protein expression  (R&D Systems)

Journal: Osteoarthritis and cartilage

Article Title: Osteoarthritic synovial fibroblasts drive inflammatory responses and impair chondrocyte function in response to a fibronectin matrikine

doi: 10.1016/j.joca.2026.04.011

Figure Lengend Snippet: FN7–10-induced changes in synovial fibroblast pro-inflammatory protein secretion. (A) Schematic timeline showing synovial fibroblast serum starvation, stimulation with 1 μM FN7–10, and conditioned media collection for cytokine array analysis (created with BioRender). (B) Representative cytokine arrrays incubated with conditioned media (CM) from synovial fibroblast treated with PBS (left) or FN7–10 (right). Red arrows highlight cytokines detected in the control condition (PBS CM) and increased by at least 1.5-log2fold following FN7–10 stimulation. Positive controls for the array are labeled “POS”. (C) Normalized densitometric quantification of selected cytokines from PBS CM, showing individual donor values with mean ± 95% confidence intervals (n = 4 independent synovial fibroblast donors). (D) Densitometry-based log 2 fold change of selected cytokines detected in the array comparing FN7–10 CM to PBS CM, displayed as individual donor values with mean ± 95% confidence intervals.

Article Snippet: The C5 human cytokine protein array (RayBiotech, AAH-CYT-5–2) was used to evaluate the secreted proteins found in the CM.

Techniques: Incubation, Control, Labeling

Journal: Osteoarthritis and cartilage

Article Title: Osteoarthritic synovial fibroblasts drive inflammatory responses and impair chondrocyte function in response to a fibronectin matrikine

doi: 10.1016/j.joca.2026.04.011

Figure Lengend Snippet: FN710–induced changes in synovial fibroblast cytokine and chemokine gene expression. (A) Boxplot showing the log 2 fold change of significantly regulated genes (padj < 0.05) within the KEGG cytokine–cytokine receptor interaction pathway (hsa04060), grouped by major cytokine families. Chemokine datapoints with |log 2 FC= > 4 are bolded and labeled in black, and the horizontal dashed line indicates no change (log 2 FC = 0). (B) Targeted heatmap illustrating expression of chemokine family genes identified in panel A, comparing synovial fibroblasts treated with PBS or FN710 for 18 h (n = 5 independent OA synovial fibroblast donors). Values represent relative RNA expression (FN7–10/PBS).

Article Snippet: The C5 human cytokine protein array (RayBiotech, AAH-CYT-5–2) was used to evaluate the secreted proteins found in the CM.

Techniques: Gene Expression, Labeling, Expressing, RNA Expression

Journal: Science advances

Article Title: Caspase-1-dependent pyroptosis converts αSMA + CAFs into collagen-III high iCAFs to fuel chemoresistant cancer stem cells.

doi: 10.1126/sciadv.adt8697

Figure Lengend Snippet: Fig. 4. Tumor cell pyroptosis skews CAFs toward iCAFs in a CCR6-dependent mechanism. (A) Representative image illustrating a cytokine protein array (i.e., Proteome Profiler Human XL Cytokine Array Kit, R&D Systems) probed with the supernatant collected from WT and Casp1 KO T24 cancer cells after 48 hours of gemcitabine treat- ment. Red, blue, and green boxes highlighting the individual cytokines or chemokines with the most differential expression (i.e., reduction) in Casp1 KO versus WT Gem- treated supernatant. (B) Bar graph quantifying the intensity of three cytokines differentially released in WT and Casp1 KO supernatant, i.e., CXCL5, CXCL10, and CCL20. (C) ELISA quantification of CXCL5, CXCL10, and CCL20 protein concentration in the supernatants collected from gemcitabine-treated WT and Casp1 KO T24 blad- der cancer cells. (D) Flow cytometry assessing the percentage of αSMAhigh/+ CAFs and αSMA−PDGFβ+ iCAFs treated with CM from Gem-treated T24 cells with anti-CXCR2 neutralizing Ab, (anti-CXCR2 Ab, blocking receptor downstream to CXCL5), anti-CXCL10 neutralizing Ab, anti-CCL20 neutralizing ab, and CCR6i (blocking receptor down- stream to CCL20). (E) Violin dot plot quantifying the relative changes in the percentage of αSMA−PDGFβ+ iCAFs upon CM-Gem ± chemokine or chemokine receptor neutralizing Ab treatments.

Article Snippet: Tumor cell pyroptosis skews CAFs toward iCAFs in a CCR6- dependent mechanism. (A) Representative image illustrating a cytokine protein array (i.e., Proteome Profiler human Xl cytokine Array Kit, R&d Systems) probed with the supernatant collected from Wt and casp1 KO t24 cancer cells after 48 hours of gemcitabine treatment.

Techniques: Protein Array, Quantitative Proteomics, Enzyme-linked Immunosorbent Assay, Protein Concentration, Flow Cytometry, Blocking Assay