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cat af791  (R&D Systems)


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    Structured Review

    R&D Systems cat af791
    Cat Af791, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cx3cl1/Mouse+CX3CL1%2FFractalkine+Chemokine+Domain+Antibody/10__3390_slash_ijms27083547-164-10-8
    Average 93 stars, based on 10 article reviews
    cat af791 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Ivaltinostat combination therapy for treating pancreatic cancer
    Article Snippet: .. ELISA Analysis: Serological marker measurement was performed using ELISA kits according to the manufacturer's protocols: IL4 (D4050), TIMP1 (DTM100), IL3 (D3000), ILIA (DLA50), PDGFB (DBB00), TEK (or TIE2, DTE200), PECAM1 (DCD310), MMP2 (MMP200), pro-MMP10 (DM1000), KLK3/PSA (DKK300), free IGFBP1 (DGB100), CX3CL1 (DCX310), IL19 (D1900) (all R&D SYSTEMS); and YAP (LifeSpan BioSciences, Inc., LS-F49700). ..

    Article Title: Alpha-toxin-elicited CX 3 CL1 release in Staphylococcus aureus pneumonia impairs bactericidal function of human monocytes.
    Article Snippet: Whole-slide scanning and photomicrography were performed with a Nano Zoomer 2.0-HT digital slide scanner (Hamamatsu, Houston, TX). .. The levels of CX3CL1 were determined according to the manufacturer’s instructions using the Human CX3CL1/Fractalkine DuoSet ELISA and DuoSet ELISA Ancillary Reagent Kit (R&D Systems, Minneapolis, MN, USA). ..

    Marker:

    Article Title: Ivaltinostat combination therapy for treating pancreatic cancer
    Article Snippet: .. ELISA Analysis: Serological marker measurement was performed using ELISA kits according to the manufacturer's protocols: IL4 (D4050), TIMP1 (DTM100), IL3 (D3000), ILIA (DLA50), PDGFB (DBB00), TEK (or TIE2, DTE200), PECAM1 (DCD310), MMP2 (MMP200), pro-MMP10 (DM1000), KLK3/PSA (DKK300), free IGFBP1 (DGB100), CX3CL1 (DCX310), IL19 (D1900) (all R&D SYSTEMS); and YAP (LifeSpan BioSciences, Inc., LS-F49700). ..

    Incubation:

    Article Title: Neuronal DJ-1 regulates microglial activation in Parkinson’s disease
    Article Snippet: The membrane was blocked with 5% bovine serum albumin in non-fat dry milk (Bio-Rad) to prevent non-specific binding. .. Primary antibodies against the following were incubated with the membrane overnight at 4°C: DJ-1 (rabbit IgG, 1:2000; CST, Danvers, MA, USA; Cat# 5933, RRID: AB_11179085), CX3CL1 (mouse IgG, 1:500; R&D Systems, Minneapolis, MN, USA; Cat# MAB3652, RRID: AB_2087144), glyceraldehyde 3-phosphate dehydrogenase (rabbit IgG, 1:3000; CST; Cat# 2118, RRID: AB_561053), Na-K ATPase (rabbit IgG, 1:1000; CST; Cat# 3010, RRID: AB_2060983), ADAM10 (rabbit IgG, 1:500; R&D Systems; Cat# MAB1427, RRID: AB_2223057), and ADAM17 (rabbit IgG, 1:500; R&D Systems; Cat# AF9301, RRID: AB_10891879). .. Following this incubation, the membrane was washed and incubated with horseradish peroxidase-conjugated secondary antibodies to goat anti-rabbit IgG (1:10,000; Jackson ImmunoResearch, West Grove, PA, USA; Cat# 111-035-144, RRID: AB_2307391) or goat anti-mouse IgG (1:10,000; Jackson ImmunoResearch; Cat# 111-035-003, RRID: AB_2313567).

    Article Title: Upregulation of Cathepsin S Expression Contributes to Neuronal Damage Following Kainic Acid-Induced Status Epilepticus.
    Article Snippet: Coronal brain slices containing the hippocampus were sectioned at 40 μm using a Leica freezing microtome, washed with 0.4% Triton X- 100, and incubated in the blocking solution containing 3% goat serum in PBS. .. After blocking, slices were incubated with primary antibody against Ctss (1:1000; Cat# sc- 74 429, Santa Cruz, Dallas, TX; RRID: AB_1120051), ionized calcium- binding adapter molecule- 1 (Iba1; 1:1000, Cat# ab178847, Abcam, Boston, MA; RRID: AB_2832244), CX3CL1 (1:1000; Cat# MAB571- 100, R&D Systems, Minneapolis, MN), neuron specific nuclear protein (NeuN; 1:1000; Cat# ABN78, Millipore, Billerica, MA; RRID: AB_10807945) or glial fibrillary acidic protein (GFAP; 1:1000, Cat# 13- 0300, Thermo Fisher Scientific, Eugene, OR; RRID: AB_2532994). ..

    Membrane:

    Article Title: Neuronal DJ-1 regulates microglial activation in Parkinson’s disease
    Article Snippet: The membrane was blocked with 5% bovine serum albumin in non-fat dry milk (Bio-Rad) to prevent non-specific binding. .. Primary antibodies against the following were incubated with the membrane overnight at 4°C: DJ-1 (rabbit IgG, 1:2000; CST, Danvers, MA, USA; Cat# 5933, RRID: AB_11179085), CX3CL1 (mouse IgG, 1:500; R&D Systems, Minneapolis, MN, USA; Cat# MAB3652, RRID: AB_2087144), glyceraldehyde 3-phosphate dehydrogenase (rabbit IgG, 1:3000; CST; Cat# 2118, RRID: AB_561053), Na-K ATPase (rabbit IgG, 1:1000; CST; Cat# 3010, RRID: AB_2060983), ADAM10 (rabbit IgG, 1:500; R&D Systems; Cat# MAB1427, RRID: AB_2223057), and ADAM17 (rabbit IgG, 1:500; R&D Systems; Cat# AF9301, RRID: AB_10891879). .. Following this incubation, the membrane was washed and incubated with horseradish peroxidase-conjugated secondary antibodies to goat anti-rabbit IgG (1:10,000; Jackson ImmunoResearch, West Grove, PA, USA; Cat# 111-035-144, RRID: AB_2307391) or goat anti-mouse IgG (1:10,000; Jackson ImmunoResearch; Cat# 111-035-003, RRID: AB_2313567).

    Negative Control:

    Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer’s disease
    Article Snippet: .. The following day, chemoattractant CX3CL1 (20 ng/ml Fractalkine, R&D systems, Minneapolis, MN, USA) was added to the lower chamber while negative control contained 0 ng/ml chemoattractant and positive control contained 20 ng/ml to both upper and lower chamber. ..

    Positive Control:

    Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer’s disease
    Article Snippet: .. The following day, chemoattractant CX3CL1 (20 ng/ml Fractalkine, R&D systems, Minneapolis, MN, USA) was added to the lower chamber while negative control contained 0 ng/ml chemoattractant and positive control contained 20 ng/ml to both upper and lower chamber. ..

    Blocking Assay:

    Article Title: Upregulation of Cathepsin S Expression Contributes to Neuronal Damage Following Kainic Acid-Induced Status Epilepticus.
    Article Snippet: Coronal brain slices containing the hippocampus were sectioned at 40 μm using a Leica freezing microtome, washed with 0.4% Triton X- 100, and incubated in the blocking solution containing 3% goat serum in PBS. .. After blocking, slices were incubated with primary antibody against Ctss (1:1000; Cat# sc- 74 429, Santa Cruz, Dallas, TX; RRID: AB_1120051), ionized calcium- binding adapter molecule- 1 (Iba1; 1:1000, Cat# ab178847, Abcam, Boston, MA; RRID: AB_2832244), CX3CL1 (1:1000; Cat# MAB571- 100, R&D Systems, Minneapolis, MN), neuron specific nuclear protein (NeuN; 1:1000; Cat# ABN78, Millipore, Billerica, MA; RRID: AB_10807945) or glial fibrillary acidic protein (GFAP; 1:1000, Cat# 13- 0300, Thermo Fisher Scientific, Eugene, OR; RRID: AB_2532994). ..

    Binding Assay:

    Article Title: Upregulation of Cathepsin S Expression Contributes to Neuronal Damage Following Kainic Acid-Induced Status Epilepticus.
    Article Snippet: Coronal brain slices containing the hippocampus were sectioned at 40 μm using a Leica freezing microtome, washed with 0.4% Triton X- 100, and incubated in the blocking solution containing 3% goat serum in PBS. .. After blocking, slices were incubated with primary antibody against Ctss (1:1000; Cat# sc- 74 429, Santa Cruz, Dallas, TX; RRID: AB_1120051), ionized calcium- binding adapter molecule- 1 (Iba1; 1:1000, Cat# ab178847, Abcam, Boston, MA; RRID: AB_2832244), CX3CL1 (1:1000; Cat# MAB571- 100, R&D Systems, Minneapolis, MN), neuron specific nuclear protein (NeuN; 1:1000; Cat# ABN78, Millipore, Billerica, MA; RRID: AB_10807945) or glial fibrillary acidic protein (GFAP; 1:1000, Cat# 13- 0300, Thermo Fisher Scientific, Eugene, OR; RRID: AB_2532994). ..

    Staining:

    Article Title: Tumor cell heterogeneity drives spatial organization of the intratumoral immune response.
    Article Snippet: Intratumoral heterogeneity (ITH)—defined as genetic and cellular diversity within a tumor—is linked to failure of immunotherapy and an inferior anti-tumor immune response.. We modeled heterogeneous tumors comprised of “hot” and “cold” tumor populations (giving rise to T cell–rich and T cell–poor tumors, respectively) and introduced fluorescent labels to enable precise spatial tracking.. We found the cold tumor cell population exerted a “dominant cold” effect in mixed tumors.

    Bioprocessing:

    Article Title: Expression and analysis of CX3CL1 chemokine and CD57+ lymphocytes in oral squamous cell carcinoma and their correlation with clinicopathologic features.
    Article Snippet: .. Sections were subjected to mouse monoclonal antibodies for CX3CL1 Clone ‐MAB3651 (R & D Systems, dilution 1:30 USA) for 1.5 h and CD57 Clone‐TB01 (Dako, Denmark) for 25 min. .. Secondary antibodies of Dako and Master Diagnostics were used for CX3CL1 and CD57, respectively, for 30 min, following rinsing with tris buffered saline.



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