cyclin e1  (Proteintech)

Journal: Cancer research

Article Title: CDK2 Inhibition Exerts RB-Independent Antitumor Activity in CDK4/6 Inhibitor–Resistant HR + /HER2 − Breast Cancer

doi: 10.1158/0008-5472.CAN-25-1859

Figure Lengend Snippet: CDK2 inhibition does not target RB in most of the HR + /HER2 − breast cancers. A and B, Western blot analysis with the indicated antibodies of lysates from the patient-derived HR + MBC cell lines MGH10035.1, MGH358.1, and MGH406.4, and the CCNE1 -amplified TNBC MDA-MB-157 and ovarian cancer OVCAR3 cell lines treated with different concentrations of CDK4/6 (palbociclib), CDK2/4/6 (PF-06873600), or CDK2 (PF-07104091) inhibitors. C, In vitro kinase assay using recombinant cyclin A2/CDK1, cyclin A2/CDK2, and cyclin D3/CDK2 after incubation with increased concentrations of PF-07104091 CDK2i. Data were normalized to incubation with DMSO, and error bars indicate standard deviations ( n = 3). IC 50 values were 1.370 nmol/L for cyclin A2/CDK1, 0.0821 nmol/L for cyclin A2/CDK2, and 3.062 nmol/L for cyclin D3/CDK2. IC 50 (nmol/L) was calculated using nonlinear regression of the dose–response inhibition. D and E, Cell viability of MGH10035.1 ( C ) and MCF7 ( D ) cells expressing shSCR or shRB1 and treated with CDK4/6is, CDK2/4/6is, or CDK2is for 7 days. Data were normalized to DMSO-treated cells. Error bars indicate standard deviations ( n = 3). F, Heatmaps presenting the CDK4/6i, CDK2/4/6i, or CDK2i log IC 50 (nmol/L) in MGH10035.1, MCF7, CAMA1, MDA-MB-361, and MDA-MB-415 HR + breast cancer cell lines. IC 50 was calculated using nonlinear regression of the dose–response inhibition.

Article Snippet: CDK2 (cat. #sc-163, RRID: AB_631215), cyclin D1 (cat. #sc-753, RRID: AB_2070433), and cyclin E1 (cat. #sc-247, RRID: AB_627357) antibodies were purchased from Santa Cruz Biotechnology, and the tubulin (cat. #T9026, RRID: AB_477593) antibody was purchased from Sigma-Aldrich.

Techniques: Inhibition, Western Blot, Analysis, Derivative Assay, Amplification, In Vitro, Kinase Assay, Recombinant, Incubation, Expressing

Journal: Cell Death & Disease

Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma

doi: 10.1038/s41419-026-08593-5

Figure Lengend Snippet: A - C EdU flow plots for Karpas-422, RL, and Sc-1 cells after 24-h treatment (Linperlisib 32 μM; Chiglitazar 16 μM). D – F Quantification of EdU-positive cells showing reduced DNA synthesis with the combination ( n = 3). G – L ( G , H ) for Karpas-422; ( I , J ) for RL; ( K , L ) for Sc-1. Cell-cycle distribution by PI staining, demonstrating G0/G1 accumulation with Linperlisib and further enhancement by the combination (Linperlisib 16 μM; Chiglitazar 8 μM; n = 3). M GSEA indicating negative enrichment of the G1/S transition pathway (NES = − 2.85865, p < 0.00001). N – P Western blots of G1/S checkpoint proteins (p27, Cyclin E1, CDK2, p-CDK2); combination treatment increased p27 and decreased Cyclin E1, CDK2, and p-CDK2 (representative of three independent experiments).

Article Snippet: The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).

Techniques: DNA Synthesis, Staining, Western Blot

Journal: ACS Omega

Article Title: Deciphering the Effect of Methyl 4‑Hydroxybenzoate on Breast Cancer by Bioinformatics and Experiments

doi: 10.1021/acsomega.5c11680

Figure Lengend Snippet: Identification and biomarkers (A–C) PPI network: The interaction of 23 candidate genes (A), CCNE1 (B), and UGT1A6 (C). (D–F) The top 15 genes acquired by Radiality (D), Closeness (E), and Degree algorithms (F). (G) Venn diagram of intersecting genes from the above 3 algorithms with the module genes. (H–I) Box plot analysis indicated the hub genes in the training set and validation set.

Article Snippet: Primary antibodies against Cyclin E1 (HUABIO, ET1612–16; 1:2000), EZH2 (HUABIO, HA722095; 1:2000), E2F1 (HUABIO, ET1701–73; 1:2000), CDK1 (Affinity, DF6024; 1:2000), E-cadherin (Abcam, ab314063; 1:1000), vimentin (Abcam, ab8978; 1:2000), MMP2 (Abcam, ab92536; 1:2000), MMP9 (HUABIO, ET1704–69; 1:4000), and GADPH (HUABIO, HA721136; 1:20,000) were used.

Techniques: Biomarker Discovery

Journal: ACS Omega

Article Title: Deciphering the Effect of Methyl 4‑Hydroxybenzoate on Breast Cancer by Bioinformatics and Experiments

doi: 10.1021/acsomega.5c11680

Figure Lengend Snippet: Pathways enriched with biomarkers in BRCA (A–D) GSEA revealed the enriched pathway of (A) CDK1, (B) E2F1, (C) EZH2, and (D) CCNE1.

Article Snippet: Primary antibodies against Cyclin E1 (HUABIO, ET1612–16; 1:2000), EZH2 (HUABIO, HA722095; 1:2000), E2F1 (HUABIO, ET1701–73; 1:2000), CDK1 (Affinity, DF6024; 1:2000), E-cadherin (Abcam, ab314063; 1:1000), vimentin (Abcam, ab8978; 1:2000), MMP2 (Abcam, ab92536; 1:2000), MMP9 (HUABIO, ET1704–69; 1:4000), and GADPH (HUABIO, HA721136; 1:20,000) were used.

Techniques:

Journal: ACS Omega

Article Title: Deciphering the Effect of Methyl 4‑Hydroxybenzoate on Breast Cancer by Bioinformatics and Experiments

doi: 10.1021/acsomega.5c11680

Figure Lengend Snippet: The binding ability of MEP to biomarkers Molecular docking models of (A) CDK1, (B) E2F1, (C) EZH2, and (D) CCNE1 with MEP. (E) RMSD analysis of protein backbone atoms during 100 ns MD simulations. (F) RMSF analysis of protein backbone atoms during 100 ns MD simulations. (G) Rg analysis of protein backbone atoms during 100 ns simulations.

Article Snippet: Primary antibodies against Cyclin E1 (HUABIO, ET1612–16; 1:2000), EZH2 (HUABIO, HA722095; 1:2000), E2F1 (HUABIO, ET1701–73; 1:2000), CDK1 (Affinity, DF6024; 1:2000), E-cadherin (Abcam, ab314063; 1:1000), vimentin (Abcam, ab8978; 1:2000), MMP2 (Abcam, ab92536; 1:2000), MMP9 (HUABIO, ET1704–69; 1:4000), and GADPH (HUABIO, HA721136; 1:20,000) were used.

Techniques: Binding Assay

Journal: ACS Omega

Article Title: Deciphering the Effect of Methyl 4‑Hydroxybenzoate on Breast Cancer by Bioinformatics and Experiments

doi: 10.1021/acsomega.5c11680

Figure Lengend Snippet: Key cells in the scRNA-seq data (A, B) Relative comparison of spatial clusters between the BRCA and the control samples. (C–F) The violin plot revealed the expression level of (C) CDK1, (D) E2F1, (E) EZH2, and (F) CCNE1 in different cell subsets between the BRCA samples versus. controls. (G) KEGG results for fibroblasts between the BRCA samples versus. controls.

Article Snippet: Primary antibodies against Cyclin E1 (HUABIO, ET1612–16; 1:2000), EZH2 (HUABIO, HA722095; 1:2000), E2F1 (HUABIO, ET1701–73; 1:2000), CDK1 (Affinity, DF6024; 1:2000), E-cadherin (Abcam, ab314063; 1:1000), vimentin (Abcam, ab8978; 1:2000), MMP2 (Abcam, ab92536; 1:2000), MMP9 (HUABIO, ET1704–69; 1:4000), and GADPH (HUABIO, HA721136; 1:20,000) were used.

Techniques: Comparison, Control, Expressing

Journal: ACS Omega

Article Title: Deciphering the Effect of Methyl 4‑Hydroxybenzoate on Breast Cancer by Bioinformatics and Experiments

doi: 10.1021/acsomega.5c11680

Figure Lengend Snippet: MEP promote the malignant biological behavior of MCF-7 (A) qRT-PCR data showing CDK1, EZH2, E2F1, and CCNE1 mRNA expression. (B) Western blots showing CDK1, EZH2, E2F1, and CCNE1 expression. (C) Macrographs of colony formation and quantitative analysis. (D) Flow cytometry showing cell cycle distribution and cell percentages in different phases. (E) Wound healing assay with quantitative analysis was performed to assess the migratory capacity. Scale bar 650 μm. (F) Representative cell migration images and migration index. Scale bar 275 μm. (G) Phalloidin staining was employed to evaluate F-actin reorganization. Scale bar 25 μm. (H) Western blots showing E-cadherin, Vimentin, MMP2, and MMP9 expression. Data are presented as the mean ± standard deviation ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 when compared with control cells.

Article Snippet: Primary antibodies against Cyclin E1 (HUABIO, ET1612–16; 1:2000), EZH2 (HUABIO, HA722095; 1:2000), E2F1 (HUABIO, ET1701–73; 1:2000), CDK1 (Affinity, DF6024; 1:2000), E-cadherin (Abcam, ab314063; 1:1000), vimentin (Abcam, ab8978; 1:2000), MMP2 (Abcam, ab92536; 1:2000), MMP9 (HUABIO, ET1704–69; 1:4000), and GADPH (HUABIO, HA721136; 1:20,000) were used.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Flow Cytometry, Wound Healing Assay, Migration, Staining, Standard Deviation, Control