Review



area quantification and deconvolution modules of halo image analysis platform  (Indica Labs)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Indica Labs area quantification and deconvolution modules of halo image analysis platform
    Area Quantification And Deconvolution Modules Of Halo Image Analysis Platform, supplied by Indica Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/area+quantification+and+deconvolution+modules+of+halo+image+analysis+platform/pmc09387135-316-13-23
    Average 90 stars, based on 1 article reviews
    area quantification and deconvolution modules of halo image analysis platform - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Retinoic acid signaling regulates spatiotemporal specification of human green and red cones.
    Article Snippet: PLOS Biology | https://doi.org/10.1371/journal.pbio.3002464 January 11, 2024 16 / 28 To visualize the nuclear counterstain alongside the Basescope chromogenic stain, we used the Indica Labs–Deconvolution v1.1.7 module of the HALO Image Analysis Platform version 3.5.3577 (Indica Labs) to separate the chromogenic stains of the RGB images.

    Immunohistochemistry:

    Article Title: Cancer-associated fibroblast-derived Dickkopf-1 suppresses NK cell cytotoxicity in breast cancer
    Article Snippet: Next, slide images were scanned via a Zeiss AxioScan 7 microscope. .. All IHC analyses were performed on the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). ..

    Article Title: Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancer
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs) as appropriate, scanned via a Zeiss AxioScan 7 microscope and IHC analyses performed using the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). .. The following antibodies were used: αSMA (Abcam, Cat# ab5694, 1:1500 (Human), 1:200 (Mouse)), COL1a1 (Cell signaling, Cat# 72026, 1:100 (Mouse)), COL14a1 (Cell signaling, Cat# 61964, 1:200 (Human)), DKK1 (Proteintech, Cat# 21112-1-AP, 1:100 (Human), 1:3000 (Mouse)), PDGFRα (Cell signaling, Cat# 5241, 1:200 (Human)), PanCK (Novus, Cat# NBP2-29429, 1:1000 (Human)).

    Article Title: Myofibroblastic CAFs arising from bone-resident osteoblast precursors retain an osteolineage signature and support breast cancer progression via Osterix-mediated signaling
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs) as appropriate, scanned via a Zeiss AxioScan 7 microscope and IHC analyses performed using the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). .. The puromycin-resistant pLenti-puro ORF clone of Sp7 (mGFP-tagged – Origene MR226379L4) was used to ectopically express Sp7 ( Osx ) in MMFs or immortalized CAFs. pLenti-puro (Addgene plasmid #39481) was used as a vehicle control.

    Article Title: Bone-derived Osterix+ osteolineage cells are a source of tumor-promoting myofibroblastic cancer-associated fibroblasts in breast cancer.
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs), as appropriate, scanned with a Zeiss AxioScan 7 microscope, and IHC analyses performed using the HALO image analysis platform (Indica Labs; Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). ..

    Multiplex Assay:

    Article Title: Cancer-associated fibroblast-derived Dickkopf-1 suppresses NK cell cytotoxicity in breast cancer
    Article Snippet: Next, slide images were scanned via a Zeiss AxioScan 7 microscope. .. All IHC analyses were performed on the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). ..

    Article Title: Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancer
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs) as appropriate, scanned via a Zeiss AxioScan 7 microscope and IHC analyses performed using the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). .. The following antibodies were used: αSMA (Abcam, Cat# ab5694, 1:1500 (Human), 1:200 (Mouse)), COL1a1 (Cell signaling, Cat# 72026, 1:100 (Mouse)), COL14a1 (Cell signaling, Cat# 61964, 1:200 (Human)), DKK1 (Proteintech, Cat# 21112-1-AP, 1:100 (Human), 1:3000 (Mouse)), PDGFRα (Cell signaling, Cat# 5241, 1:200 (Human)), PanCK (Novus, Cat# NBP2-29429, 1:1000 (Human)).

    Article Title: Myofibroblastic CAFs arising from bone-resident osteoblast precursors retain an osteolineage signature and support breast cancer progression via Osterix-mediated signaling
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs) as appropriate, scanned via a Zeiss AxioScan 7 microscope and IHC analyses performed using the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). .. The puromycin-resistant pLenti-puro ORF clone of Sp7 (mGFP-tagged – Origene MR226379L4) was used to ectopically express Sp7 ( Osx ) in MMFs or immortalized CAFs. pLenti-puro (Addgene plasmid #39481) was used as a vehicle control.

    Article Title: Bone-derived Osterix+ osteolineage cells are a source of tumor-promoting myofibroblastic cancer-associated fibroblasts in breast cancer.
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs), as appropriate, scanned with a Zeiss AxioScan 7 microscope, and IHC analyses performed using the HALO image analysis platform (Indica Labs; Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). ..

    Microscopy:

    Article Title: Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancer
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs) as appropriate, scanned via a Zeiss AxioScan 7 microscope and IHC analyses performed using the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). .. The following antibodies were used: αSMA (Abcam, Cat# ab5694, 1:1500 (Human), 1:200 (Mouse)), COL1a1 (Cell signaling, Cat# 72026, 1:100 (Mouse)), COL14a1 (Cell signaling, Cat# 61964, 1:200 (Human)), DKK1 (Proteintech, Cat# 21112-1-AP, 1:100 (Human), 1:3000 (Mouse)), PDGFRα (Cell signaling, Cat# 5241, 1:200 (Human)), PanCK (Novus, Cat# NBP2-29429, 1:1000 (Human)).

    Article Title: Myofibroblastic CAFs arising from bone-resident osteoblast precursors retain an osteolineage signature and support breast cancer progression via Osterix-mediated signaling
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs) as appropriate, scanned via a Zeiss AxioScan 7 microscope and IHC analyses performed using the HALO image analysis platform (Indica Labs, Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). .. The puromycin-resistant pLenti-puro ORF clone of Sp7 (mGFP-tagged – Origene MR226379L4) was used to ectopically express Sp7 ( Osx ) in MMFs or immortalized CAFs. pLenti-puro (Addgene plasmid #39481) was used as a vehicle control.

    Article Title: Bone-derived Osterix+ osteolineage cells are a source of tumor-promoting myofibroblastic cancer-associated fibroblasts in breast cancer.
    Article Snippet: Immunostaining was chromogenically visualized using the Bond Polymer Refine Detection (#DS9800, Leica Biosystems) or the Bond Polymer Refine Red Detection (#DS9390, Leica Biosystems). .. Slides were mounted using Xylene-based Cytoseal (Thermo Fisher) or Vectamount (Vector Labs), as appropriate, scanned with a Zeiss AxioScan 7 microscope, and IHC analyses performed using the HALO image analysis platform (Indica Labs; Deconvolution v1.1.1, Multiplex IHC v.3.2.3 algorithms). ..



    Similar Products

    deconvolution analysis  (Olympus)
    99
    Olympus deconvolution analysis
    Triple co-localization of β-catenin, NDRG1, and PKCα demonstrates the formation of a possible metabolon, decreasing β-catenin levels and nuclear localization. A – H , NDRG1 overexpression in PANC-1 cells significantly increases the co-localization between β-catenin, NDRG1, and PKCα. A and B , VC and NDRG1 overexpressing PANC-1 cells were incubated for 24 h/37 °C in the presence and absence of WNT3a (100 ng/ml). The cells were then examined for β-catenin ( green ), NDRG1 ( red ), and PKCα ( blue ) expression and triple co-localization ( white ) using confocal immunofluorescence microscopy. Studies were performed using a 100× objective at the same acquisition setting with Olympus Fluoview FV3000 software. Images were digitally magnified for better demonstration of the possible co-localization. The scale bar = 3 μm for all images except for the inset images, where the scale bar = 9 μm. C and D , <t>deconvolution</t> analysis was then implemented on the images in ( A and B ) using Olympus CellSens imaging software. White arrows demonstrate triple co-localization and possible formation of the metabolon. The scale bar = 3 μm for all deconvolution images except for the inset images, where the scale bar = 1.8 μm. In all studies, the images are representative of three experiments, and the quantitative analysis of the pixel intensities is shown for ( E ) β-catenin; ( F ) NDRG1; ( G ) PKCα; and ( H ) Co-localization between β-catenin, NDRG1, and PKCα. Quantitative analyses were performed using ImageJ software and were presented as the mean ± SD ( n = 3). Analysis of pixel intensity and co-localization were performed using 24 cells. Statistical significance is denoted as ∗∗ p < 0.01 and ∗∗∗ p < 0.001 comparing NDRG1 overexpressing PANC-1 cells in the presence and absence of WNT3a to VC cells in the absence of WNT3a; or ### p < 0.001 comparing NDRG1 overexpressing PANC-1 cells to VC cells in the presence of WNT3a.
    Deconvolution Analysis, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/cellSens+Imaging+Software/pmc11261793-400-20-25
    Average 99 stars, based on 1 article reviews
    deconvolution analysis - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    deconvolution algorithm deltavision softworx image analysis program  (Softworx Inc)
    90
    Softworx Inc deconvolution algorithm deltavision softworx image analysis program
    Triple co-localization of β-catenin, NDRG1, and PKCα demonstrates the formation of a possible metabolon, decreasing β-catenin levels and nuclear localization. A – H , NDRG1 overexpression in PANC-1 cells significantly increases the co-localization between β-catenin, NDRG1, and PKCα. A and B , VC and NDRG1 overexpressing PANC-1 cells were incubated for 24 h/37 °C in the presence and absence of WNT3a (100 ng/ml). The cells were then examined for β-catenin ( green ), NDRG1 ( red ), and PKCα ( blue ) expression and triple co-localization ( white ) using confocal immunofluorescence microscopy. Studies were performed using a 100× objective at the same acquisition setting with Olympus Fluoview FV3000 software. Images were digitally magnified for better demonstration of the possible co-localization. The scale bar = 3 μm for all images except for the inset images, where the scale bar = 9 μm. C and D , <t>deconvolution</t> analysis was then implemented on the images in ( A and B ) using Olympus CellSens imaging software. White arrows demonstrate triple co-localization and possible formation of the metabolon. The scale bar = 3 μm for all deconvolution images except for the inset images, where the scale bar = 1.8 μm. In all studies, the images are representative of three experiments, and the quantitative analysis of the pixel intensities is shown for ( E ) β-catenin; ( F ) NDRG1; ( G ) PKCα; and ( H ) Co-localization between β-catenin, NDRG1, and PKCα. Quantitative analyses were performed using ImageJ software and were presented as the mean ± SD ( n = 3). Analysis of pixel intensity and co-localization were performed using 24 cells. Statistical significance is denoted as ∗∗ p < 0.01 and ∗∗∗ p < 0.001 comparing NDRG1 overexpressing PANC-1 cells in the presence and absence of WNT3a to VC cells in the absence of WNT3a; or ### p < 0.001 comparing NDRG1 overexpressing PANC-1 cells to VC cells in the presence of WNT3a.
    Deconvolution Algorithm Deltavision Softworx Image Analysis Program, supplied by Softworx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/deconvolution+algorithm+deltavision+softworx+image+analysis+program/pm37667030-186-6-9
    Average 90 stars, based on 1 article reviews
    deconvolution algorithm deltavision softworx image analysis program - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    area quantification and deconvolution modules of halo image analysis platform  (Indica Labs)
    90
    Indica Labs area quantification and deconvolution modules of halo image analysis platform
    Triple co-localization of β-catenin, NDRG1, and PKCα demonstrates the formation of a possible metabolon, decreasing β-catenin levels and nuclear localization. A – H , NDRG1 overexpression in PANC-1 cells significantly increases the co-localization between β-catenin, NDRG1, and PKCα. A and B , VC and NDRG1 overexpressing PANC-1 cells were incubated for 24 h/37 °C in the presence and absence of WNT3a (100 ng/ml). The cells were then examined for β-catenin ( green ), NDRG1 ( red ), and PKCα ( blue ) expression and triple co-localization ( white ) using confocal immunofluorescence microscopy. Studies were performed using a 100× objective at the same acquisition setting with Olympus Fluoview FV3000 software. Images were digitally magnified for better demonstration of the possible co-localization. The scale bar = 3 μm for all images except for the inset images, where the scale bar = 9 μm. C and D , <t>deconvolution</t> analysis was then implemented on the images in ( A and B ) using Olympus CellSens imaging software. White arrows demonstrate triple co-localization and possible formation of the metabolon. The scale bar = 3 μm for all deconvolution images except for the inset images, where the scale bar = 1.8 μm. In all studies, the images are representative of three experiments, and the quantitative analysis of the pixel intensities is shown for ( E ) β-catenin; ( F ) NDRG1; ( G ) PKCα; and ( H ) Co-localization between β-catenin, NDRG1, and PKCα. Quantitative analyses were performed using ImageJ software and were presented as the mean ± SD ( n = 3). Analysis of pixel intensity and co-localization were performed using 24 cells. Statistical significance is denoted as ∗∗ p < 0.01 and ∗∗∗ p < 0.001 comparing NDRG1 overexpressing PANC-1 cells in the presence and absence of WNT3a to VC cells in the absence of WNT3a; or ### p < 0.001 comparing NDRG1 overexpressing PANC-1 cells to VC cells in the presence of WNT3a.
    Area Quantification And Deconvolution Modules Of Halo Image Analysis Platform, supplied by Indica Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/area+quantification+and+deconvolution+modules+of+halo+image+analysis+platform/pmc09387135-316-13-23
    Average 90 stars, based on 1 article reviews
    area quantification and deconvolution modules of halo image analysis platform - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    automated image analysis algorithm color deconvolution  (Aperio Technologies)
    90
    Aperio Technologies automated image analysis algorithm color deconvolution
    Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color <t>deconvolution</t> (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.
    Automated Image Analysis Algorithm Color Deconvolution, supplied by Aperio Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/automated+image+analysis+algorithm+color+deconvolution/pmc08750937-68-18-27
    Average 90 stars, based on 1 article reviews
    automated image analysis algorithm color deconvolution - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    softworx suite r6.1.1 image analysis deconvolution software  (Applied Precision Inc)
    90
    Applied Precision Inc softworx suite r6.1.1 image analysis deconvolution software
    Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color <t>deconvolution</t> (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.
    Softworx Suite R6.1.1 Image Analysis Deconvolution Software, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/softworx+suite+r6+1+1+image+analysis+deconvolution+software/pmc08318279-409-32-39
    Average 90 stars, based on 1 article reviews
    softworx suite r6.1.1 image analysis deconvolution software - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    deconvolution imaging analysis system  (VAYTEK INC)
    90
    VAYTEK INC deconvolution imaging analysis system
    Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color <t>deconvolution</t> (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.
    Deconvolution Imaging Analysis System, supplied by VAYTEK INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/deconvolution+imaging+analysis+system/pmc07996795-209-8-12
    Average 90 stars, based on 1 article reviews
    deconvolution imaging analysis system - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    9.3—3d/4d image visualization analysis deconvolution  (Oxford Instruments)
    90
    Oxford Instruments 9.3—3d/4d image visualization analysis deconvolution
    Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color <t>deconvolution</t> (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.
    9.3—3d/4d Image Visualization Analysis Deconvolution, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+image+analysis/10__1109_slash_access__2020__3040319-948-7-12
    Average 90 stars, based on 1 article reviews
    9.3—3d/4d image visualization analysis deconvolution - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Triple co-localization of β-catenin, NDRG1, and PKCα demonstrates the formation of a possible metabolon, decreasing β-catenin levels and nuclear localization. A – H , NDRG1 overexpression in PANC-1 cells significantly increases the co-localization between β-catenin, NDRG1, and PKCα. A and B , VC and NDRG1 overexpressing PANC-1 cells were incubated for 24 h/37 °C in the presence and absence of WNT3a (100 ng/ml). The cells were then examined for β-catenin ( green ), NDRG1 ( red ), and PKCα ( blue ) expression and triple co-localization ( white ) using confocal immunofluorescence microscopy. Studies were performed using a 100× objective at the same acquisition setting with Olympus Fluoview FV3000 software. Images were digitally magnified for better demonstration of the possible co-localization. The scale bar = 3 μm for all images except for the inset images, where the scale bar = 9 μm. C and D , deconvolution analysis was then implemented on the images in ( A and B ) using Olympus CellSens imaging software. White arrows demonstrate triple co-localization and possible formation of the metabolon. The scale bar = 3 μm for all deconvolution images except for the inset images, where the scale bar = 1.8 μm. In all studies, the images are representative of three experiments, and the quantitative analysis of the pixel intensities is shown for ( E ) β-catenin; ( F ) NDRG1; ( G ) PKCα; and ( H ) Co-localization between β-catenin, NDRG1, and PKCα. Quantitative analyses were performed using ImageJ software and were presented as the mean ± SD ( n = 3). Analysis of pixel intensity and co-localization were performed using 24 cells. Statistical significance is denoted as ∗∗ p < 0.01 and ∗∗∗ p < 0.001 comparing NDRG1 overexpressing PANC-1 cells in the presence and absence of WNT3a to VC cells in the absence of WNT3a; or ### p < 0.001 comparing NDRG1 overexpressing PANC-1 cells to VC cells in the presence of WNT3a.

    Journal: The Journal of Biological Chemistry

    Article Title: Multi-modal mechanisms of the metastasis suppressor, NDRG1: Inhibition of WNT/β-catenin signaling by stabilization of protein kinase Cα

    doi: 10.1016/j.jbc.2024.107417

    Figure Lengend Snippet: Triple co-localization of β-catenin, NDRG1, and PKCα demonstrates the formation of a possible metabolon, decreasing β-catenin levels and nuclear localization. A – H , NDRG1 overexpression in PANC-1 cells significantly increases the co-localization between β-catenin, NDRG1, and PKCα. A and B , VC and NDRG1 overexpressing PANC-1 cells were incubated for 24 h/37 °C in the presence and absence of WNT3a (100 ng/ml). The cells were then examined for β-catenin ( green ), NDRG1 ( red ), and PKCα ( blue ) expression and triple co-localization ( white ) using confocal immunofluorescence microscopy. Studies were performed using a 100× objective at the same acquisition setting with Olympus Fluoview FV3000 software. Images were digitally magnified for better demonstration of the possible co-localization. The scale bar = 3 μm for all images except for the inset images, where the scale bar = 9 μm. C and D , deconvolution analysis was then implemented on the images in ( A and B ) using Olympus CellSens imaging software. White arrows demonstrate triple co-localization and possible formation of the metabolon. The scale bar = 3 μm for all deconvolution images except for the inset images, where the scale bar = 1.8 μm. In all studies, the images are representative of three experiments, and the quantitative analysis of the pixel intensities is shown for ( E ) β-catenin; ( F ) NDRG1; ( G ) PKCα; and ( H ) Co-localization between β-catenin, NDRG1, and PKCα. Quantitative analyses were performed using ImageJ software and were presented as the mean ± SD ( n = 3). Analysis of pixel intensity and co-localization were performed using 24 cells. Statistical significance is denoted as ∗∗ p < 0.01 and ∗∗∗ p < 0.001 comparing NDRG1 overexpressing PANC-1 cells in the presence and absence of WNT3a to VC cells in the absence of WNT3a; or ### p < 0.001 comparing NDRG1 overexpressing PANC-1 cells to VC cells in the presence of WNT3a.

    Article Snippet: The images of visualized cells were then examined using Olympus Fluoview software, and in some studies, images were processed for deconvolution analysis with CellSens software (Olympus) to improve contrast and image resolution.

    Techniques: Over Expression, Incubation, Expressing, Immunofluorescence, Microscopy, Software, Imaging

    Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color deconvolution (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.

    Journal: Cancers

    Article Title: Lysyl-Oxidase Dependent Extracellular Matrix Stiffness in Hodgkin Lymphomas: Mechanical and Topographical Evidence

    doi: 10.3390/cancers14010259

    Figure Lengend Snippet: Digital pathology imaging and measurement of lymph node stroma in HL and NHL. Slides of hematoxilin-eosin (HE, representative cases in Panel ( A )) or Mallory trichrome staining (representative cases in Panel ( B )) from LN sections of patients with HL ( n = 11), FL1-2 ( n = 6), FL3A ( n = 8), DLBCL ( n = 6), compared with 9 LDN, were subjected to Digital Pathology Imaging. Panel ( C ). HE sections were analyzed with the Genie software (right images in Panel ( A )) and the percentage area of stromal compartment per slide was calculated, adjusted to total tissue area in the image analyzed. Data are expressed as a percentage of green pseudocolor (stromal) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.05 vs. LDN, FL1-2 and FL3A. Panel ( D ): digital images of Mallory trichrome stained sections were analyzed with an automated image analysis algorithm for color deconvolution (right images in Panel ( B )) and collagen areas quantified by the Image Scope software. Data are expressed as percentage of blue (collagen) areas and are the mean ± SEM of the indicated number of cases for each histological type. * p < 0.005 vs. LDN, FL1-2 and FL3A.

    Article Snippet: Layers of each virtual slide annotation were created manually, selecting the whole area, and analyzed with the automated image analysis algorithm color deconvolution (Aperio software version 9.1, Aperio Technologies, Nussloch GmbH, Wetzlar, Germany).

    Techniques: Imaging, Staining, Software