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nuclear counterstaining with dapi  (Beyotime)


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    Structured Review

    Beyotime nuclear counterstaining with dapi
    Nuclear Counterstaining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+nuclear+counterstaining/DAPI/pmc12914690-116-14-18
    Average 99 stars, based on 32539 article reviews
    nuclear counterstaining with dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Spautin-1 inhibits the growth of diffuse large B-cell lymphoma by inducing mitochondrial damage-mediated PANoptosis and anti-tumor immunity.
    Article Snippet: .. DAPI nuclear counterstaining (Beyotime) was followed by multispectral fluorescence imaging (Olympus). .. Paraffin-embedded tumor sections were hydrated with gradient ethanol, accompanied by endogenous peroxidase inactivation and antigen exposure, and stained using an IHC detection kit (Proteintech) according to the manufacturer's instructions.

    Article Title: miR-7480-5p/SIRT3/GSH axis mediates selenium Deficiency-Exacerbated trimethyltin chloride-induced ferroptosis in chicken thymus.
    Article Snippet: The cells/sections were immersed in the following primary antibodies overnight at 4°C (all from ABclonal, China): rabbit anti-SIRT3 (1:200), rabbit antiMAP1LC3B (1:300), rabbit anti-GPX4 (1:300), and rabbit anti-SQSTM1(1:300). .. After washing, sections/cells were incubated with fluorescent secondary antibodies: DyLight 488-conjugated goat anti-rabbit IgG (1:1000, Biodragon, China) or DyLight 594- conjugated goat anti-rabbit IgG (1:1000, Biodragon, China), followed by DAPI nuclear counterstaining (1:450, Beyotime, China) for 10 min. Fluorescence images were captured using a fluorescence microscope (Olympus IX53, Japan) and analyzed with ImageJ software. .. Total iron ion content in the supernatants of thymus tissue homogenates was quantified using a Tissue Iron Assay Kit (A039-2-1; Nanjing Jiancheng Bioengineering Institute, China) strictly following the manufacturer’s protocols.

    Article Title: Spautin-1 inhibits the growth of diffuse large B-cell lymphoma by inducing mitochondrial damage-mediated PANoptosis and anti-tumor immunity
    Article Snippet: .. DAPI nuclear counterstaining (Beyotime) was followed by multispectral fluorescence imaging (Olympus). .. Paraffin-embedded tumor sections were hydrated with gradient ethanol, accompanied by endogenous peroxidase inactivation and antigen exposure, and stained using an IHC detection kit (Proteintech) according to the manufacturer's instructions.

    Article Title: MicroRNA-23b-3p promotes the proliferation, migration, and epithelial-mesenchymal transition of lens epithelial cells by targeting Sprouty2.
    Article Snippet: Cataract, opacification of the lens, is one of the most important reasons of visual impairment and blindness.. Though microRNAs (miRNAs) have been demonstrated to play important roles in cataractogenesis, the underlying molecular mechanisms in this progress remain obscure.. In the present study, microRNA-23b-3p (miR-23b) overexpression promoted the proliferation, migration and epithelial-mesenchymal transition (EMT), whereas miR-23b knockdown markedly inhibited the proliferation, migration and TGF-β-induced EMT of lens epithelial cells (LECs).

    Imaging:

    Article Title: Spautin-1 inhibits the growth of diffuse large B-cell lymphoma by inducing mitochondrial damage-mediated PANoptosis and anti-tumor immunity.
    Article Snippet: .. DAPI nuclear counterstaining (Beyotime) was followed by multispectral fluorescence imaging (Olympus). .. Paraffin-embedded tumor sections were hydrated with gradient ethanol, accompanied by endogenous peroxidase inactivation and antigen exposure, and stained using an IHC detection kit (Proteintech) according to the manufacturer's instructions.

    Article Title: Spautin-1 inhibits the growth of diffuse large B-cell lymphoma by inducing mitochondrial damage-mediated PANoptosis and anti-tumor immunity
    Article Snippet: .. DAPI nuclear counterstaining (Beyotime) was followed by multispectral fluorescence imaging (Olympus). .. Paraffin-embedded tumor sections were hydrated with gradient ethanol, accompanied by endogenous peroxidase inactivation and antigen exposure, and stained using an IHC detection kit (Proteintech) according to the manufacturer's instructions.

    Incubation:

    Article Title: miR-7480-5p/SIRT3/GSH axis mediates selenium Deficiency-Exacerbated trimethyltin chloride-induced ferroptosis in chicken thymus.
    Article Snippet: The cells/sections were immersed in the following primary antibodies overnight at 4°C (all from ABclonal, China): rabbit anti-SIRT3 (1:200), rabbit antiMAP1LC3B (1:300), rabbit anti-GPX4 (1:300), and rabbit anti-SQSTM1(1:300). .. After washing, sections/cells were incubated with fluorescent secondary antibodies: DyLight 488-conjugated goat anti-rabbit IgG (1:1000, Biodragon, China) or DyLight 594- conjugated goat anti-rabbit IgG (1:1000, Biodragon, China), followed by DAPI nuclear counterstaining (1:450, Beyotime, China) for 10 min. Fluorescence images were captured using a fluorescence microscope (Olympus IX53, Japan) and analyzed with ImageJ software. .. Total iron ion content in the supernatants of thymus tissue homogenates was quantified using a Tissue Iron Assay Kit (A039-2-1; Nanjing Jiancheng Bioengineering Institute, China) strictly following the manufacturer’s protocols.

    Article Title: Noncanonical function of sphingosine kinase 2 (SPHK2) sustains hepatic triglyceride homeostasis.
    Article Snippet: Department of Pharmacology, College of Pharmacy; State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD); the Laboratory of Cardiovascular Disease and Molecular Intervention, Harbin Medical University, Harbin, China Guangdong Pharmaceutical University, Guangzhou, China Department of Hepatopancreatobiliary Surgery, Harbin Medical University Cancer Hospital, Harbin, China State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China

    Article Title: MicroRNA-23b-3p promotes the proliferation, migration, and epithelial-mesenchymal transition of lens epithelial cells by targeting Sprouty2.
    Article Snippet: Cataract, opacification of the lens, is one of the most important reasons of visual impairment and blindness.. Though microRNAs (miRNAs) have been demonstrated to play important roles in cataractogenesis, the underlying molecular mechanisms in this progress remain obscure.. In the present study, microRNA-23b-3p (miR-23b) overexpression promoted the proliferation, migration and epithelial-mesenchymal transition (EMT), whereas miR-23b knockdown markedly inhibited the proliferation, migration and TGF-β-induced EMT of lens epithelial cells (LECs).

    Microscopy:

    Article Title: miR-7480-5p/SIRT3/GSH axis mediates selenium Deficiency-Exacerbated trimethyltin chloride-induced ferroptosis in chicken thymus.
    Article Snippet: The cells/sections were immersed in the following primary antibodies overnight at 4°C (all from ABclonal, China): rabbit anti-SIRT3 (1:200), rabbit antiMAP1LC3B (1:300), rabbit anti-GPX4 (1:300), and rabbit anti-SQSTM1(1:300). .. After washing, sections/cells were incubated with fluorescent secondary antibodies: DyLight 488-conjugated goat anti-rabbit IgG (1:1000, Biodragon, China) or DyLight 594- conjugated goat anti-rabbit IgG (1:1000, Biodragon, China), followed by DAPI nuclear counterstaining (1:450, Beyotime, China) for 10 min. Fluorescence images were captured using a fluorescence microscope (Olympus IX53, Japan) and analyzed with ImageJ software. .. Total iron ion content in the supernatants of thymus tissue homogenates was quantified using a Tissue Iron Assay Kit (A039-2-1; Nanjing Jiancheng Bioengineering Institute, China) strictly following the manufacturer’s protocols.

    Article Title: MicroRNA-23b-3p promotes the proliferation, migration, and epithelial-mesenchymal transition of lens epithelial cells by targeting Sprouty2.
    Article Snippet: Cataract, opacification of the lens, is one of the most important reasons of visual impairment and blindness.. Though microRNAs (miRNAs) have been demonstrated to play important roles in cataractogenesis, the underlying molecular mechanisms in this progress remain obscure.. In the present study, microRNA-23b-3p (miR-23b) overexpression promoted the proliferation, migration and epithelial-mesenchymal transition (EMT), whereas miR-23b knockdown markedly inhibited the proliferation, migration and TGF-β-induced EMT of lens epithelial cells (LECs).



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    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    Image Search Results


    (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Journal: Bioengineering & Translational Medicine

    Article Title: Extracellular matrix microarchitecture modulates cellular behavior and extracellular vesicle phenotypes in biomimetic tendon models

    doi: 10.1002/btm2.70134

    Figure Lengend Snippet: (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Article Snippet: Cellular morphology was assessed via actin staining (Invitrogen, Cat. No. A12379) and nuclear counterstaining with DAPI (Fisher Scientific, Cat. No. H‐1200‐10) ( n = 3).

    Techniques: Staining, Derivative Assay, Control