rabbit anti ddx6 antibody (Novus Biologicals)
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Novus Biologicals
rabbit anti ddx6 antibody
Rabbit Anti Ddx6 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddx6/DDX6+Antibody/us12565650-788-39-42
Average 94 stars, based on 19 article reviews
Rabbit Anti Ddx6 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddx6/DDX6+Antibody/us12565650-788-39-42
Average 94 stars, based on 19 article reviews
rabbit anti ddx6 antibody - by Bioz Stars,
2026-09
94/100 stars
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Biomarker Discovery:Article Title: Translational repression by 4E-T is crucial to maintain the prophase-I arrest in vertebrate oocytes Article Snippet: DDX6 (Abcam ab174277), DDB1 (Abcam ab109027) and 4E-T (Thermo Fisher Scientific PA5-51680) antibody validation can be found on the company website and they were previously used in Cheng et al., Science 378, (2022). .. CPEB1 (Biozol MBS9213514), ppMAPK (Cell Signaling #9106), MAPK (Santa Cruz sc-154), Cdk1 (Santa Cruz sc-54), p150 (BD Transduction Laboratories 610473), other:Article Title: Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b Article Snippet: The following antibodies were used: DCP1a (ab47811; Abcam), DCP1b (mAb #13233; Cell Signaling Technologies), EDC4 (ab72408; Abcam), DCP2 (ab28658; Abcam), EDC3 (mAb #14495; Cell Signaling Technologies), GAPDH (mAb #5174; Cell Signaling Technologies), Article Title: Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b Article Snippet: DCP1a (Abcam, Cambridge, UK, ab47811), DCP1b (Cell Signaling Technologies, Danvers, Massachusetts, USA, mAb #13233), EDC4 (Abcam, Cambridge, UK, ab72408), EDC3 (Cell Signaling Technologies, Danvers, Massachusetts, USA, mAb #14495), GAPDH (Cell Signaling Technologies, Danvers, Massachusetts, USA, mAb #5174), Article Title: Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b. Article Snippet: The following antibodies were used: DCP1a (ab47811; Abcam), DCP1b (mAb #13233; Cell Signaling Technologies), EDC4 (ab72408; Abcam), DCP2 (ab28658; Abcam), EDC3 (mAb #14495; Cell Signaling Technologies), GAPDH (mAb #5174; Cell Signaling Technologies), Blocking Assay:Article Title: MYCN-induced nucleolar stress drives an early senescence-like transcriptional program in hTERT-immortalized RPE cells. Article Snippet: Subsequently, cells were permeabilized with 0.3% Triton X-100 (8 min) and washed (2 × 5 min), after which a blocking step was added with 50% FBS for 30 min and incubated with primary antibody diluted in 50% FBS for 60 min. After minimally 3 washing steps with PBS, plates were incubated with secondary antibody diluted in 50% FBS for 60 min, washed again, and counterstained with DAPI (5 μg/ml) for 20 min. Primary antibodies were directed against p21 (F-5) (SC-6246, Santa Cruz Biotechnology Inc., 1:100 dilution), p53 (7F5) (#2527 T, Cell Signalling Technology, 1:1600 dilution), Lamin B1 (ab16048, Abcam, 1:1000 dilution), Ki-67 (M7240) (M724029-2, Agilent, 1:400 dilution), γH2AX (phospho-S139) (ab2893, Abcam, 1:1000 dilution), RPA32/RPA2 (9H8) (ab2175, Abcam, 1:300 dilution), Fibrillarin (H-140) (SC-25397, Santa Cruz Biotechnology Inc., 1:50 dilution), DDX6 (200–192, Novus, 1:300 dilution) and c a Fibrillarin 24 h 48 h 72 h * * MYCN-ONMYCN-OFF FIBRILLARIN DAPI MERGED M Y C N -O F F M Y C N -O N b 5 10 15 0 10 20 30 N uc le ol ar A re a S in gl e N uc le ol us F ra ct io n MYCN-OFF MYCN-ON 24 h 48 h 72 h *n. s. n. s. n. s. d 10 20 30 40 24 h C el ls w ith 1 n uc le ol us ( % ) 10 20 30 40 48 h 10 20 30 40 72 h MYCN-OFF MYCN-ON G1 S G2 G1 S G2 G1 S G2 *n. s. * * ** ** n. s. *n. s. Fibrillarin/DAPI Figure 6. .. Subsequently, cells were permeabilized with 0.3% Triton X-100 (8 min) and washed (2 × 5 min), after which a blocking step was added with 50% FBS for 30 min and incubated with primary antibody diluted in 50% FBS for 60 min. After minimally 3 washing steps with PBS, plates were incubated with secondary antibody diluted in 50% FBS for 60 min, washed again, and counterstained with DAPI (5 μg/ml) for 20 min. Primary antibodies were directed against p21 (F-5) (SC-6246, Santa Cruz Biotechnology Inc., 1:100 dilution), p53 (7F5) (#2527 T, Cell Signalling Technology, 1:1600 dilution), Lamin B1 (ab16048, Abcam, 1:1000 dilution), Ki-67 (M7240) (M724029-2, Agilent, 1:400 dilution), γH2AX (phospho-S139) (ab2893, Abcam, 1:1000 dilution), RPA32/RPA2 (9H8) (ab2175, Abcam, 1:300 dilution), Fibrillarin (H-140) (SC-25397, Santa Cruz Biotechnology Inc., 1:50 dilution), Incubation:Article Title: MYCN-induced nucleolar stress drives an early senescence-like transcriptional program in hTERT-immortalized RPE cells. Article Snippet: Subsequently, cells were permeabilized with 0.3% Triton X-100 (8 min) and washed (2 × 5 min), after which a blocking step was added with 50% FBS for 30 min and incubated with primary antibody diluted in 50% FBS for 60 min. After minimally 3 washing steps with PBS, plates were incubated with secondary antibody diluted in 50% FBS for 60 min, washed again, and counterstained with DAPI (5 μg/ml) for 20 min. Primary antibodies were directed against p21 (F-5) (SC-6246, Santa Cruz Biotechnology Inc., 1:100 dilution), p53 (7F5) (#2527 T, Cell Signalling Technology, 1:1600 dilution), Lamin B1 (ab16048, Abcam, 1:1000 dilution), Ki-67 (M7240) (M724029-2, Agilent, 1:400 dilution), γH2AX (phospho-S139) (ab2893, Abcam, 1:1000 dilution), RPA32/RPA2 (9H8) (ab2175, Abcam, 1:300 dilution), Fibrillarin (H-140) (SC-25397, Santa Cruz Biotechnology Inc., 1:50 dilution), DDX6 (200–192, Novus, 1:300 dilution) and c a Fibrillarin 24 h 48 h 72 h * * MYCN-ONMYCN-OFF FIBRILLARIN DAPI MERGED M Y C N -O F F M Y C N -O N b 5 10 15 0 10 20 30 N uc le ol ar A re a S in gl e N uc le ol us F ra ct io n MYCN-OFF MYCN-ON 24 h 48 h 72 h *n. s. n. s. n. s. d 10 20 30 40 24 h C el ls w ith 1 n uc le ol us ( % ) 10 20 30 40 48 h 10 20 30 40 72 h MYCN-OFF MYCN-ON G1 S G2 G1 S G2 G1 S G2 *n. s. * * ** ** n. s. *n. s. Fibrillarin/DAPI Figure 6. .. Subsequently, cells were permeabilized with 0.3% Triton X-100 (8 min) and washed (2 × 5 min), after which a blocking step was added with 50% FBS for 30 min and incubated with primary antibody diluted in 50% FBS for 60 min. After minimally 3 washing steps with PBS, plates were incubated with secondary antibody diluted in 50% FBS for 60 min, washed again, and counterstained with DAPI (5 μg/ml) for 20 min. Primary antibodies were directed against p21 (F-5) (SC-6246, Santa Cruz Biotechnology Inc., 1:100 dilution), p53 (7F5) (#2527 T, Cell Signalling Technology, 1:1600 dilution), Lamin B1 (ab16048, Abcam, 1:1000 dilution), Ki-67 (M7240) (M724029-2, Agilent, 1:400 dilution), γH2AX (phospho-S139) (ab2893, Abcam, 1:1000 dilution), RPA32/RPA2 (9H8) (ab2175, Abcam, 1:300 dilution), Fibrillarin (H-140) (SC-25397, Santa Cruz Biotechnology Inc., 1:50 dilution), Article Title: Combinatorial microRNA activity is essential for the transition of pluripotent cells from proliferation into dormancy. Article Snippet: After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum; Jackson Immunoresearch/Dianova 017-000-121) for 1 h at RT. .. Cells were then stained with the primary antibodies PML 1:200 (Novus NB100-59787), SRSF2 1:2000 (Sigma-Aldrich S4045), and Staining:Article Title: Combinatorial microRNA activity is essential for the transition of pluripotent cells from proliferation into dormancy. Article Snippet: After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum; Jackson Immunoresearch/Dianova 017-000-121) for 1 h at RT. .. Cells were then stained with the primary antibodies PML 1:200 (Novus NB100-59787), SRSF2 1:2000 (Sigma-Aldrich S4045), and |
