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anti dgat1 antibody  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti dgat1 antibody
    Anti Dgat1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dgat1/DGAT1+Antibody+-+BSA+Free/10__1038_slash_s41420___026___03272___y-94-8-11
    Average 94 stars, based on 31 article reviews
    anti dgat1 antibody - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: One Week of Overeating Upregulates Angiogenic and Lipolytic Gene Expression in Human Subcutaneous Adipose Tissue from Exercise Trained and Untrained Adults
    Article Snippet: Following lysis and solubilization, protein concentration was measured by bicinchoninic acid assay (ThermoFisher cat 23225) and 15-20ug protein was used to measure protein abundance by SDS-page. .. Primary antibodies (incubated overnight) were: pERK thr202/tyr304 (Cell Signaling Technology cat 4376), ERK (Cell Signaling Technology cat 4376), pHSL ser660 (Cell Signaling Technology cat 4126), HSL (Cell Signaling Technology cat 18381), pATGL ser406 (gifted in kind from Dr. Matthew Watt, University of Melbourne), ATGL (Cell Signaling Technology cat 2138), GPAT1 (Invitrogen cat PA5-20524), DGAT1 (Novus Biologicals cat NB110-41487), DGAT2 (Santa Cruz cat sc-66859), CGI-58 (Abcam cat ab183739). .. Nitrocellulose membranes were stained after transfer with Memcode Reversible Protein Stain (ThermoScientific cat 24580) to quantify total protein per lane.

    Article Title: Very low-carbohydrate diet with higher protein ratio improves lipid metabolism and inflammation in rats with diet-induced nonalcoholic fatty liver disease.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats.
    Article Snippet: .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight. .. The membranes were washed three times with TBS-T for about 10 min each and then incubated with secondary anti-rabbit IgG (Santa Cruz Biotechnology Inc. Dallas, TX, U.S.A., sc-2004, 1:4000) antibody at room temperature for 40 min. After incubating with the secondary antibody, the membranes were three times washed with TBS-T for about 10 min each.

    Article Title: Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats
    Article Snippet: Protein aliquots (each 50 μg/well) were separated on an 8–12% sodium dodecyl sulphate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (GE Healthcare Life Sciences, Pittsburgh, PA, USA). .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight. .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight.

    Cell-Signaling:

    Article Title: One Week of Overeating Upregulates Angiogenic and Lipolytic Gene Expression in Human Subcutaneous Adipose Tissue from Exercise Trained and Untrained Adults
    Article Snippet: Following lysis and solubilization, protein concentration was measured by bicinchoninic acid assay (ThermoFisher cat 23225) and 15-20ug protein was used to measure protein abundance by SDS-page. .. Primary antibodies (incubated overnight) were: pERK thr202/tyr304 (Cell Signaling Technology cat 4376), ERK (Cell Signaling Technology cat 4376), pHSL ser660 (Cell Signaling Technology cat 4126), HSL (Cell Signaling Technology cat 18381), pATGL ser406 (gifted in kind from Dr. Matthew Watt, University of Melbourne), ATGL (Cell Signaling Technology cat 2138), GPAT1 (Invitrogen cat PA5-20524), DGAT1 (Novus Biologicals cat NB110-41487), DGAT2 (Santa Cruz cat sc-66859), CGI-58 (Abcam cat ab183739). .. Nitrocellulose membranes were stained after transfer with Memcode Reversible Protein Stain (ThermoScientific cat 24580) to quantify total protein per lane.

    Blocking Assay:

    Article Title: Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats.
    Article Snippet: .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight. .. The membranes were washed three times with TBS-T for about 10 min each and then incubated with secondary anti-rabbit IgG (Santa Cruz Biotechnology Inc. Dallas, TX, U.S.A., sc-2004, 1:4000) antibody at room temperature for 40 min. After incubating with the secondary antibody, the membranes were three times washed with TBS-T for about 10 min each.

    Article Title: Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats
    Article Snippet: Protein aliquots (each 50 μg/well) were separated on an 8–12% sodium dodecyl sulphate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (GE Healthcare Life Sciences, Pittsburgh, PA, USA). .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight. .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight.

    Saline:

    Article Title: Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats.
    Article Snippet: .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight. .. The membranes were washed three times with TBS-T for about 10 min each and then incubated with secondary anti-rabbit IgG (Santa Cruz Biotechnology Inc. Dallas, TX, U.S.A., sc-2004, 1:4000) antibody at room temperature for 40 min. After incubating with the secondary antibody, the membranes were three times washed with TBS-T for about 10 min each.

    Article Title: Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats
    Article Snippet: Protein aliquots (each 50 μg/well) were separated on an 8–12% sodium dodecyl sulphate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (GE Healthcare Life Sciences, Pittsburgh, PA, USA). .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight. .. After blocking, the membranes were incubated with primary antibodies against GAPDH (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-32233, 1:2000), DGAT1 (Novus Biologicals, Littleton, CO, USA, NB110-41487, 1:1000), and DGAT2 (Novus Biologicals, Littleton, CO, USA, NBP1-71701, 1:1000) diluted in Tris-buffered saline-Tween 20 (TBS-T) at 4 °C overnight.



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    Image Search Results


    TAG are synthesized in the ER through sequential enzymatic reactions of GPAT, LPAAT, PAP1, and DGAT. CE are generated by SOAT. Seipin facilitates the incorporation of neutral lipids into the growing LD core, which buds from the ER and become coated by perilipins (PLINs). Stored neutral lipids are mobilized through lipolysis mediated by ATGL, HSL, and MGL or degraded via lysosomal lipophagy. Released free fatty acids and cholesterol can be utilized for cellular metabolism, while cholesterol may also undergo ABCA1- and ABCG1-mediated efflux to ApoE-containing HDL-like particles. Alternatively, cholesterol can be converted by CH25H to 25-hydroxycholesterol (25-HC), which readily crosses cellular membranes for export.

    Journal: bioRxiv

    Article Title: Accumulation of Lipid Droplets in Microglia following Neonatal Brain Hypoxia-Ischemia

    doi: 10.64898/2026.07.18.739219

    Figure Lengend Snippet: TAG are synthesized in the ER through sequential enzymatic reactions of GPAT, LPAAT, PAP1, and DGAT. CE are generated by SOAT. Seipin facilitates the incorporation of neutral lipids into the growing LD core, which buds from the ER and become coated by perilipins (PLINs). Stored neutral lipids are mobilized through lipolysis mediated by ATGL, HSL, and MGL or degraded via lysosomal lipophagy. Released free fatty acids and cholesterol can be utilized for cellular metabolism, while cholesterol may also undergo ABCA1- and ABCG1-mediated efflux to ApoE-containing HDL-like particles. Alternatively, cholesterol can be converted by CH25H to 25-hydroxycholesterol (25-HC), which readily crosses cellular membranes for export.

    Article Snippet: DGAT1 inhibitor T863 (Cat# HY-32219, MedChemiExpress LCC, Monmouth Junction, NJ), and DGAT2 inhibitor PF-06424439 (Cat# HY-108341A, MCE) were dissolved in DMSO and used at 30 μM and 40 μM, respectively.

    Techniques: Synthesized, Generated

    A). Representative images of PLIN2 (white) expression and Dextran uptake (green) in BV2 cells following DGAT inhibitor treatment. BV2 cells were treated with DGAT1 inhibitor T863 or DGAT2 inhibitor PF-06424439 (PF) or DMSO vehicle control under normoxic control or OGD condition. Scale bar = 20 µm. n = 3 per group. B). Representative flow cytometric histograms showing PLIN2 fluorescence intensity curves under different treatment conditions. Blank group cells received medium as no-treatment control. C). Percentage of PLIN2 + cells in BV2 cells under different treatment conditions. D). PLIN2 mean fluorescence intensity (MFI) in BV2 cells under different treatment conditions. E). Representative flow cytometric histograms showing Dextran 40 fluorescence intensity curves under different conditions. F). Percentage of Dextran 40 + cells in BV2 cells under different treatment conditions. G). Dextran 40 MFI under different treatment conditions. For flow cytometry, n = 6 per group; data are presented as mean ± SD; two-way ANOVA (OGD × drug) with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001 vs. OGD and DMSO group; ### p < 0.001 vs. OGD + Blank group.

    Journal: bioRxiv

    Article Title: Accumulation of Lipid Droplets in Microglia following Neonatal Brain Hypoxia-Ischemia

    doi: 10.64898/2026.07.18.739219

    Figure Lengend Snippet: A). Representative images of PLIN2 (white) expression and Dextran uptake (green) in BV2 cells following DGAT inhibitor treatment. BV2 cells were treated with DGAT1 inhibitor T863 or DGAT2 inhibitor PF-06424439 (PF) or DMSO vehicle control under normoxic control or OGD condition. Scale bar = 20 µm. n = 3 per group. B). Representative flow cytometric histograms showing PLIN2 fluorescence intensity curves under different treatment conditions. Blank group cells received medium as no-treatment control. C). Percentage of PLIN2 + cells in BV2 cells under different treatment conditions. D). PLIN2 mean fluorescence intensity (MFI) in BV2 cells under different treatment conditions. E). Representative flow cytometric histograms showing Dextran 40 fluorescence intensity curves under different conditions. F). Percentage of Dextran 40 + cells in BV2 cells under different treatment conditions. G). Dextran 40 MFI under different treatment conditions. For flow cytometry, n = 6 per group; data are presented as mean ± SD; two-way ANOVA (OGD × drug) with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001 vs. OGD and DMSO group; ### p < 0.001 vs. OGD + Blank group.

    Article Snippet: DGAT1 inhibitor T863 (Cat# HY-32219, MedChemiExpress LCC, Monmouth Junction, NJ), and DGAT2 inhibitor PF-06424439 (Cat# HY-108341A, MCE) were dissolved in DMSO and used at 30 μM and 40 μM, respectively.

    Techniques: Expressing, Control, Fluorescence, Flow Cytometry

    A). Representative images of BODIPY (green) and IL-1β (white) expression in BV2 cells following DGAT inhibitor treatment. BV2 cells were treated with DGAT1 inhibitor T863 or DGAT2 inhibitor PF-06424439 (PF) or DMSO vehicle control under normoxic control or OGD condition. Scale bar = 20 µm. n = 3 per group. B). Representative flow cytometric histograms showing BODIPY fluorescence intensity curves in BV2 cells under different treatment conditions. Blank group cells received medium as no-treatment control. C). Percentage of BODIPY + cells in BV2 cells under different treatment conditions. D). BODIPY mean fluorescence intensity (MFI) under different treatment conditions. E). Representative flow cytometric histograms showing IL-1β fluorescence intensity curves under different treatment conditions. F). Percentage of IL-1β + cells in BV2 cells under different treatment conditions. G). IL-1β MFI in BV2 cells under different treatment conditions. For flow cytometry, n = 6 per group; data are presented as mean ± SD; two-way ANOVA (OGD × drug) with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001 vs. OGD and DMSO group; ### p < 0.001 vs. OGD + Blank.

    Journal: bioRxiv

    Article Title: Accumulation of Lipid Droplets in Microglia following Neonatal Brain Hypoxia-Ischemia

    doi: 10.64898/2026.07.18.739219

    Figure Lengend Snippet: A). Representative images of BODIPY (green) and IL-1β (white) expression in BV2 cells following DGAT inhibitor treatment. BV2 cells were treated with DGAT1 inhibitor T863 or DGAT2 inhibitor PF-06424439 (PF) or DMSO vehicle control under normoxic control or OGD condition. Scale bar = 20 µm. n = 3 per group. B). Representative flow cytometric histograms showing BODIPY fluorescence intensity curves in BV2 cells under different treatment conditions. Blank group cells received medium as no-treatment control. C). Percentage of BODIPY + cells in BV2 cells under different treatment conditions. D). BODIPY mean fluorescence intensity (MFI) under different treatment conditions. E). Representative flow cytometric histograms showing IL-1β fluorescence intensity curves under different treatment conditions. F). Percentage of IL-1β + cells in BV2 cells under different treatment conditions. G). IL-1β MFI in BV2 cells under different treatment conditions. For flow cytometry, n = 6 per group; data are presented as mean ± SD; two-way ANOVA (OGD × drug) with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001 vs. OGD and DMSO group; ### p < 0.001 vs. OGD + Blank.

    Article Snippet: DGAT1 inhibitor T863 (Cat# HY-32219, MedChemiExpress LCC, Monmouth Junction, NJ), and DGAT2 inhibitor PF-06424439 (Cat# HY-108341A, MCE) were dissolved in DMSO and used at 30 μM and 40 μM, respectively.

    Techniques: Expressing, Control, Fluorescence, Flow Cytometry