Journal: Brain, behavior, and immunity

Article Title: HIV-1 Tat protein enhances sensitization to methamphetamine by affecting dopaminergic function

doi: 10.1016/j.bbi.2017.05.004

Figure Lengend Snippet: Immunohistochemistry on paraffin embedded sections was utilized examine the protein distribution and levels of dopamine receptor D1 (A, B, C, D), dopamine receptor D2 (E, F, G, H), as well as of IBA-1 (I, J, K, L) in SAL TAT− (A, E, I), SAL TAT+ (B, F, J), METH TAT− (C, G, K), and METH TAT+ (D, H, L) mice. Representative positive cells in the 40× magnification images were labeled with a black arrow. (M) Normalized intensity density was calculated in ImageJ. Data are expressed as Mean ± SEM (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Sections were blocked with 5g/l Casein (Sigma Aldrich) in PBS, containing 0.5g/l Thimerosal (Sigma Aldrich) and incubated with Iba-1 antibody (Wako Lab Chemicals, Richmond, VA), the anti-mouse DRD1 antibody (NLS43, Novus Biologicals, Littleton, CO), or anti mouse DRD2 (orb154598, Biorbyt, San Francisco, CA), each one diluted in Casein buffer.

Techniques: Immunohistochemistry, Labeling

Journal: Translational Cancer Research

Article Title: DRD1 and DRD4 are differentially expressed in breast tumors and breast cancer stem cells: pharmacological implications

doi: 10.21037/tcr-22-783

Figure Lengend Snippet: Expression of DRs breast cancer cell lines. (A) mRNA expression of DRDs in 62 breast cancer cell lines form the Cancer Cell Line Encyclopedia. MCF-7 and MDA-MB-231 cell lines are shown in magenta and red, respectively. (B,C) Protein quantification of DRD1, DRD2, and DRD4 in MCF-7 (B) and MDA-MB-231 (C) by flow cytometry. Graphs in (B) and (C) show MFI (average ± SEM) from 2–3 independent experiments. **, P<0.05 (Student’s t- test). DR, dopamine receptor; MFI, mean fluorescence intensity; SEM, standard error of the mean; TPM, transcript per million.

Article Snippet: Expression of DRs was analyzed using anti-human DRD1 Alexa Fluor 405 (FAB8276V, R&D Systems), anti-human DRD2 Alexa Fluor 647 (sc-5303, Santa Cruz Biotechnology), or anti-human DRD4 PE (sc-136169, Santa Cruz Biotechnology).

Techniques: Expressing, Flow Cytometry, Fluorescence

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Interplay between coronavirus, a cytoplasmic RNA virus, and nonsense-mediated mRNA decay pathway

doi: 10.1073/pnas.1811675115

Figure Lengend Snippet: Cytoplasmically synthesized rLucRNA3, but not GLA mRNA, is susceptible to NMD. ( A ) UPF1-depleted 17Cl-1 cells (siUpf1), UPF2-depleted 17Cl-1 cells (siUpf2), or 17Cl-1 cells treated with control siRNAs (siCtrl) were cotransfected with plasmids expressing T7 polymerase, rLucRNA3, vaccinia virus capping enzyme D1R, and vaccinia virus capping enzyme D12L. At 20 h after plasmid transfection, cells were treated with actD, and levels of expressed rLucRNA3 at 0, 2, and 4 h after actD treatment were determined by qRT-PCR and normalized to 18S rRNA levels. The data represent the mean with SEM of three independent experiments. Statistical analysis was done by ANOVA. ** P < 0.01. ( B ) Experiments were performed as described in A , except that plasmid encoding GLA RNA was used in place of that encoding rLucRNA3. ( C ) The 17Cl-1 cells were cotransfected as described in A . At 1 h after actD addition, DMSO, CHX, or wortmannin (Wort) was added to the cells, and levels of rLucRNA3 at 1 and 3 h after actD addition were determined by qRT-PCR and normalized to 18S rRNA levels. The data represent the mean with SEM of at least three independent experiments. Statistical analysis was done by ANOVA. * P < 0.05.

Article Snippet: The 17Cl-1 cells were cotransfected with plasmids encoding either T7-rLucRNA3 plasmid or T7-GLA plasmid, along with plasmids encoding T7 polymerase, T7opt , vaccinia virus capping enzyme D1R ( ) (89160; Addgene), or vaccinia virus capping enzyme D12L ( ) (89161; Addgene).

Techniques: Synthesized, Control, Expressing, Virus, Plasmid Preparation, Transfection, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Microglia-mediated synaptic pruning in the nucleus accumbens during adolescence: A preliminary study of the proteomic consequences and putative female-specific pruning target

doi: 10.1101/2023.05.02.539121

Figure Lengend Snippet: In remaining tissue (unpooled) that underwent proteomic analysis ( , ) we conducted ELISAs to validate potential female-specific pruning targets. (A) Inhibiting pruning in the NAc significantly decreased Lynx1 expression in females, but not males. n =7–8/sex/condition. (B) The Lynx1 target, NAChR, was not regulated by NIF treatment in either sex. n =7–8/sex/condition. (C) In remaining, but underpowered sample sizes ( n =3–5/sex/condition), we observed the expected increase in D1r levels after inhibiting NAc pruning in males, but not females. This is consistent with our previously published results. In each histogram, horizontal lines are average and vertical lines are standard error of the mean. * p <0.05.

Article Snippet: Lynx1 ELISA was purchased from mybiosource.com (#MBS9902329), NAChR ELISA was purchased from LSBio (#LS-F37436), and D1r ELISA was purchased from Novus Biologicals (NBP2–67935).

Techniques: Expressing