Journal: mSphere
Article Title: LINC2781 enhances antiviral immunity against coxsackievirus B5 infection by activating the JAK-STAT pathway and blocking G3BP2-mediated STAT1 degradation
doi: 10.1128/msphere.00062-25
Figure Lengend Snippet: The characterization of LINC2781. (A) A schematic representation of the human LINC2781 transcribed from the XLOC_262866 gene; (B) SH-SY5Y and THP-1 cells were infected with CVB5 at increasing MOIs for 24 h or CVB5 (MOI = 1) for the indicated time intervals. The expression of LINC2781 was measured by RT-qPCR; (C) SH-SY5Y cells were transfected with increasing amounts of CVB5 RNA or plasmids (2 µg) encoding viral nonstructural proteins. The expression of LINC2781 was measured by RT-qPCR; (D) SH-SY5Y cells were infected with CVB5, CVA16, EV71, HSV-1, and RABV (MOI = 1) for 24 h. The expression of LINC2781 was measured by RT-qPCR; (E) SH-SY5Y cells were stimulated with different amounts of poly (I:C) or poly (dG:dC) for 24 h. The expression of LINC2781 was measured by RT-qPCR; (F) Various cell lines were infected with CVB5 (MOI = 1) for 24 h. The expression of LINC2781 was measured by RT-qPCR; (G) Three-day-old BALB/c mice were infected with CVB5 (20 LD50) for 10 days. The expression of LINC2781 was measured by RT-qPCR. (H) SH-SY5Y cells were treated with increasing amounts of IFN-β, IFN-γ, LPS, and TNF-α for 24 h. The expression of LINC2781 was measured by RT-qPCR. Biologically independent experiments ( n = 3) were conducted, and all data were shown as mean ± SD. Student’s t -test was used to detect significant differences, with P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and ns for no significant difference.
Article Snippet: Coxsackievirus A16 (CVA16) strain 74/YN/2016 (GenBank: KY440934.1 ), enterovirus A71 (EV71) strain RA330/YN/CHN/2009 (GenBank: MK028135.1 ), human herpes virus 1 (HSV-1) isolate ZW6 (GenBank: KX424525.1 KX424525.1 ), and rabies virus CVS-11 (RABV, ATCC VR 959) were stored in our laboratory.
Techniques: Infection, Expressing, Quantitative RT-PCR, Transfection