Journal: Colloid and Interface Science Communications

Article Title: Colistin does not self-assemble at physiologically relevant conditions

doi: 10.1016/j.colcom.2025.100824

Figure Lengend Snippet: Fig. 2. A) FoXS [34] fit based only on the reported PDB structure of colistin to the experimental SAXS curve of colistin in PBS at 3 mg/mL. B) SAXS curves of colistin in PBS measured at 37 ◦C over a range of different concentrations. All curves are on absolute scale, and simultaneous model fits of unimeric Gaussian chains are indicated by solid lines. C) Zimm plot of the same experimental SAXS curves shown in panel B, used to determine the radius of gyration and molecular weight of the scatterers. D) SAXS curves of the daptomycin as a positive control showing self-assembled lipopeptide micelles in solution at 5 mg/mL and containing 4.6 mM CaCl2. The daptomycin micelles (red points) are described using an ellipsoidal core-shell model, while daptomycin below the CMC (orange points) and colistin in PBS (blue points) are shown for reference, and described as Gaussian chains using the Debye model. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Daptomycin, which was used as a positive control to show self-assembling lipopeptides, was purchased at Selleckchem.

Techniques: Molecular Weight, Positive Control

Journal: Journal of Drug Delivery Science and Technology

Article Title: Synergistic antimicrobial effect of daptomycin and ethyl lauroyl arginate containing self-emulsifying drug delivery system against bacterial infections

doi: 10.1016/j.jddst.2024.106324

Figure Lengend Snippet: Fig. 1. (A) Log P octanol/water for daptomycin and HIP in different molar ratios daptomycin to ELA, (B) efficiency of hydrophobic complexes formed with peptide and ELA via the organic solvent-free method. Sodium hydroxide was used to adjust the pH of the aqueous phase to 7.0. Data are presented as means ± SD, n = 3. Significant differences are indicated as **p < 0.01; ***p < 0.001.

Article Snippet: Daptomycin was obtained from Biosynth AG (Staad, Switzerland).

Techniques: Solvent

Journal: Journal of Drug Delivery Science and Technology

Article Title: Synergistic antimicrobial effect of daptomycin and ethyl lauroyl arginate containing self-emulsifying drug delivery system against bacterial infections

doi: 10.1016/j.jddst.2024.106324

Figure Lengend Snippet: Fig. 5. Inhibition zones of daptomycin, ELA, HIP, and SEDDS against different bacteria and fungi. The disk diffusion method was employed, where 6 mm discs impregnated with different formulations were placed on agar plates inoculated with microorganisms. After 24 h of incubation at 37 ◦C, inhibition zones were recorded in millimeters. Data represent the mean ± SD, n = 3.

Article Snippet: Daptomycin was obtained from Biosynth AG (Staad, Switzerland).

Techniques: Inhibition, Bacteria, Diffusion-based Assay, Incubation

Journal: Journal of Drug Delivery Science and Technology

Article Title: Synergistic antimicrobial effect of daptomycin and ethyl lauroyl arginate containing self-emulsifying drug delivery system against bacterial infections

doi: 10.1016/j.jddst.2024.106324

Figure Lengend Snippet: Fig. 6. Killing curve of (A) S. aureus and (B) S. epidermidis. Control was in water/Tryptic soy broth solution (1:1 v/v) without daptomycin. Data are shown as a means (n = 3) ± SD (*p < 0.05, **p < 0.01, ***p < 0.001 in comparison to free daptomycin).

Article Snippet: Daptomycin was obtained from Biosynth AG (Staad, Switzerland).

Techniques: Control, Comparison

Journal: Computers in Biology and Medicine

Article Title: Drug repurposing against SARS-CoV-2 receptor binding domain using ensemble-based virtual screening and molecular dynamics simulations

doi: 10.1016/j.compbiomed.2021.104634

Figure Lengend Snippet: Comparison of present study with published studies that report repurposed drugs/compounds against SARS-CoV-2 Spike RBD. Studies have been grouped into four categories, i.e., category 1 includes simple structure or pharmacophore-based studies, category 2 includes virtual screening against single RBD conformation with MD validation of binding poses of selected hits, category 3 includes virtual screening against multiple conformations (ensemble) of RBD but no MD validation of binding poses and category 4 includes ensemble-based virtual screening with MD validation of binding poses of selected hits.

Article Snippet: MD Simulation of hACE2-RBD complex (20 ns) + Structure-based virtual screening + MD Simulations of RBD-Drug Complexes (60 × 1 ns = 60 ns) , 2 , Crystal Structure (PDB ID: 6M0J ) , SelleckChem and Targetmol , Polymixin B, Colistin, Daptomycin, Thymopentin and Icatibant , Maffucci and Contini [ ] .

Techniques: Binding Assay, Generated