Journal: Antimicrobial Agents and Chemotherapy

Article Title: Polygenic, cell-envelope adaptations drive high-frequency daptomycin resistance in Staphylococcus capitis NRCS-A from neonatal sepsis and NEC

doi: 10.1128/aac.01414-25

Figure Lengend Snippet: Daptomycin and vancomycin susceptibility of S. capitis NRCS-A isolates (ScSK) from our local collection as determined by MIC test strip. MICs of ( A ) daptomycin and ( B ) vancomycin are shown. Black lines indicate cut-off concentrations for resistance: 1 μg/mL for daptomycin, 2 μg/mL for vancomycin reduced susceptibility (cut-off for S. aureus ), and 4 μg/mL for vancomycin resistance (EUCAST definition, v15.0 of 2025). The S. aureus SA113 WT and SA113 ∆ mprF (gray), which is more susceptible to daptomycin and vancomycin, were used as controls. NRCS-A isolates (light blue) were compared with non-invasive S. capitis strain DSM6717 (dark blue) and the S. capitis bloodstream isolate from a toddler’s catheter sepsis (ScSK11, turquoise). The mean ± SEM of at least three independent experiments is shown.

Article Snippet: Therefore, MIC test strips for daptomycin and vancomycin (Liofilchem) were applied on MHB agar plates and analyzed after 24 h incubation at 37°C according to the manufacturer’s protocol.

Techniques: Stripping Membranes

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Polygenic, cell-envelope adaptations drive high-frequency daptomycin resistance in Staphylococcus capitis NRCS-A from neonatal sepsis and NEC

doi: 10.1128/aac.01414-25

Figure Lengend Snippet: ( A ) Binding of BODIPY-labeled daptomycin (BODIPY-Dap) in the previously determined subinhibitory concentration of 7.81 µg/mL to the S. capitis NRCS-A isolate ScSK1 (light blue) and the non-invasive S. capitis DSM6717 (dark blue). Bacterial cells were incubated with BODIPY-Dap and analyzed by flow cytometry (FITC channel) at 0 h, 0.5 h, 1 h, and 2 h. Statistical analysis was performed using two-way ANOVA with Šidák’s multiple-comparisons test to compare BODIPY-Dap binding between strains at each time point ( P < 0.05, * P < 0.01, ** P < 0.001). Data are shown as mean ± SD from at least four independent experiments. ( B ) Fluorescence microscopy of S. capitis strains DSM6717 and ScSK1 after incubation with BODIPY-Dap for 0, 10, 30, and 60 min. Representative images were acquired on a Zeiss LSM 800 confocal microscope using a 100× objective. Scale bar, 2 µm.

Article Snippet: Therefore, MIC test strips for daptomycin and vancomycin (Liofilchem) were applied on MHB agar plates and analyzed after 24 h incubation at 37°C according to the manufacturer’s protocol.

Techniques: Binding Assay, Labeling, Concentration Assay, Incubation, Flow Cytometry, Fluorescence, Microscopy

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Polygenic, cell-envelope adaptations drive high-frequency daptomycin resistance in Staphylococcus capitis NRCS-A from neonatal sepsis and NEC

doi: 10.1128/aac.01414-25

Figure Lengend Snippet: DAP-R evolution of S. capitis NRCS-A (ScSK4) in light blue compared to non-invasive S. capitis (DSM6717) in dark blue, S. aureus (USA300) in gray, and S. epidermidis (SeSK1) in white. Strains were grown without ( A ) or with ( B ) daptomycin pressure or ( C ) vancomycin pressure in MHB for 24 h followed by daptomycin MIC determination after each passage via E-tests. Black lines indicate cut-off concentrations for daptomycin resistance at 1 μg/mL (EUCAST definition, v15.0 of 2025). The black arrows highlight the passaging days on which bacterial strains evolved a DAP-R phenotype.

Article Snippet: Therefore, MIC test strips for daptomycin and vancomycin (Liofilchem) were applied on MHB agar plates and analyzed after 24 h incubation at 37°C according to the manufacturer’s protocol.

Techniques: Passaging

Journal: The Journal of Infectious Diseases

Article Title: [ 18 F]Fluorodeoxyglucose Positron Emission Tomography for Diagnosis and Monitoring of Acute Staphylococcus aureus Vascular Graft Infection in a Rat Model

doi: 10.1093/infdis/jiaf594

Figure Lengend Snippet: Images from PET scan of individual rats from each group. The arrow indicates the location of the implant. ( A ) S. aureus -infected rats at days 10, 20, and 31. ( B ) Uninfected rats at days 10, 20, and 31. ( C ) S. epidermidis -infected rat at days 7, 17, and 31. Images from S. epidermidis -infected rat are from the same rat at different time points. ( D ) S. aureus -infected rat treated with daptomycin and rifampicin on day 31.

Article Snippet: The antibiotics were daptomycin (“Cubicin”, Merck Sharp & Dohme, Copenhagen, Denmark), rifampicin (“Rifadin”, Sanofi S.r.I., Milan, Italy), and a single dose of preoperatively administered cefuroxime (“Cefuroxim”, Fresenius Kabi, Copenhagen, Denmark).

Techniques: Infection

Journal: The Journal of Infectious Diseases

Article Title: [ 18 F]Fluorodeoxyglucose Positron Emission Tomography for Diagnosis and Monitoring of Acute Staphylococcus aureus Vascular Graft Infection in a Rat Model

doi: 10.1093/infdis/jiaf594

Figure Lengend Snippet: Colony-forming units (CFU) for each group. Data points represent mean CFU of 3 technical replicates from 1 implant. Bars represent median. Kruskal-Wallis test with post hoc Dunn's test. Uninfected, day 10 (n = 7). S. epidermidis , day 7 (n = 6). S. aureus , day 10 (n = 7). Uninfected, day 20 (n = 7). S. epidermidis , day 17 (n = 7). S. aureus , day 20 (n = 8). Uninfected, day 31 (n = 8). S. epidermidis , day 31 (n = 4). S. aureus , day 31 (n = 6). S. aureus DAP + RIF, day 31 (n = 8). DAP/RIF = daptomycin + rifampicin; SA = S. aureus ; SE = S. epidermidis ; UI = uninfected.

Article Snippet: The antibiotics were daptomycin (“Cubicin”, Merck Sharp & Dohme, Copenhagen, Denmark), rifampicin (“Rifadin”, Sanofi S.r.I., Milan, Italy), and a single dose of preoperatively administered cefuroxime (“Cefuroxim”, Fresenius Kabi, Copenhagen, Denmark).

Techniques:

Journal: The Journal of Infectious Diseases

Article Title: [ 18 F]Fluorodeoxyglucose Positron Emission Tomography for Diagnosis and Monitoring of Acute Staphylococcus aureus Vascular Graft Infection in a Rat Model

doi: 10.1093/infdis/jiaf594

Figure Lengend Snippet: Interleukin-10 (IL-10) concentrations in plasma. Each point shows mean value of 1–2 technical replicates from 1 rat. Bars represent median. The lower limit of detection was 7.278 pg/ml. Plasma samples below the measurable concentration were set at a default 7.278 pg/ml to avoid skewing of data. Kruskal-Wallis with post hoc Dunn's test of day 31 groups. DAP/RIF = daptomycin + rifampicin; SA = S. aureus ; SE = S. epidermidis ; UI = uninfected.

Article Snippet: The antibiotics were daptomycin (“Cubicin”, Merck Sharp & Dohme, Copenhagen, Denmark), rifampicin (“Rifadin”, Sanofi S.r.I., Milan, Italy), and a single dose of preoperatively administered cefuroxime (“Cefuroxim”, Fresenius Kabi, Copenhagen, Denmark).

Techniques: Clinical Proteomics, Concentration Assay