dapi dye (Beyotime)
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Beyotime
dapi dye
Dapi Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 31805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi/DAPI/pmc13123509-556-26-32
Average 99 stars, based on 31805 article reviews
Dapi Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 31805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi/DAPI/pmc13123509-556-26-32
Average 99 stars, based on 31805 article reviews
dapi dye - by Bioz Stars,
2026-09
99/100 stars
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TUNEL Assay:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Staining:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration Article Snippet: The pelleted EVs were pre-labeled with 10 μM PHK26 dye (Beyotime, C2017S) and then co-cultured with NPCs for 12 h, respectively. .. After fixation with 4% paraformaldehyde for 15 min, the Actin-tracker was used for cytoskeleton staining (Beyotime, C2201S), and the nucleus was stained with Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime). .. Cell nuclei were stained with Article Title: Inhalable engineered probiotic outer membrane vesicles co-expressing multiple mpox antigens induce potent specific systemic and mucosal immune responses Article Snippet: MFI and phagocytosis rate were calculated with FlowJo. .. For imaging, cells were fixed, permeabilized, stained with phalloidin (actin, green, BL119A, Biosharp, China) and Article Title: Lung cancer cell-derived exosomal EHF drives M2 macrophage polarization via transcriptional activation of RNF41 to promote tumor progression Article Snippet: .. Nuclei were stained with Incubation:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Microscopy:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Article Title: Prodrug-integrated multifunctional liposome encapsulation enhances probiotic-based bacteriotherapy for inflammatory bowel disease Article Snippet: .. Finally, the slides were coverslipped with an antifade mounting medium containing Article Title: Lung cancer cell-derived exosomal EHF drives M2 macrophage polarization via transcriptional activation of RNF41 to promote tumor progression Article Snippet: .. Nuclei were stained with Laser-Scanning Microscopy:Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury Article Snippet: Cells were then incubated overnight at 4 °C with rabbit polyclonal antibodies against CD86 or CD206 (1:200, Proteintech), followed by 1 h incubation with Alexa Fluor 488-conjugated or CoraLite Plus 594-conjugated goat anti-rabbit IgG secondary antibodies (1:200, Proteintech). .. Nuclei were counterstained with Imaging:Article Title: Inhalable engineered probiotic outer membrane vesicles co-expressing multiple mpox antigens induce potent specific systemic and mucosal immune responses Article Snippet: MFI and phagocytosis rate were calculated with FlowJo. .. For imaging, cells were fixed, permeabilized, stained with phalloidin (actin, green, BL119A, Biosharp, China) and Fluorescence:Article Title: Prodrug-integrated multifunctional liposome encapsulation enhances probiotic-based bacteriotherapy for inflammatory bowel disease Article Snippet: .. Finally, the slides were coverslipped with an antifade mounting medium containing |