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d1r agonist skf 38393 hydrochloride  (MedChemExpress)
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
D1r Agonist Skf 38393 Hydrochloride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d1r agonist skf  (MedChemExpress)
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
D1r Agonist Skf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
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d1r antagonist sch23390  (MedChemExpress)
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
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d1r gαs ro7305235  (SPT Labtech)
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
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d1r gαs ro7295973  (SPT Labtech)
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of <t>D1R</t> in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).
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( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of D1R in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).

Journal: The Journal of Clinical Investigation

Article Title: A midbrain–cortical circuit mediated by a claustrum neuronal ensemble orchestrates drug-paired context memory processing

doi: 10.1172/JCI196944

Figure Lengend Snippet: ( A ) Experimental design. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and fiber implant in CL. ( D ) Heatmap of DA3h fluorescence (left), quantification (middle), and AUC (right) of ΔF/F in CL. n = 5 mice/group. ( E ) The protein levels of D1R in CL. The protein levels of D1R following the METH CPP-Test. n = 3 mice/group. ( F ) Immunofluorescence of c-Fos + CaMKII + D1R + neurons in CL. Mean gray value of D1R CaMKII following METH CPP-Test. n = 6 mice/group. * P < 0.05. Two-tailed unpaired t test ( D – F ). Scale bars: 100 μm ( C and F ).

Article Snippet: The DA hydrochloride (ASL279, MedChemExpress) or D1R agonist (±)-SKF-38393 hydrochloride (SKF-38393, MedChemExpress) was diluted to 10 mM in 0.9% saline by shaking.

Techniques: Transfection, Virus, Injection, Fluorescence, Immunofluorescence, Two Tailed Test

( A ) Experimental design and timeline. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and cannula implantation in VTA or CL. ( D ) Analysis of CPP behavior of time spent in CPP apparatus. S-CNO+Veh/S-CNO+DA group, n = 6 mice/group; M-CNO+Veh/M-CNO+DA group, n = 8 mice/group. ( E ) Representative heatmap of time spent in CPP apparatus during CPP-Test. ( F ) Experimental design and timeline. ( G ) Schematic of viral transfection. ( H ) Representative images of virus injection and cannula implantation in VTA or CL. ( I ) Analysis of CPP behavior of time spent in CPP apparatus. S-CNO+Veh/S-CNO+SKF group, n = 5 mice/group; M-CNO+Veh/M-CNO+SKF group, n = 6 mice/group. ( J ) Representative heatmap of time spent in CPP apparatus during CPP-Test. ( K ) Experimental design and timeline. ( L ) Schematic of viral transfection. ( M ) Representative images of virus injection in CL. ( N ) Analysis of CPP behavior and heatmap of time spent in CPP apparatus. S-CTRL/S-KD group, n = 6 mice/group; M-CTRL/M-KD group, n = 8 mice/group. ( O ) Representative heatmap of time spent in CPP apparatus during CPP-Test. ( P ) Immunofluorescence of c-Fos + D1R + EGFP + neurons in CL following CPP-Test. The percentage of c-Fos + EGFP + neurons in EGFP + neurons of CL. n = 6 mice/group. NS, P > 0.05, * P < 0.05, ** P < 0.01. Two-way ANOVA with Šidák’s multiple-comparison test ( D , I , N , and P ). Scale bars: 100 μm ( C , H , M , and P ).

Journal: The Journal of Clinical Investigation

Article Title: A midbrain–cortical circuit mediated by a claustrum neuronal ensemble orchestrates drug-paired context memory processing

doi: 10.1172/JCI196944

Figure Lengend Snippet: ( A ) Experimental design and timeline. ( B ) Schematic of viral transfection. ( C ) Representative images of virus injection and cannula implantation in VTA or CL. ( D ) Analysis of CPP behavior of time spent in CPP apparatus. S-CNO+Veh/S-CNO+DA group, n = 6 mice/group; M-CNO+Veh/M-CNO+DA group, n = 8 mice/group. ( E ) Representative heatmap of time spent in CPP apparatus during CPP-Test. ( F ) Experimental design and timeline. ( G ) Schematic of viral transfection. ( H ) Representative images of virus injection and cannula implantation in VTA or CL. ( I ) Analysis of CPP behavior of time spent in CPP apparatus. S-CNO+Veh/S-CNO+SKF group, n = 5 mice/group; M-CNO+Veh/M-CNO+SKF group, n = 6 mice/group. ( J ) Representative heatmap of time spent in CPP apparatus during CPP-Test. ( K ) Experimental design and timeline. ( L ) Schematic of viral transfection. ( M ) Representative images of virus injection in CL. ( N ) Analysis of CPP behavior and heatmap of time spent in CPP apparatus. S-CTRL/S-KD group, n = 6 mice/group; M-CTRL/M-KD group, n = 8 mice/group. ( O ) Representative heatmap of time spent in CPP apparatus during CPP-Test. ( P ) Immunofluorescence of c-Fos + D1R + EGFP + neurons in CL following CPP-Test. The percentage of c-Fos + EGFP + neurons in EGFP + neurons of CL. n = 6 mice/group. NS, P > 0.05, * P < 0.05, ** P < 0.01. Two-way ANOVA with Šidák’s multiple-comparison test ( D , I , N , and P ). Scale bars: 100 μm ( C , H , M , and P ).

Article Snippet: The DA hydrochloride (ASL279, MedChemExpress) or D1R agonist (±)-SKF-38393 hydrochloride (SKF-38393, MedChemExpress) was diluted to 10 mM in 0.9% saline by shaking.

Techniques: Transfection, Virus, Injection, Immunofluorescence, Comparison