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dapi staining reagent  (Servicebio Inc)

 
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    Servicebio Inc dapi staining reagent
    Dapi Staining Reagent, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+reagent/dapi+reagent+staining/pm41697433-173-2-6
    Average 86 stars, based on 1 article reviews
    dapi staining reagent - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Staining:

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia
    Article Snippet: The antibodies and corresponding TSA used in this study are as follows: PKM2 (D78A4) Rabbit mAb and CY3-Tyramide (G1223, Servicebio, China), Anti-CD86 Rabbit mAb and iF488-Tyramide (GB13585, G1231, Servicebio, China), Anti-F4/80 Rabbit pAb and iF647-Tyramide ( GB113373 , G1232, Servicebio, China). .. After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan). .. The activity of pyruvate kinase was measured using pyruvate kinase (PK) activity detection kit (Solarbio, China) according to manufacturer’s instructions.

    Article Title: Ginkgo Biloba extract attenuates diabetic retinopathy progression by modulating TP53 ubiquitination in a rat model
    Article Snippet: The primary antibody against the Gene - RNA Binding Protein, MRNA Processing Factor (RBPMS) (15187-1-AP, proteintech ® , Wuhan, China) was applied to the tissue sections and incubated overnight at 4 °C, followed by incubating the diluted Donkey anti-Rabbit IgG (H + L) (A21207, Invitrogen, USA), a fluorescent secondary antibody, for 1 h at room temperature. .. The slides were incubated in PBS (pH 7.4) with DAPI staining reagent (G1012, Servicebio ® ) for 10 min in the dark. .. Finally, Slices were coverslipped with antifade mounting medium (G1401, Servicebio ® ) and imaged using a laser confocal scanning microscope (LSM880, Zeiss, Germany).

    Article Title: Ginkgo Biloba extract attenuates diabetic retinopathy progression by modulating TP53 ubiquitination in a rat model.
    Article Snippet: The primary antibody against the Gene - RNA Binding Protein, MRNA Processing Factor (RBPMS) (15187-1-AP, proteintech®, Wuhan, China) was applied to the tissue sections and incubated overnight at 4 °C, followed by incubating the diluted Donkey anti-Rabbit IgG (H + L) (A21207, Invitrogen, USA), a fluorescent secondary antibody, for 1 h at room temperature. .. The slides were incubated in PBS (pH 7.4) with DAPI staining reagent (G1012, Servicebio®) for 10 min in the dark. .. Finally, Slices were coverslipped with antifade mounting medium (G1401, Servicebio®) and imaged using a laser confocal scanning microscope (LSM880, Zeiss, Germany).

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Article Title: MXRA7 Alleviates Epididymitis from Exercise-Induced Fatigue by Inhibiting Pyroptosis
    Article Snippet: .. Vitamin C, lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA), BIM 1 (Orileaf, China); Tunel, IL-6, IL-1β, and TNF-α assay kits; 8-OhdG; FITC-labeled Arachis hypogaea agglutinin; Anti-MXRA7 antibody, Anti-p-PKCα (Ser657/Tyr658) Antibody (Sigma-Aldrich); Anti-Hexokinasel antibody; Anti-COX IV antibody; Anti-α-Tubulin antibody; Anti-NLRP3 antibody, Anti-Caspase-1 antibody, Anti-GSDMD antibody, Anti-p-NF-κB p65 antibody, Anti-NF-κB p65 antibody, Anti-p-NF-κB IκBα antibody, Anti-NF-κB IκBα antibody, Anti-NF-κB p52/p100 antibody , Anti-PKCα antibody, Anti-pan-PKC antibody , HRP-conjugated Goat Anti-Rabbit IgG(H+L), (Wuhan Sanying, China); Phos-tag SDS-PAGE (FUJIFILM Wako, Japan); DAPI staining reagent (Wuhan Servicebio, China). ..

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia.
    Article Snippet: The antibodies and corresponding TSA used in this study are as follows: PKM2 (D78A4) Rabbit mAb and CY3-Tyramide (G1223, Servicebio, China), Anti-CD86 Rabbit mAb and iF488Tyramide (GB13585, G1231, Servicebio, China), Anti-F4/80 Rabbit pAb and iF647-Tyramide (GB113373, G1232, Servicebio, China). .. After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan). ..

    Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
    Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.

    Fluorescence:

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia
    Article Snippet: The antibodies and corresponding TSA used in this study are as follows: PKM2 (D78A4) Rabbit mAb and CY3-Tyramide (G1223, Servicebio, China), Anti-CD86 Rabbit mAb and iF488-Tyramide (GB13585, G1231, Servicebio, China), Anti-F4/80 Rabbit pAb and iF647-Tyramide ( GB113373 , G1232, Servicebio, China). .. After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan). .. The activity of pyruvate kinase was measured using pyruvate kinase (PK) activity detection kit (Solarbio, China) according to manufacturer’s instructions.

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia.
    Article Snippet: The antibodies and corresponding TSA used in this study are as follows: PKM2 (D78A4) Rabbit mAb and CY3-Tyramide (G1223, Servicebio, China), Anti-CD86 Rabbit mAb and iF488Tyramide (GB13585, G1231, Servicebio, China), Anti-F4/80 Rabbit pAb and iF647-Tyramide (GB113373, G1232, Servicebio, China). .. After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan). ..

    Microscopy:

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia
    Article Snippet: The antibodies and corresponding TSA used in this study are as follows: PKM2 (D78A4) Rabbit mAb and CY3-Tyramide (G1223, Servicebio, China), Anti-CD86 Rabbit mAb and iF488-Tyramide (GB13585, G1231, Servicebio, China), Anti-F4/80 Rabbit pAb and iF647-Tyramide ( GB113373 , G1232, Servicebio, China). .. After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan). .. The activity of pyruvate kinase was measured using pyruvate kinase (PK) activity detection kit (Solarbio, China) according to manufacturer’s instructions.

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia.
    Article Snippet: The antibodies and corresponding TSA used in this study are as follows: PKM2 (D78A4) Rabbit mAb and CY3-Tyramide (G1223, Servicebio, China), Anti-CD86 Rabbit mAb and iF488Tyramide (GB13585, G1231, Servicebio, China), Anti-F4/80 Rabbit pAb and iF647-Tyramide (GB113373, G1232, Servicebio, China). .. After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan). ..

    Incubation:

    Article Title: Ginkgo Biloba extract attenuates diabetic retinopathy progression by modulating TP53 ubiquitination in a rat model
    Article Snippet: The primary antibody against the Gene - RNA Binding Protein, MRNA Processing Factor (RBPMS) (15187-1-AP, proteintech ® , Wuhan, China) was applied to the tissue sections and incubated overnight at 4 °C, followed by incubating the diluted Donkey anti-Rabbit IgG (H + L) (A21207, Invitrogen, USA), a fluorescent secondary antibody, for 1 h at room temperature. .. The slides were incubated in PBS (pH 7.4) with DAPI staining reagent (G1012, Servicebio ® ) for 10 min in the dark. .. Finally, Slices were coverslipped with antifade mounting medium (G1401, Servicebio ® ) and imaged using a laser confocal scanning microscope (LSM880, Zeiss, Germany).

    Article Title: Ginkgo Biloba extract attenuates diabetic retinopathy progression by modulating TP53 ubiquitination in a rat model.
    Article Snippet: The primary antibody against the Gene - RNA Binding Protein, MRNA Processing Factor (RBPMS) (15187-1-AP, proteintech®, Wuhan, China) was applied to the tissue sections and incubated overnight at 4 °C, followed by incubating the diluted Donkey anti-Rabbit IgG (H + L) (A21207, Invitrogen, USA), a fluorescent secondary antibody, for 1 h at room temperature. .. The slides were incubated in PBS (pH 7.4) with DAPI staining reagent (G1012, Servicebio®) for 10 min in the dark. .. Finally, Slices were coverslipped with antifade mounting medium (G1401, Servicebio®) and imaged using a laser confocal scanning microscope (LSM880, Zeiss, Germany).

    Immunofluorescence:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Western Blot:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
    Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.

    Flow Cytometry:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Purification:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Recombinant:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    TUNEL Assay:

    Article Title: MXRA7 Alleviates Epididymitis from Exercise-Induced Fatigue by Inhibiting Pyroptosis
    Article Snippet: .. Vitamin C, lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA), BIM 1 (Orileaf, China); Tunel, IL-6, IL-1β, and TNF-α assay kits; 8-OhdG; FITC-labeled Arachis hypogaea agglutinin; Anti-MXRA7 antibody, Anti-p-PKCα (Ser657/Tyr658) Antibody (Sigma-Aldrich); Anti-Hexokinasel antibody; Anti-COX IV antibody; Anti-α-Tubulin antibody; Anti-NLRP3 antibody, Anti-Caspase-1 antibody, Anti-GSDMD antibody, Anti-p-NF-κB p65 antibody, Anti-NF-κB p65 antibody, Anti-p-NF-κB IκBα antibody, Anti-NF-κB IκBα antibody, Anti-NF-κB p52/p100 antibody , Anti-PKCα antibody, Anti-pan-PKC antibody , HRP-conjugated Goat Anti-Rabbit IgG(H+L), (Wuhan Sanying, China); Phos-tag SDS-PAGE (FUJIFILM Wako, Japan); DAPI staining reagent (Wuhan Servicebio, China). ..

    SDS Page:

    Article Title: MXRA7 Alleviates Epididymitis from Exercise-Induced Fatigue by Inhibiting Pyroptosis
    Article Snippet: .. Vitamin C, lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA), BIM 1 (Orileaf, China); Tunel, IL-6, IL-1β, and TNF-α assay kits; 8-OhdG; FITC-labeled Arachis hypogaea agglutinin; Anti-MXRA7 antibody, Anti-p-PKCα (Ser657/Tyr658) Antibody (Sigma-Aldrich); Anti-Hexokinasel antibody; Anti-COX IV antibody; Anti-α-Tubulin antibody; Anti-NLRP3 antibody, Anti-Caspase-1 antibody, Anti-GSDMD antibody, Anti-p-NF-κB p65 antibody, Anti-NF-κB p65 antibody, Anti-p-NF-κB IκBα antibody, Anti-NF-κB IκBα antibody, Anti-NF-κB p52/p100 antibody , Anti-PKCα antibody, Anti-pan-PKC antibody , HRP-conjugated Goat Anti-Rabbit IgG(H+L), (Wuhan Sanying, China); Phos-tag SDS-PAGE (FUJIFILM Wako, Japan); DAPI staining reagent (Wuhan Servicebio, China). ..

    cDNA Synthesis:

    Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
    Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.

    Bicinchoninic Acid Protein Assay:

    Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
    Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.

    Real-time Polymerase Chain Reaction:

    Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
    Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.

    SYBR Green Assay:

    Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
    Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.



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    https://www.bioz.com/product/dapi+staining+reagent/dapi+reagent+staining/pm41697433-173-2-6
    Average 86 stars, based on 1 article reviews
    dapi staining reagent - by Bioz Stars, 2026-10
    86/100 stars
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    nuclear staining reagent dapi  (Beyotime)
    99
    Beyotime nuclear staining reagent dapi
    Nuclear Staining Reagent Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+reagent/DAPI/pmc12848013-531-1-5
    Average 99 stars, based on 1 article reviews
    nuclear staining reagent dapi - by Bioz Stars, 2026-10
    99/100 stars
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    dapi staining reagent  (Beyotime)
    99
    Beyotime dapi staining reagent
    Dapi Staining Reagent, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+reagent/DAPI/pm41544528-54-30-39
    Average 99 stars, based on 1 article reviews
    dapi staining reagent - by Bioz Stars, 2026-10
    99/100 stars
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    cell nucleus staining  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc cell nucleus staining
    Cell Nucleus Staining, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+reagent/ProLong+Gold+Antifade+Reagent+with+DAPI/pm41222802-121-15-19
    Average 96 stars, based on 1 article reviews
    cell nucleus staining - by Bioz Stars, 2026-10
    96/100 stars
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