Journal: Antimicrobial Agents and Chemotherapy

Article Title: Polygenic, cell-envelope adaptations drive high-frequency daptomycin resistance in Staphylococcus capitis NRCS-A from neonatal sepsis and NEC

doi: 10.1128/aac.01414-25

Figure Lengend Snippet: ( A ) Binding of BODIPY-labeled daptomycin (BODIPY-Dap) in the previously determined subinhibitory concentration of 7.81 µg/mL to the S. capitis NRCS-A isolate ScSK1 (light blue) and the non-invasive S. capitis DSM6717 (dark blue). Bacterial cells were incubated with BODIPY-Dap and analyzed by flow cytometry (FITC channel) at 0 h, 0.5 h, 1 h, and 2 h. Statistical analysis was performed using two-way ANOVA with Šidák’s multiple-comparisons test to compare BODIPY-Dap binding between strains at each time point ( P < 0.05, * P < 0.01, ** P < 0.001). Data are shown as mean ± SD from at least four independent experiments. ( B ) Fluorescence microscopy of S. capitis strains DSM6717 and ScSK1 after incubation with BODIPY-Dap for 0, 10, 30, and 60 min. Representative images were acquired on a Zeiss LSM 800 confocal microscope using a 100× objective. Scale bar, 2 µm.

Article Snippet: BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacen) (Invitrogen/Thermo-Fisher, D3922) labeling of daptomycin was performed as described previously ( , ).

Techniques: Binding Assay, Labeling, Concentration Assay, Incubation, Flow Cytometry, Fluorescence, Microscopy

Journal: bioRxiv

Article Title: Modular biomaterials vaccine technology protects against multiple pathogens and septic shock

doi: 10.1101/2020.02.25.964601

Figure Lengend Snippet: ciVAX was produced by mixing lyophilized MPS biomaterial scaffold containing murine GMCSF and CpG with vancomycin & daptomycin-killed MRSA (ATCC 12598) captured on FcMBL paramagnetic MyOne Beads. (A) 8-10 week old female BALB/c mice were vaccinated & boosted (day 14) by subcutaneous injection with ciVAX (orange), or sham scaffold without the FcMBL/RS218 (blue), or unvaccinated control (gray) (n=10 per group). MRSA-specific antibodies in mouse serum were measured using vancomycin & daptomycin-killed MRSA adsorbed directly on well-plates, blocked to reduce interaction between Staphylococcal protein A and IgG. (B) Mice were challenged 35 days after vaccination with 5e7 CFU MRSA on the left flank and monitored for abscess formation for a further 72 hours. Data are mean and standard deviation; statistically significant differences between the independent groups were identified by non-parametric Mann-Whitney U test.

Article Snippet: Next, 1mg/mL of cefepime (NDC 25021-121-20) was added to E. coli , 1 mg/mL vancomycin (NDC 0409-4332-49) and 0.5 mg/mL daptomycin (Toronto Research Chemicals, Canada) was added to S. aureus .

Techniques: Produced, Injection, Control, Standard Deviation, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Use of a Bacteriophage Lysin to Identify a Novel Target for Antimicrobial Development

doi: 10.1371/journal.pone.0060754

Figure Lengend Snippet: Spontaneous antimicrobial resistance.

Article Snippet: For analysis of spontaneous resistance to epimerox, rifampin (Sigma-Aldrich), and daptomycin (Tocris Bioscience), strains were grown in 100 ml BHI with agitation at 30°C ( B. anthracis Sterne and derivatives thereof) or 37°C ( S. aureus RN4220).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Genome-Wide Identification of Antimicrobial Intrinsic Resistance Determinants in Staphylococcus aureus

doi: 10.3389/fmicb.2016.02018

Figure Lengend Snippet: Gene inactivations affecting susceptibility to antimicrobial agents to one or more of the eight antimicrobial agents tested. The effect on susceptibility is displayed using a gray scale indicating fold reductions in sensitivity: , and . The genes are divided into the categories: (1) Membrane transporters, (2) Metabolism, (3) Replication and DNA repair, (4) Pro-phage encoded genes, (5) Protein synthesis, (6) Transcriptional regulators, chaperones, cell wall and membrane stress mechanisms and (7) Unassigned and hypothetical genes. CIP (Ciprofloxacin), OXA (Oxacillin), LZD (Linezolid), FOF (Fosfomycin), DAP (Daptomycin), MUP (Mupirocin), VAN (Vancomycin) and GEN (Gentamicin).

Article Snippet: Antimicrobial agents used in the study were erythromycin (Sigma), ciprofloxacin (Sigma), oxacillin (Sigma), linezolid (Sigma), fosfomycin (Sigma), daptomycin (Santa Cruz Biotechnology), mupirocin (Sigma), vancomycin (Sigma) and gentamicin (Sigma).

Techniques: Membrane

Journal: Frontiers in Microbiology

Article Title: Genome-Wide Identification of Antimicrobial Intrinsic Resistance Determinants in Staphylococcus aureus

doi: 10.3389/fmicb.2016.02018

Figure Lengend Snippet: Antimicrobial agents used in the screening for intrinsic resistance determinants, as well as the primary target and process affected by the respective agents.

Article Snippet: Antimicrobial agents used in the study were erythromycin (Sigma), ciprofloxacin (Sigma), oxacillin (Sigma), linezolid (Sigma), fosfomycin (Sigma), daptomycin (Santa Cruz Biotechnology), mupirocin (Sigma), vancomycin (Sigma) and gentamicin (Sigma).

Techniques: Membrane, Permeability