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mouse embryonic stem cells cell mes lines d3  (ATCC)


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    ATCC mouse embryonic stem cells cell mes lines d3
    Mouse Embryonic Stem Cells Cell Mes Lines D3, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d3+mes+cells/ES-D3%3B+Embryonic+Stem+Cell%3B+Mouse/pm36936789-218-18-26
    Average 91 stars, based on 1 article reviews
    mouse embryonic stem cells cell mes lines d3 - by Bioz Stars, 2026-10
    91/100 stars

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    Cell Culture:

    Article Title: STAT3-Dependent Mouse Embryonic Stem Cell Differentiation Into Cardiomyocytes
    Article Snippet: .. The D3-mES cells were obtained from ATCC and cultured in complete DMEM medium as described earlier.10 .. The murine recombinant LIF and anti-LIF were purchased from Chemicon International (Temecula, Calif), human BMP-2 was purchased from PeproTech (Rocky Hill, NJ), Cucurbitacin I (JAK-STAT inhibitor) and U0126 (MAPK inhibitor) were purchased from Calbiochem (La Jolla, Calif).

    Article Title: Paraquat toxicity in a mouse embryonic stem cell model.
    Article Snippet: The objective of this in vitro study was to use a mouse embryonic stem (mES) cell model to better understand pesticide injury that may adversely affect early pregnancy and to evaluate an antioxidant intervention.. Undifferentiated D3 mES cells were incubated 24 h with control, reference dose (RfD), no observed effect level (NOEL), or lowest observed effect level (LOEL) of paraquat, a commonly used, toxic agricultural herbicide.. Pesticide effects were evaluated at 0 and 24 h using assays for cell proliferation, total reactive oxygen species (ROS), viability, and alkaline phosphatase activity.

    Article Title: BRG1 interacts with GLI2 and binds Mef2c gene in a hedgehog signalling dependent manner during in vitro cardiomyogenesis
    Article Snippet: .. D3 mES cells (ATCC, #CRL-1934) were cultured with 10 % fetal bovine serum (FBS, Wisent Inc.) and leukemia inhibitory factor (LIF, Millipore). .. D3 cells were stably transfected with the empty pcDNA3.1+ vector or the pcDNA3.1+ vector expressing Flag-Gli2 S662A , a full-length complementary DNA of mouse Gli2 driven by the CMV promoter, in frame with the Flag epitope and containing the serine to alanine mutation at position 662 which prevents phosphorylation and proteasomal degradation [ ].

    Knock-Out:

    Article Title: Simple, efficient, and reproducible gene transfection of mouse embryonic stem cells by magnetofection.
    Article Snippet: Embryonic stem (ES) cells are recognized as an excellent cell culture model for studying developmental mechanisms and their therapeutic modulations.. The aim of this work was to define whether using magnetofection was an efficient way to manipulate stem cells genetically without adversely affecting their proliferation or self-renewal capacity.. We compared our magnetofection results to those of a conservative method using FuGENE 6.



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    RTqPCR quantification of gene marker expression in the differentiated neuron-like cells (a). OCT4 expression in <t>undifferentiated</t> <t>mES</t> cells and neuron-like cells differentiated from mES cells. <t>D3:</t> Undifferentiated condition, statoacoustic ganglion: (SAG) spiral ganglion neuron culture (control). (b). Expression of proneural marker genes in neuron-like cells cells. Ngn1, NeuroD and Nfil expression is much higher in neuronally differentiated cells, but not detected in undifferentiated condition (D3ES). (c). Expression of mature neuronal marker genes: TrkB, TrkC, Prph in the neuron-like cells are upregulated in all the neuronal differentiated conditions and expression levels are much higher under conditions with DHA addition. F12: Neuronal basal media, C: CNTF differentiation condition, N: NGF differentiation condition, M: MIF differentiation condition, D: DHA differentiation condition, CD: CNTF+DHA differentiation condition, ND: NGF+DHA differentiation condition, MD: MIF+DHA differentiation condition. (d). Expression of auditory neuronal markers, GabaAR, VgluT1 and SP19 are highly upregulated in the neuronal differentiation conditions compared to undifferentiated conditions and F12 (basal) medium. VgluT1 expression is much higher in the MIF+DHA condition than all the other differentiation conditions, which was to be expected, given that MIF is the inner ear’s first differentiation “neurotrophin” (Holmes et al., 2011; Bank et al. 2012; Shen et al., 2012).
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    RTqPCR quantification of gene marker expression in the differentiated neuron-like cells (a). OCT4 expression in undifferentiated mES cells and neuron-like cells differentiated from mES cells. D3: Undifferentiated condition, statoacoustic ganglion: (SAG) spiral ganglion neuron culture (control). (b). Expression of proneural marker genes in neuron-like cells cells. Ngn1, NeuroD and Nfil expression is much higher in neuronally differentiated cells, but not detected in undifferentiated condition (D3ES). (c). Expression of mature neuronal marker genes: TrkB, TrkC, Prph in the neuron-like cells are upregulated in all the neuronal differentiated conditions and expression levels are much higher under conditions with DHA addition. F12: Neuronal basal media, C: CNTF differentiation condition, N: NGF differentiation condition, M: MIF differentiation condition, D: DHA differentiation condition, CD: CNTF+DHA differentiation condition, ND: NGF+DHA differentiation condition, MD: MIF+DHA differentiation condition. (d). Expression of auditory neuronal markers, GabaAR, VgluT1 and SP19 are highly upregulated in the neuronal differentiation conditions compared to undifferentiated conditions and F12 (basal) medium. VgluT1 expression is much higher in the MIF+DHA condition than all the other differentiation conditions, which was to be expected, given that MIF is the inner ear’s first differentiation “neurotrophin” (Holmes et al., 2011; Bank et al. 2012; Shen et al., 2012).

    Journal: Developmental dynamics : an official publication of the American Association of Anatomists

    Article Title: Concomitant differentiation of a population of mouse embryonic stem cells into neuron-like cells and Schwann cell-like cells in a slow-flow microfluidic device

    doi: 10.1002/dvdy.24466

    Figure Lengend Snippet: RTqPCR quantification of gene marker expression in the differentiated neuron-like cells (a). OCT4 expression in undifferentiated mES cells and neuron-like cells differentiated from mES cells. D3: Undifferentiated condition, statoacoustic ganglion: (SAG) spiral ganglion neuron culture (control). (b). Expression of proneural marker genes in neuron-like cells cells. Ngn1, NeuroD and Nfil expression is much higher in neuronally differentiated cells, but not detected in undifferentiated condition (D3ES). (c). Expression of mature neuronal marker genes: TrkB, TrkC, Prph in the neuron-like cells are upregulated in all the neuronal differentiated conditions and expression levels are much higher under conditions with DHA addition. F12: Neuronal basal media, C: CNTF differentiation condition, N: NGF differentiation condition, M: MIF differentiation condition, D: DHA differentiation condition, CD: CNTF+DHA differentiation condition, ND: NGF+DHA differentiation condition, MD: MIF+DHA differentiation condition. (d). Expression of auditory neuronal markers, GabaAR, VgluT1 and SP19 are highly upregulated in the neuronal differentiation conditions compared to undifferentiated conditions and F12 (basal) medium. VgluT1 expression is much higher in the MIF+DHA condition than all the other differentiation conditions, which was to be expected, given that MIF is the inner ear’s first differentiation “neurotrophin” (Holmes et al., 2011; Bank et al. 2012; Shen et al., 2012).

    Article Snippet: Culturing mES cells Cell lines: The cell line used for these studies was the D3 mES cell line (ATCC-CRL-1934; Doetschman et al., 1985 ).

    Techniques: Marker, Expressing, Control