Journal: bioRxiv
Article Title: Ciliary ARLs drive renal cystogenesis
doi: 10.1101/2025.11.20.689124
Figure Lengend Snippet: Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.
Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).
Techniques: Staining, Western Blot, Marker, Control