ca2 sensor pcdna d1er (Addgene inc)

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Addgene inc ca2 sensor pcdna d1er
Ca2 Sensor Pcdna D1er, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d1er/pcDNA-D1ER+(Plasmid+%2336325)/pm41316250-125-23-28
Average 93 stars, based on 45 article reviews

ca2 sensor pcdna d1er - by Bioz Stars, 2026-09

93/100 stars

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Transfection:

Article Title: IK Ca channels control breast cancer metabolism including AMPK-driven autophagy.
Article Snippet: Polyjet-transfection protocol (SignaGen laboratories, Ballenger Creek, USA) was carried out according to the manufacturer’s instructions for 16 h overnight resulting in a reproducible expression level of the FRET sensors used (General Protocol for Transfecting Mammalian Cell with PolyJetTM In Vitro DNA Transfection Reagent). .. Following plasmids were provided by Addgene (Watertown, USA) and transfected with Polyjet: 4mtD3cpv (Addgene #58184), AKTAR (Addgene #61624), AMPKAR (Addgene #35097), CARGeco1 (Addgene #45493), cyto lc-LysM GEPII 1.0, D1ER (Addgene #36325), Laconic (Addgene #44238), mito lc-LysM GEPII 1.0, mtAT1.03, TORCAR, NesAT1.03, pH-Lemon – LC3B and Pyronic (Addgene #51308). ..

Article Title: SERCA2 phosphorylation at serine 663 is a key regulator of Ca 2+ homeostasis in heart diseases
Article Snippet: Drugs were added to buffers for stimulations [ATP-Na 2 (Sigma, A7699); ATP-Mg 2+ (Sigma, A9187); 1 μM ionomycin (Abcam, ab-120370); 100 μM cyclopiazonic acid (CPA, Sigma, C1530); 1 μM thapsigargin (TG, Sigma, T9033); 1 μM SB216763 (Sigma, S3442); 10 μM TDZD8 (Sigma, T8325); 5 mM caffeine (Sigma, C0750)]. .. Genetically encoded Ca 2+ probes D3cpV (Addgene #36323) , 4mtD3cpV (Addgene #36324) and D1ER (Addgene #36325) were transiently transfected into cells 48 h before monitoring Ca 2+ levels and dynamics in different cellular compartments, thanks to their FRET-based ratiometric properties. .. Chemical intracellular Ca 2+ indicators, ratiometric Fura-2 AM (Invitrogen, F1221) or non-ratiometric Fluo-4 AM (Invitrogen, F14201), were loaded into the cells by incubating 5 μM for 30 minutes at 20 °C or 37 °C respectively.

Article Title: CDN1163, an activator of sarco/endoplasmic reticulum Ca 2+ ATPase, up-regulates mitochondrial functions and protects against lipotoxicity in pancreatic β-cells.
Article Snippet: .. For ER Ca2+ measurements, either G-CEPIA1er (RRID: Addgene_105012) or D1ER (RRID:Addgene_36325) was transfected 48 h before the experiment. .. The mitochondrial matrix Ca2+ level was measured after transient transfection of either the CEPIA-2mt (RRID:Addgene_58218) or mtD3cpv (RRID:Addgene_ 58184) carrying plasmid.

Article Title: SERCA2 phosphorylation at serine 663 is a key regulator of Ca 2+ homeostasis in heart diseases.
Article Snippet: .. Genetically encoded Ca2+ probes D3cpV (Addgene #36323)62, 4mtD3cpV (Addgene #36324)62 and D1ER (Addgene #36325)63 were transiently transfected into cells 48 h before monitoringCa2+ levels anddynamics indifferent cellular compartments, thanks to their FRET-based ratiometric properties. .. Chemical intracellular Ca2+ indicators, ratiometric Fura-2 AM (Invitrogen, F1221) or nonratiometric Fluo-4 AM (Invitrogen, F14201), were loaded into the cells by incubating 5 μM for 30minutes at 20 °C or 37 °C respectively.

Plasmid Preparation:

Article Title: mitoBK Ca is functionally expressed in murine and human breast cancer cells and promotes metabolic reprogramming
Article Snippet: .. Sensor plasmids used in this study comprised the following: D1ER ( ) (Addgene plasmid #36325) for measurement of [Ca 2+ ] ER and 4mtD3cpV ( ) (Addgene plasmid #36324) for measurement of [Ca 2+ ] mito were a gift from Amy Palmer & Roger Tsien, mtAT1.03 ( ) for measurement of [ATP] mito was a gift from Hiromi Imamura, Laconic (San Martín et al., 2013) (Addgene plasmid #44238) for measurement of [LAC] cyto and assessment of the Warburg index was a gift from Luis Felipe Barros, and mito-Hyper3 ( ) was a gift from Markus Waldeck-Weiermair. .. Experiments were either performed on the AXIO Observer Z1 or the Axiovert 200m microscope (Carl Zeiss AG).

Article Title: mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
Article Snippet: .. Sensor plasmids used in this study comprised the following: D1ER (Palmer et al., 2004; Addgene plasmid #36325) for measurement of [Ca2+]ER and 4mtD3cpV (Palmer et al., 2006) (Addgene plasmid #36324) for measurement of [Ca2+]mito were a gift from Amy Palmer & Roger Tsien, mtAT1.03 (Imamura et al., 2009) for measurement of [ATP]mito was a gift from Hiromi Imamura, Laconic (San Martín et al., 2013) (Addgene plasmid #44238) for measurement of [LAC]cyto and assessment of the Warburg index was a gift from Luis Felipe Barros, mito- Hyper3 (Bilan et al., 2013) was a gift from Markus Waldeck- Weiermair and NES lc- LysM GEPII 1.0 (Bischof et al., 2017) was a gift from Roland Malli. .. Experiments were either performed on the AXIO Observer Z1 or the Axiovert 200M microscope (Carl Zeiss AG).

Article Title: mitoBK Ca is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
Article Snippet: .. Sensor plasmids used in this study comprised the following: D1ER ( ; Addgene plasmid #36325) for measurement of [Ca 2+ ] ER and 4mtD3cpV ( ) (Addgene plasmid #36324) for measurement of [Ca 2+ ] mito were a gift from Amy Palmer & Roger Tsien, mtAT1.03 ( ) for measurement of [ATP] mito was a gift from Hiromi Imamura, Laconic ( ) (Addgene plasmid #44238) for measurement of [LAC] cyto and assessment of the Warburg index was a gift from Luis Felipe Barros, mito-Hyper3 ( ) was a gift from Markus Waldeck-Weiermair and NES lc-LysM GEPII 1.0 ( ) was a gift from Roland Malli. .. Experiments were either performed on the AXIO Observer Z1 or the Axiovert 200M microscope (Carl Zeiss AG).

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