Journal: Biochimica et biophysica acta

Article Title: Rapid bactericidal action of alpha-mangostin against MRSA as an outcome of membrane targeting.

doi: 10.1016/j.bbamem.2012.09.004

Figure Lengend Snippet: Fig. 5. α-Mangostin as a cytoplasmic membrane disruption agent. (A) Effects of α-mangostin with different concentrations on the fluorescence intensity of EtBr incu- bated with clinical isolate S. aureus DM4001 at 0.25×, 0.5×, 1×, 2×, 4× and 8× MICs. (B) Effects of different antibiotics on the EtBr fluorescence intensity: red line, 1 μL DMF; black line, α-mangostin, 8× MIC; blue line, daptomycin, 12.5 μg/mL; light blue line, vancomycin, 8× MIC; and purple line, 15 μL triton X-100. MIC values (μg/mL): α-mangostin=1.56; vancomycin=1.56. cps: count per second.

Article Snippet: Daptomycin was purchased from Tocris Bioscience, United Kingdom.

Techniques: Membrane, Disruption

Journal: Frontiers in Microbiology

Article Title: Genome-Wide Identification of Antimicrobial Intrinsic Resistance Determinants in Staphylococcus aureus

doi: 10.3389/fmicb.2016.02018

Figure Lengend Snippet: Gene inactivations affecting susceptibility to antimicrobial agents to one or more of the eight antimicrobial agents tested. The effect on susceptibility is displayed using a gray scale indicating fold reductions in sensitivity: , and . The genes are divided into the categories: (1) Membrane transporters, (2) Metabolism, (3) Replication and DNA repair, (4) Pro-phage encoded genes, (5) Protein synthesis, (6) Transcriptional regulators, chaperones, cell wall and membrane stress mechanisms and (7) Unassigned and hypothetical genes. CIP (Ciprofloxacin), OXA (Oxacillin), LZD (Linezolid), FOF (Fosfomycin), DAP (Daptomycin), MUP (Mupirocin), VAN (Vancomycin) and GEN (Gentamicin).

Article Snippet: Antimicrobial agents used in the study were erythromycin (Sigma), ciprofloxacin (Sigma), oxacillin (Sigma), linezolid (Sigma), fosfomycin (Sigma), daptomycin (Santa Cruz Biotechnology), mupirocin (Sigma), vancomycin (Sigma) and gentamicin (Sigma).

Techniques: Membrane

Journal: Frontiers in Microbiology

Article Title: Genome-Wide Identification of Antimicrobial Intrinsic Resistance Determinants in Staphylococcus aureus

doi: 10.3389/fmicb.2016.02018

Figure Lengend Snippet: Antimicrobial agents used in the screening for intrinsic resistance determinants, as well as the primary target and process affected by the respective agents.

Article Snippet: Antimicrobial agents used in the study were erythromycin (Sigma), ciprofloxacin (Sigma), oxacillin (Sigma), linezolid (Sigma), fosfomycin (Sigma), daptomycin (Santa Cruz Biotechnology), mupirocin (Sigma), vancomycin (Sigma) and gentamicin (Sigma).

Techniques: Membrane, Permeability

Journal: Journal of Drug Delivery Science and Technology

Article Title: Synergistic antimicrobial effect of daptomycin and ethyl lauroyl arginate containing self-emulsifying drug delivery system against bacterial infections

doi: 10.1016/j.jddst.2024.106324

Figure Lengend Snippet: Fig. 1. (A) Log P octanol/water for daptomycin and HIP in different molar ratios daptomycin to ELA, (B) efficiency of hydrophobic complexes formed with peptide and ELA via the organic solvent-free method. Sodium hydroxide was used to adjust the pH of the aqueous phase to 7.0. Data are presented as means ± SD, n = 3. Significant differences are indicated as **p < 0.01; ***p < 0.001.

Article Snippet: Daptomycin was obtained from Biosynth AG (Staad, Switzerland).

Techniques: Solvent

Journal: Journal of Drug Delivery Science and Technology

Article Title: Synergistic antimicrobial effect of daptomycin and ethyl lauroyl arginate containing self-emulsifying drug delivery system against bacterial infections

doi: 10.1016/j.jddst.2024.106324

Figure Lengend Snippet: Fig. 5. Inhibition zones of daptomycin, ELA, HIP, and SEDDS against different bacteria and fungi. The disk diffusion method was employed, where 6 mm discs impregnated with different formulations were placed on agar plates inoculated with microorganisms. After 24 h of incubation at 37 ◦C, inhibition zones were recorded in millimeters. Data represent the mean ± SD, n = 3.

Article Snippet: Daptomycin was obtained from Biosynth AG (Staad, Switzerland).

Techniques: Inhibition, Bacteria, Diffusion-based Assay, Incubation

Journal: Journal of Drug Delivery Science and Technology

Article Title: Synergistic antimicrobial effect of daptomycin and ethyl lauroyl arginate containing self-emulsifying drug delivery system against bacterial infections

doi: 10.1016/j.jddst.2024.106324

Figure Lengend Snippet: Fig. 6. Killing curve of (A) S. aureus and (B) S. epidermidis. Control was in water/Tryptic soy broth solution (1:1 v/v) without daptomycin. Data are shown as a means (n = 3) ± SD (*p < 0.05, **p < 0.01, ***p < 0.001 in comparison to free daptomycin).

Article Snippet: Daptomycin was obtained from Biosynth AG (Staad, Switzerland).

Techniques: Control, Comparison

Journal: Computers in Biology and Medicine

Article Title: Drug repurposing against SARS-CoV-2 receptor binding domain using ensemble-based virtual screening and molecular dynamics simulations

doi: 10.1016/j.compbiomed.2021.104634

Figure Lengend Snippet: Comparison of present study with published studies that report repurposed drugs/compounds against SARS-CoV-2 Spike RBD. Studies have been grouped into four categories, i.e., category 1 includes simple structure or pharmacophore-based studies, category 2 includes virtual screening against single RBD conformation with MD validation of binding poses of selected hits, category 3 includes virtual screening against multiple conformations (ensemble) of RBD but no MD validation of binding poses and category 4 includes ensemble-based virtual screening with MD validation of binding poses of selected hits.

Article Snippet: MD Simulation of hACE2-RBD complex (20 ns) + Structure-based virtual screening + MD Simulations of RBD-Drug Complexes (60 × 1 ns = 60 ns) , 2 , Crystal Structure (PDB ID: 6M0J ) , SelleckChem and Targetmol , Polymixin B, Colistin, Daptomycin, Thymopentin and Icatibant , Maffucci and Contini [ ] .

Techniques: Binding Assay, Generated