in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.

    Journal: Journal of Advanced Research

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling

    doi: 10.1016/j.jare.2025.07.017

    Figure Lengend Snippet: HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of CD86 in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.

    Article Snippet: Tissue sections of each group were blocked with 1 % bovine serum albumin and 10 % donkey serum at room temperature for 1 h and then incubated at 4 °C overnight with primary antibodies for neuronal nuclear protein (NeuN) (1:500, Abcam, ab104224), ionized calcium-binding adapter molecule 1 (IBA-1) (1:500, Abcam, ab5076), glial fibrillary acidic protein (GFAP) (1:500, Millipore, MAB360), FXR (1:200, Proteintech, 25055–1-AP), MMP-2 (1:200, Proteintech, 10373-2-AP), MMP-9 (1:200, Proteintech, 10375-2-AP), PPAR-γ (1:200, Proteintech, 16643–1-AP), NLRP3 (1:200, Immunoway, YT5382), and cluster of differentiation 86 (CD86) (1:200, CST, #91882).

    Techniques: Immunofluorescence, Staining, Fluorescence, Expressing, Western Blot