Journal: Antimicrobial Agents and Chemotherapy

Article Title: Polygenic, cell-envelope adaptations drive high-frequency daptomycin resistance in Staphylococcus capitis NRCS-A from neonatal sepsis and NEC

doi: 10.1128/aac.01414-25

Figure Lengend Snippet: ( A ) Binding of BODIPY-labeled daptomycin (BODIPY-Dap) in the previously determined subinhibitory concentration of 7.81 µg/mL to the S. capitis NRCS-A isolate ScSK1 (light blue) and the non-invasive S. capitis DSM6717 (dark blue). Bacterial cells were incubated with BODIPY-Dap and analyzed by flow cytometry (FITC channel) at 0 h, 0.5 h, 1 h, and 2 h. Statistical analysis was performed using two-way ANOVA with Šidák’s multiple-comparisons test to compare BODIPY-Dap binding between strains at each time point ( P < 0.05, * P < 0.01, ** P < 0.001). Data are shown as mean ± SD from at least four independent experiments. ( B ) Fluorescence microscopy of S. capitis strains DSM6717 and ScSK1 after incubation with BODIPY-Dap for 0, 10, 30, and 60 min. Representative images were acquired on a Zeiss LSM 800 confocal microscope using a 100× objective. Scale bar, 2 µm.

Article Snippet: BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacen) (Invitrogen/Thermo-Fisher, D3922) labeling of daptomycin was performed as described previously ( , ).

Techniques: Binding Assay, Labeling, Concentration Assay, Incubation, Flow Cytometry, Fluorescence, Microscopy

Journal: bioRxiv

Article Title: Modular biomaterials vaccine technology protects against multiple pathogens and septic shock

doi: 10.1101/2020.02.25.964601

Figure Lengend Snippet: ciVAX was produced by mixing lyophilized MPS biomaterial scaffold containing murine GMCSF and CpG with vancomycin & daptomycin-killed MRSA (ATCC 12598) captured on FcMBL paramagnetic MyOne Beads. (A) 8-10 week old female BALB/c mice were vaccinated & boosted (day 14) by subcutaneous injection with ciVAX (orange), or sham scaffold without the FcMBL/RS218 (blue), or unvaccinated control (gray) (n=10 per group). MRSA-specific antibodies in mouse serum were measured using vancomycin & daptomycin-killed MRSA adsorbed directly on well-plates, blocked to reduce interaction between Staphylococcal protein A and IgG. (B) Mice were challenged 35 days after vaccination with 5e7 CFU MRSA on the left flank and monitored for abscess formation for a further 72 hours. Data are mean and standard deviation; statistically significant differences between the independent groups were identified by non-parametric Mann-Whitney U test.

Article Snippet: Next, 1mg/mL of cefepime (NDC 25021-121-20) was added to E. coli , 1 mg/mL vancomycin (NDC 0409-4332-49) and 0.5 mg/mL daptomycin (Toronto Research Chemicals, Canada) was added to S. aureus .

Techniques: Produced, Injection, Control, Standard Deviation, MANN-WHITNEY

Journal: Science Advances

Article Title: Globally deimmunized lysostaphin evades human immune surveillance and enables highly efficacious repeat dosing

doi: 10.1126/sciadv.abb9011

Figure Lengend Snippet: Daptomycin dosed at 4 mg/kg once daily × 4 days, F12 dosed at 40 mg/kg once daily × 1 day, or a combined treatment using the same dosing regimens. Viable bacterial counts recovered from ( A ) cardiac vegetations, ( B ) kidney, and ( C ) spleen at the study conclusion are shown. Lines and bars are mean and SD. The combination therapy eradicated bacteria in all target tissues such that the limit of detection was assigned as an upper bound. The combination therapy is significantly different from all other groups (two-way ANOVA). See table S7 for summary statistics. Mortality was 100% for control animals, 14% for daptomycin, 20% for F12, and 0% for the combination therapy.

Article Snippet: Daptomycin was purchased from Selleck Chemicals.

Techniques: Bacteria, Control

Journal: PLoS ONE

Article Title: Use of a Bacteriophage Lysin to Identify a Novel Target for Antimicrobial Development

doi: 10.1371/journal.pone.0060754

Figure Lengend Snippet: Spontaneous antimicrobial resistance.

Article Snippet: For analysis of spontaneous resistance to epimerox, rifampin (Sigma-Aldrich), and daptomycin (Tocris Bioscience), strains were grown in 100 ml BHI with agitation at 30°C ( B. anthracis Sterne and derivatives thereof) or 37°C ( S. aureus RN4220).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Genome-Wide Identification of Antimicrobial Intrinsic Resistance Determinants in Staphylococcus aureus

doi: 10.3389/fmicb.2016.02018

Figure Lengend Snippet: Gene inactivations affecting susceptibility to antimicrobial agents to one or more of the eight antimicrobial agents tested. The effect on susceptibility is displayed using a gray scale indicating fold reductions in sensitivity: , and . The genes are divided into the categories: (1) Membrane transporters, (2) Metabolism, (3) Replication and DNA repair, (4) Pro-phage encoded genes, (5) Protein synthesis, (6) Transcriptional regulators, chaperones, cell wall and membrane stress mechanisms and (7) Unassigned and hypothetical genes. CIP (Ciprofloxacin), OXA (Oxacillin), LZD (Linezolid), FOF (Fosfomycin), DAP (Daptomycin), MUP (Mupirocin), VAN (Vancomycin) and GEN (Gentamicin).

Article Snippet: Antimicrobial agents used in the study were erythromycin (Sigma), ciprofloxacin (Sigma), oxacillin (Sigma), linezolid (Sigma), fosfomycin (Sigma), daptomycin (Santa Cruz Biotechnology), mupirocin (Sigma), vancomycin (Sigma) and gentamicin (Sigma).

Techniques: Membrane

Journal: Frontiers in Microbiology

Article Title: Genome-Wide Identification of Antimicrobial Intrinsic Resistance Determinants in Staphylococcus aureus

doi: 10.3389/fmicb.2016.02018

Figure Lengend Snippet: Antimicrobial agents used in the screening for intrinsic resistance determinants, as well as the primary target and process affected by the respective agents.

Article Snippet: Antimicrobial agents used in the study were erythromycin (Sigma), ciprofloxacin (Sigma), oxacillin (Sigma), linezolid (Sigma), fosfomycin (Sigma), daptomycin (Santa Cruz Biotechnology), mupirocin (Sigma), vancomycin (Sigma) and gentamicin (Sigma).

Techniques: Membrane, Permeability

Journal: Infection and Drug Resistance

Article Title: New Attempts to Inhibit Methicillin-Resistant Staphylococcus aureus Biofilm? A Combination of Daptomycin and Azithromycin

doi: 10.2147/IDR.S433439

Figure Lengend Snippet: The MICs of daptomycin and azithromycin against standard strains of MRSA . ( a ) When the intervention concentration of daptomycin was 8μg/mL, there was no colony growth on the solid medium. When the intervention concentration was 4μg/mL, there was colony growth on the solid medium; ( b ) When the intervention concentration of daptomycin was 1μg/mL and 2μg/mL, there was colony growth on the solid medium; ( c ) When the azithromycin intervention concentration was 512μg/mL and 1042μg/mL, there was no colony growth on the solid medium; ( d ) When azithromycin intervention concentration was 256μg/mL, the colonies were more dispersed than 128μg/mL.

Article Snippet: Reagents and the preparation methods: Daptomycin was purchased from TargetMol, and azithromycin was purchased from MACKLIN.

Techniques: Concentration Assay

Journal: Infection and Drug Resistance

Article Title: New Attempts to Inhibit Methicillin-Resistant Staphylococcus aureus Biofilm? A Combination of Daptomycin and Azithromycin

doi: 10.2147/IDR.S433439

Figure Lengend Snippet: Antibacterial test of daptomycin and azithromycin combined. In the combined inhibition assay, FIC index equals to 1/8 plus 256/512, or 0.625, The combined action of the two drugs on MRSA is additive (each point in the figure are the corresponding concentrations of the two antimicrobial agents when they completely inhibit bacterial growth).

Article Snippet: Reagents and the preparation methods: Daptomycin was purchased from TargetMol, and azithromycin was purchased from MACKLIN.

Techniques: Inhibition

Journal: Infection and Drug Resistance

Article Title: New Attempts to Inhibit Methicillin-Resistant Staphylococcus aureus Biofilm? A Combination of Daptomycin and Azithromycin

doi: 10.2147/IDR.S433439

Figure Lengend Snippet: Semi-Quantitative Results of the Intervention of Daptomycin and Azithromycin on the MRSA Biofilm for 24 h ( \documentclass[12pt]{minimal} \usepackage{wasysym} \usepackage[substack]{amsmath} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage[mathscr]{eucal} \usepackage{mathrsfs} \DeclareFontFamily{T1}{linotext}{} \DeclareFontShape{T1}{linotext}{m}{n} {linotext }{} \DeclareSymbolFont{linotext}{T1}{linotext}{m}{n} \DeclareSymbolFontAlphabet{\mathLINOTEXT}{linotext} \begin{document} $\bar x\pm s$\end{document} )

Article Snippet: Reagents and the preparation methods: Daptomycin was purchased from TargetMol, and azithromycin was purchased from MACKLIN.

Techniques:

Journal: Infection and Drug Resistance

Article Title: New Attempts to Inhibit Methicillin-Resistant Staphylococcus aureus Biofilm? A Combination of Daptomycin and Azithromycin

doi: 10.2147/IDR.S433439

Figure Lengend Snippet: The change in the quantity of live/dead MRSA in the biofilm at 24 hours following treatment with daptomycin and azithromycin alone/combined under CLSM. ( a ) Control group; ( b ) When the intervention concentration of daptomycin was 8μg/mL; ( c ) When the intervention concentration of Daptomycin was 16μg/mL; ( d ) When the azithromycin intervention concentration was 512μg/mL; ( e ) When the concentration was 8μg/mL Daptomycin combined with 512μg/mL azithromycin; ( f ) When the concentration was 16μg/mL daptomycin combined with 512μg/mL azithromycin.

Article Snippet: Reagents and the preparation methods: Daptomycin was purchased from TargetMol, and azithromycin was purchased from MACKLIN.

Techniques: Control, Concentration Assay

Journal: Infection and Drug Resistance

Article Title: New Attempts to Inhibit Methicillin-Resistant Staphylococcus aureus Biofilm? A Combination of Daptomycin and Azithromycin

doi: 10.2147/IDR.S433439

Figure Lengend Snippet: Semi-quantitative outcome of daptomycin and azithromycin at MICs on semi-mature/mature biofilm. The effect of daptomycin and azithromycin on semi-mature biofilm in vitro (black solid line) at MICs demonstrated that the biofilm in the experimental group gradually decreased with time and eventually disappeared, while the semi-mature biofilm in the control group grew to maturity and then became stable over time. The in vitro effect on mature biofilm (red dashed line) showed that the biofilm in the experimental group gradually decreased over time and finally dissipated; while the biofilm in the control group was steady and no tendency for reduction.

Article Snippet: Reagents and the preparation methods: Daptomycin was purchased from TargetMol, and azithromycin was purchased from MACKLIN.

Techniques: In Vitro, Control

Journal: Infection and Drug Resistance

Article Title: New Attempts to Inhibit Methicillin-Resistant Staphylococcus aureus Biofilm? A Combination of Daptomycin and Azithromycin

doi: 10.2147/IDR.S433439

Figure Lengend Snippet: Combination of daptomycin and azithromycin has a negative impact on the mature biofilm of MRSA . After mature biofilms were treated with different concentrations of antibacterial agents for 24 hours, daptomycin had a substantial destroying effect on the biofilm of MRSA ; 43.2% of MRSA biofilm may be destroyed by azithromycin at half its MIC concentration.

Article Snippet: Reagents and the preparation methods: Daptomycin was purchased from TargetMol, and azithromycin was purchased from MACKLIN.

Techniques: Concentration Assay

Journal: Infection and Drug Resistance

Article Title: New Attempts to Inhibit Methicillin-Resistant Staphylococcus aureus Biofilm? A Combination of Daptomycin and Azithromycin

doi: 10.2147/IDR.S433439

Figure Lengend Snippet: Combining daptomycin and azithromycin has a damaging effect on the mature biofilm of MRSA . The biofilm of MRSA could be entirely eliminated by the combination of 1 mg/L daptomycin and 256 mg/L azithromycin as demonstrated by the 24th staining of the two antimicrobial agents on mature biofilms.

Article Snippet: Reagents and the preparation methods: Daptomycin was purchased from TargetMol, and azithromycin was purchased from MACKLIN.

Techniques: Staining