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dendritic cell generation medium  (PromoCell)


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    PromoCell dendritic cell generation medium
    Dendritic Cell Generation Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dendritic+cell+generation+medium/DC+Generation+Medium/pmc12910075-493-5-9
    Average 93 stars, based on 12 article reviews
    dendritic cell generation medium - by Bioz Stars, 2026-10
    93/100 stars

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    Cell Culture:

    Article Title: Tmem110 regulates the conformation of TRPML1 to maintain endolysosomal homeostasis and prevent mitochondrial DNA leakage and pathological self-DNA processing
    Article Snippet: Human monocyte-derived CD14 + cells (hMoCD14 + , Cat. No. C-12909) were purchased from PromoCell (Germany). .. These cells were cultured in Dendritic Cell Generation Medium (PromoCell) and maintained at 37 °C with 5% CO2. ..

    Article Title: Tmem110 regulates the conformation of TRPML1 to maintain endolysosomal homeostasis and prevent mitochondrial DNA leakage and pathological self-DNA processing.
    Article Snippet: Human monocyte-derived CD14+ cells (hMoCD14+, Cat. No. C-12909) were purchased from PromoCell (Germany). .. These cells were cultured in Dendritic Cell Generation Medium (PromoCell) and maintained at 37°C with 5% CO2. ..

    Incubation:

    Article Title: Antigen presenting scaffolds for immune-cell manipulation
    Article Snippet: Briefly, PBMC's in PromoCell Monocyte Attachment Medium were plated out in tissue culture plates at a density of 2-3 million/cm2 for 1 hour at 5% CO2 and 37° C. Monocytes were capture by removing non-adherent cells. .. Differentiation into immature moDC (day 0) was started by adding PromoCell Dendritic Cell Generation Medium supplemented with 1× Component A of the Cytokine Pack moDC (supplied at 100×) and incubation for 3 days at 37° C. and 5% CO2. .. Medium change was performed on day 3 by aspirating the medium from the cells and adding fresh PromoCell DC Generation Medium supplemented with 1× Component A of the Cytokine Pack moDC to the cells.

    Article Title: Antigen presenting scaffolds for immune-cell manipulation
    Article Snippet: Briefly, PBMC's in PromoCell Monocyte Attachment Medium were plated out in tissue culture plates at a density of 2-3 million/cm2 for 1 hour at 5% CO2 and 37° C. Monocytes were capture by removing non-adherent cells. .. Differentiation into immature moDC (day 0) was started by adding PromoCell Dendritic Cell Generation Medium supplemented with 1× Component A of the Cytokine Pack moDC (supplied at 100×) and incubation for 3 days at 37° C. and 5% CO2. .. Medium change was performed on day 3 by aspirating the medium from the cells and adding fresh PromoCell DC Generation Medium supplemented with 1× Component A of the Cytokine Pack moDC to the cells.

    other:

    Article Title: Pleiotropic effects of a Camellia sinensis leaf extract on in vitro and in vivo skin health characteristics.
    Article Snippet: Int J Cosmet Sci.. 2025;00:1–16.. | 1wileyonlinelibrary.com/journal/ics © 2025 Society of Cosmetic Scientists and the Société Française de Cosmétologie.



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    Poly(I:C) improves the adjuvanticity of inactivated spores and YC-NaMA in human monocyte-derived dendritic cells. <t>(A)</t> <t>CD14+</t> monocytes were magnetically enriched from healthy donor PBMCs After 6 days of differentiation, <t>moDCs</t> were either left unstimulated, or treated with LPS, poly(I:C) or vaccine delivery systems with or without poly (I:C). After 48 hours, cells were surface stained for activation induced markers (MHC-I, MHC-II, CD80, CD86, CD40 and CCR7) for FACS analysis. Culture supernatants were also obtained to quantify secreted inflammatory cytokines (IL-1β, IFN-α2, IFNγ, TNFα, IL-6, IL-18, IL-10, IL-17A, IL-12p70, IL-23, IL-33, CCL2 and CXCL8). (B) Heatmap showing the log10 fold increase in cell-surface and secreted soluble mediators for each treatment group relative to unstimulated group. (C) Heatmap showing P values comparing YC-Nama or Spore with and without Poly(I:C). Data is obtained from the average of two healthy donors. Two-way ANOVA followed by Tukey’s multiple comparisons test was used to compare the groups. Asterisks depicted in A are significant differences from unstimulated. Asterisks in B show significant difference between two groups. P<0.05 = *; P<0.01 = **; P<0.0001 = ****.
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    Poly(I:C) improves the adjuvanticity of inactivated spores and YC-NaMA in human monocyte-derived dendritic cells. (A) CD14+ monocytes were magnetically enriched from healthy donor PBMCs After 6 days of differentiation, moDCs were either left unstimulated, or treated with LPS, poly(I:C) or vaccine delivery systems with or without poly (I:C). After 48 hours, cells were surface stained for activation induced markers (MHC-I, MHC-II, CD80, CD86, CD40 and CCR7) for FACS analysis. Culture supernatants were also obtained to quantify secreted inflammatory cytokines (IL-1β, IFN-α2, IFNγ, TNFα, IL-6, IL-18, IL-10, IL-17A, IL-12p70, IL-23, IL-33, CCL2 and CXCL8). (B) Heatmap showing the log10 fold increase in cell-surface and secreted soluble mediators for each treatment group relative to unstimulated group. (C) Heatmap showing P values comparing YC-Nama or Spore with and without Poly(I:C). Data is obtained from the average of two healthy donors. Two-way ANOVA followed by Tukey’s multiple comparisons test was used to compare the groups. Asterisks depicted in A are significant differences from unstimulated. Asterisks in B show significant difference between two groups. P<0.05 = *; P<0.01 = **; P<0.0001 = ****.

    Journal: Frontiers in Immunology

    Article Title: Mucosal and systemic immune responses after a single intranasal dose of nanoparticle and spore-based subunit vaccines in mice with pre-existing lung mycobacterial immunity

    doi: 10.3389/fimmu.2023.1306449

    Figure Lengend Snippet: Poly(I:C) improves the adjuvanticity of inactivated spores and YC-NaMA in human monocyte-derived dendritic cells. (A) CD14+ monocytes were magnetically enriched from healthy donor PBMCs After 6 days of differentiation, moDCs were either left unstimulated, or treated with LPS, poly(I:C) or vaccine delivery systems with or without poly (I:C). After 48 hours, cells were surface stained for activation induced markers (MHC-I, MHC-II, CD80, CD86, CD40 and CCR7) for FACS analysis. Culture supernatants were also obtained to quantify secreted inflammatory cytokines (IL-1β, IFN-α2, IFNγ, TNFα, IL-6, IL-18, IL-10, IL-17A, IL-12p70, IL-23, IL-33, CCL2 and CXCL8). (B) Heatmap showing the log10 fold increase in cell-surface and secreted soluble mediators for each treatment group relative to unstimulated group. (C) Heatmap showing P values comparing YC-Nama or Spore with and without Poly(I:C). Data is obtained from the average of two healthy donors. Two-way ANOVA followed by Tukey’s multiple comparisons test was used to compare the groups. Asterisks depicted in A are significant differences from unstimulated. Asterisks in B show significant difference between two groups. P<0.05 = *; P<0.01 = **; P<0.0001 = ****.

    Article Snippet: To differentiate CD14+ monocytes to immature moDCs, we used a commercially available dendritic cell generation medium (PromoCell ® , C-28050).

    Techniques: Derivative Assay, Staining, Activation Assay