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cytokine array membrane  (R&D Systems)


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    R&D Systems cytokine array membrane
    Cytokine Array Membrane, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 748 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytokine+array+membrane/Proteome+Profiler+Mouse+Cytokine+Array+Kit%2C+Panel+A/pm41916428-100-31-34
    Average 96 stars, based on 748 article reviews
    cytokine array membrane - by Bioz Stars, 2026-09
    96/100 stars

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    Membrane:

    Article Title: Basal phosphorylation of SHIP1 by Lyn suppresses proinflammatory signaling in the absence of a phagocytic synapse.
    Article Snippet: .. IFN-γ-primed BMDMs were treated 8 h with 10 μM 3-IB-PP1 or 10:1 depleted zymosan, and supernatants were centrifuged 5 min at 311 g to remove cell debris and applied to a cytokine-array membrane (ARY006; R&D Systems). .. Membranes were visualized using an Odyssey CLx nearinfrared imager following incubation with IRDye 800CW Streptavidin secondary (NC9386176; LiCor).

    Article Title: Basal phosphorylation of SHIP1 by Lyn suppresses proinflammatory signaling in the absence of a phagocytic synapse
    Article Snippet: .. IFN-γ-primed BMDMs were treated 8 h with 10 μM 3-IB-PP1 or 10:1 depleted zymosan, and supernatants were centrifuged 5 min at 311 g to remove cell debris and applied to a cytokine-array membrane (ARY006; R&D Systems). .. Membranes were visualized using an Odyssey CLx near-infrared imager following incubation with IRDye 800CW Streptavidin secondary (NC9386176; LiCor).



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    Benzo-17R-RvD2 mimetic in peritonitis limits PMN tissue infiltration and regulates cytokines Mice were administered with vehicle or benzo-17R-RvD2 (1 ng/mouse) together with zymosan A (1 mg/mouse, i.p.) and exudates collected at 4 h. Total leukocytes were enumerated and composition determined by flow cytometry (see B for gating strategy). (A) Exudate PMN numbers; mean ± SEM, n = 4 mice each group, ∗∗ p < 0.01. (B) Proteome analysis was carried out with inflammatory exudates from four individual mice in each group using Proteome Profiler cytokine arrays, which contains 40 cytokines, chemokines, and acute phase proteins. Orthogonal Partial Least Squares Discriminant Analysis (PLS-DA): the score plot (each dot represents the cytokine profile from each mouse) shows clustering among groups (i.e., peritonitis vs. peritonitis plus benzo-17R-RvD2). The loading plot demonstrates correlations in which the measured cytokines contribute to the cluster separation in the score plot. (C) Relative intensities of cytokines, chemokines, and acute phase proteins. Proteome arrays consist of 40 proteins. Among them 18 were present in the peritoneal exudates. Results are mean ± SEM; relative abundance from n = 4 individual mouse exudates, ∗ p < 0.05, ∗∗ p < 0.01; peritonitis plus benzo-17R-RvD2 versus peritonitis alone; Student’s t test.

    Journal: iScience

    Article Title: Nouveau benzo-mimetics of 17R-Resolvin D2 are potent resolution agonists for inflammation

    doi: 10.1016/j.isci.2025.114201

    Figure Lengend Snippet: Benzo-17R-RvD2 mimetic in peritonitis limits PMN tissue infiltration and regulates cytokines Mice were administered with vehicle or benzo-17R-RvD2 (1 ng/mouse) together with zymosan A (1 mg/mouse, i.p.) and exudates collected at 4 h. Total leukocytes were enumerated and composition determined by flow cytometry (see B for gating strategy). (A) Exudate PMN numbers; mean ± SEM, n = 4 mice each group, ∗∗ p < 0.01. (B) Proteome analysis was carried out with inflammatory exudates from four individual mice in each group using Proteome Profiler cytokine arrays, which contains 40 cytokines, chemokines, and acute phase proteins. Orthogonal Partial Least Squares Discriminant Analysis (PLS-DA): the score plot (each dot represents the cytokine profile from each mouse) shows clustering among groups (i.e., peritonitis vs. peritonitis plus benzo-17R-RvD2). The loading plot demonstrates correlations in which the measured cytokines contribute to the cluster separation in the score plot. (C) Relative intensities of cytokines, chemokines, and acute phase proteins. Proteome arrays consist of 40 proteins. Among them 18 were present in the peritoneal exudates. Results are mean ± SEM; relative abundance from n = 4 individual mouse exudates, ∗ p < 0.05, ∗∗ p < 0.01; peritonitis plus benzo-17R-RvD2 versus peritonitis alone; Student’s t test.

    Article Snippet: The supernatant (750 μl) was incubated with the pre-coated Proteome Profiler array membranes (ARY006, R&D Systems) and processed according to the manufacturer’s instructions.

    Techniques: Flow Cytometry