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ec2 3 dcas9 mxi1 sgrna dcas9 mxi1 expression vector  (Addgene inc)


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    Structured Review

    Addgene inc ec2 3 dcas9 mxi1 sgrna dcas9 mxi1 expression vector
    Ec2 3 Dcas9 Mxi1 Sgrna Dcas9 Mxi1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcas9+expression+vector/pcDNA3%2E1+Orai3+(Plasmid+%2316370)/pmc12645569-47-1-5
    Average 93 stars, based on 5 article reviews
    ec2 3 dcas9 mxi1 sgrna dcas9 mxi1 expression vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Artificial escape from XCI by DNA methylation editing of the CDKL5 gene.
    Article Snippet: For gene expression modulation experiments, cells per well were grown to 80% confluency and transfected within 24 h of plating using Lipofectamine 3000 (Life Technologies) following the manufacturer’s instructions with 3 ul of Lipofectamine 3000 reagent diluted in 500 ul Opti-MEM reduced serum media (Thermo Fisher Scientific). .. Transfections were performed in 12-well plates using either a mock-treatment (diluted transfection reagent) or 700 ng dCas9 expression vector (Fuw-dCas9-Tet1CD-P2A-BFP, Addgene plasmid #108245; Fuw-dCas9-Tet1CD IM, Addgene plasmid # 84479; pLV hUbC-dCas9-T2A-GFP, Addgene plasmid # 53191; pLV hUbC-dCas9 VP64-T2A-GFP, Addgene plasmid # 53192) and 300 ng of equimolar pooled sgRNA expression vectors. ..

    Article Title: Artificial escape from XCI by DNA methylation editing of the CDKL5 gene
    Article Snippet: For gene expression modulation experiments, cells per well were grown to 80% confluency and transfected within 24 h of plating using Lipofectamine 3000 (Life Technologies) following the manufacturer's instructions with 3 ul of Lipofectamine 3000 reagent diluted in 500 ul Opti-MEM reduced serum media (Thermo Fisher Scientific). .. Transfections were performed in 12-well plates using either a mock-treatment (diluted transfection reagent) or 700 ng dCas9 expression vector (Fuw-dCas9-Tet1CD-P2A-BFP, Addgene plasmid #108245; Fuw-dCas9-Tet1CD_IM, Addgene plasmid # 84479; pLV hUbC-dCas9-T2A-GFP, Addgene plasmid # 53191; pLV hUbC-dCas9 VP64-T2A-GFP, Addgene plasmid # 53192) and 300 ng of equimolar pooled sgRNA expression vectors. ..

    Expressing:

    Article Title: Artificial escape from XCI by DNA methylation editing of the CDKL5 gene.
    Article Snippet: For gene expression modulation experiments, cells per well were grown to 80% confluency and transfected within 24 h of plating using Lipofectamine 3000 (Life Technologies) following the manufacturer’s instructions with 3 ul of Lipofectamine 3000 reagent diluted in 500 ul Opti-MEM reduced serum media (Thermo Fisher Scientific). .. Transfections were performed in 12-well plates using either a mock-treatment (diluted transfection reagent) or 700 ng dCas9 expression vector (Fuw-dCas9-Tet1CD-P2A-BFP, Addgene plasmid #108245; Fuw-dCas9-Tet1CD IM, Addgene plasmid # 84479; pLV hUbC-dCas9-T2A-GFP, Addgene plasmid # 53191; pLV hUbC-dCas9 VP64-T2A-GFP, Addgene plasmid # 53192) and 300 ng of equimolar pooled sgRNA expression vectors. ..

    Article Title: Transcriptional regulation of Satb1 in mouse trophoblast stem cells
    Article Snippet: .. For CRISPR-interference, Rcho1 cells were co-transfected with the gRNA and dCas9 expression vector (pLV hUbc-dCas9-T2A-GFP; Addgene, Plasmid #53191) ( ). ..

    Article Title: A long-range chromatin interaction regulates SATB homeobox 1 gene expression in trophoblast stem cells
    Article Snippet: .. For CRISPR-interference, Rcho1 cells were co-transfected with the gRNA and dCas9 expression vector (pLV hUbc-dCas9-T2A-GFP; Addgene, Plasmid #53191) [ ]. ..

    Article Title: Artificial escape from XCI by DNA methylation editing of the CDKL5 gene
    Article Snippet: For gene expression modulation experiments, cells per well were grown to 80% confluency and transfected within 24 h of plating using Lipofectamine 3000 (Life Technologies) following the manufacturer's instructions with 3 ul of Lipofectamine 3000 reagent diluted in 500 ul Opti-MEM reduced serum media (Thermo Fisher Scientific). .. Transfections were performed in 12-well plates using either a mock-treatment (diluted transfection reagent) or 700 ng dCas9 expression vector (Fuw-dCas9-Tet1CD-P2A-BFP, Addgene plasmid #108245; Fuw-dCas9-Tet1CD_IM, Addgene plasmid # 84479; pLV hUbC-dCas9-T2A-GFP, Addgene plasmid # 53191; pLV hUbC-dCas9 VP64-T2A-GFP, Addgene plasmid # 53192) and 300 ng of equimolar pooled sgRNA expression vectors. ..

    Plasmid Preparation:

    Article Title: Artificial escape from XCI by DNA methylation editing of the CDKL5 gene.
    Article Snippet: For gene expression modulation experiments, cells per well were grown to 80% confluency and transfected within 24 h of plating using Lipofectamine 3000 (Life Technologies) following the manufacturer’s instructions with 3 ul of Lipofectamine 3000 reagent diluted in 500 ul Opti-MEM reduced serum media (Thermo Fisher Scientific). .. Transfections were performed in 12-well plates using either a mock-treatment (diluted transfection reagent) or 700 ng dCas9 expression vector (Fuw-dCas9-Tet1CD-P2A-BFP, Addgene plasmid #108245; Fuw-dCas9-Tet1CD IM, Addgene plasmid # 84479; pLV hUbC-dCas9-T2A-GFP, Addgene plasmid # 53191; pLV hUbC-dCas9 VP64-T2A-GFP, Addgene plasmid # 53192) and 300 ng of equimolar pooled sgRNA expression vectors. ..

    Article Title: Transcriptional regulation of Satb1 in mouse trophoblast stem cells
    Article Snippet: .. For CRISPR-interference, Rcho1 cells were co-transfected with the gRNA and dCas9 expression vector (pLV hUbc-dCas9-T2A-GFP; Addgene, Plasmid #53191) ( ). ..

    Article Title: A long-range chromatin interaction regulates SATB homeobox 1 gene expression in trophoblast stem cells
    Article Snippet: .. For CRISPR-interference, Rcho1 cells were co-transfected with the gRNA and dCas9 expression vector (pLV hUbc-dCas9-T2A-GFP; Addgene, Plasmid #53191) [ ]. ..

    Article Title: Artificial escape from XCI by DNA methylation editing of the CDKL5 gene
    Article Snippet: For gene expression modulation experiments, cells per well were grown to 80% confluency and transfected within 24 h of plating using Lipofectamine 3000 (Life Technologies) following the manufacturer's instructions with 3 ul of Lipofectamine 3000 reagent diluted in 500 ul Opti-MEM reduced serum media (Thermo Fisher Scientific). .. Transfections were performed in 12-well plates using either a mock-treatment (diluted transfection reagent) or 700 ng dCas9 expression vector (Fuw-dCas9-Tet1CD-P2A-BFP, Addgene plasmid #108245; Fuw-dCas9-Tet1CD_IM, Addgene plasmid # 84479; pLV hUbC-dCas9-T2A-GFP, Addgene plasmid # 53191; pLV hUbC-dCas9 VP64-T2A-GFP, Addgene plasmid # 53192) and 300 ng of equimolar pooled sgRNA expression vectors. ..

    CRISPR:

    Article Title: Transcriptional regulation of Satb1 in mouse trophoblast stem cells
    Article Snippet: .. For CRISPR-interference, Rcho1 cells were co-transfected with the gRNA and dCas9 expression vector (pLV hUbc-dCas9-T2A-GFP; Addgene, Plasmid #53191) ( ). ..

    Article Title: A long-range chromatin interaction regulates SATB homeobox 1 gene expression in trophoblast stem cells
    Article Snippet: .. For CRISPR-interference, Rcho1 cells were co-transfected with the gRNA and dCas9 expression vector (pLV hUbc-dCas9-T2A-GFP; Addgene, Plasmid #53191) [ ]. ..



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    Figure 1. Effects of different sequences of sgRNAs (sgRNA1-3), including the vector and positive control sgRNA, on gene expression according to RT‒qPCR at 0 and 24 h. (a-e) Fold changes in the dCas9 gene. (f-g) Fold changes in the number of cells positive for the mmpL3 gene. (h-k) Fold changes in the inhA gene. (l) Comparison of the fold changes in inhA gene expression after 24 h of aTc activation. Statistical significance was determined using nonparametric tests (Mann‒Whitney U test) for comparisons between inactivated and activated samples using 100 ng/ml of aTc at each time point for different strains (a-k). The Kruskal‒Wallis test was performed to analyze the significant differences in inhA gene expression from different sgRNAs after induction by aTc for 24 h (l). Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Journal: Scientific reports

    Article Title: The CRISPR-dCas9 interference system suppresses inhA gene expression in Mycobacterium smegmatis.

    doi: 10.1038/s41598-024-77442-2

    Figure Lengend Snippet: Figure 1. Effects of different sequences of sgRNAs (sgRNA1-3), including the vector and positive control sgRNA, on gene expression according to RT‒qPCR at 0 and 24 h. (a-e) Fold changes in the dCas9 gene. (f-g) Fold changes in the number of cells positive for the mmpL3 gene. (h-k) Fold changes in the inhA gene. (l) Comparison of the fold changes in inhA gene expression after 24 h of aTc activation. Statistical significance was determined using nonparametric tests (Mann‒Whitney U test) for comparisons between inactivated and activated samples using 100 ng/ml of aTc at each time point for different strains (a-k). The Kruskal‒Wallis test was performed to analyze the significant differences in inhA gene expression from different sgRNAs after induction by aTc for 24 h (l). Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Article Snippet: The PLJR962 CRISPRi vector expressing Streptococcus thermophilus dCas9 (SthdCas9) with a specific site for inserted sgRNAs (Addgene plasmid #115162) was kindly provided by Dr. Sarah Fortune (Department of Immunology and Infectious Diseases, Harvard T.H.

    Techniques: Plasmid Preparation, Positive Control, Gene Expression, Comparison, Activation Assay, Standard Deviation

    Figure 3. Effects of different aTc concentrations for CRISPRi activation on gene expression, as determined by RT‒qPCR at 0 and 24 h. (a-c) Fold changes in the dCas9 gene. (d-e) Fold changes in the number of positive sgRNAs and mmpL3 gene expression. (f-g) Fold changes in the inhA gene. Statistical significance was determined using nonparametric ANOVA (Kruskal‒Wallis test) to compare Msm inactivated and activated by 50, 100 or 200 ng/ml of aTc at each time point with the vector control, positive sgRNA, or sgRNA2. Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Journal: Scientific reports

    Article Title: The CRISPR-dCas9 interference system suppresses inhA gene expression in Mycobacterium smegmatis.

    doi: 10.1038/s41598-024-77442-2

    Figure Lengend Snippet: Figure 3. Effects of different aTc concentrations for CRISPRi activation on gene expression, as determined by RT‒qPCR at 0 and 24 h. (a-c) Fold changes in the dCas9 gene. (d-e) Fold changes in the number of positive sgRNAs and mmpL3 gene expression. (f-g) Fold changes in the inhA gene. Statistical significance was determined using nonparametric ANOVA (Kruskal‒Wallis test) to compare Msm inactivated and activated by 50, 100 or 200 ng/ml of aTc at each time point with the vector control, positive sgRNA, or sgRNA2. Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Article Snippet: The PLJR962 CRISPRi vector expressing Streptococcus thermophilus dCas9 (SthdCas9) with a specific site for inserted sgRNAs (Addgene plasmid #115162) was kindly provided by Dr. Sarah Fortune (Department of Immunology and Infectious Diseases, Harvard T.H.

    Techniques: Activation Assay, Gene Expression, Plasmid Preparation, Control, Standard Deviation