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Schematic representation of fabrication of peptide-functionalized PEG hydrogel microarrays. A. The printing solutions composed of PEGDA monomer and various methacrylated peptides were prepared in a 384-well plate. B. The printing solutions were placed onto poly(HEMA) coated microscope slides with a customized <t>microarrayer</t> and polymerized by UV under Argon protection to prepare peptide-functionalized PEG hydrogel spots. Eight hydrogel spots in a microarray were shown to present the dimension of the hydrogel spots and the distance between the hydrogel spots. C. High throughput analysis of cellular activities after cell seeding onto the microarray.
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Schematic representation of fabrication of peptide-functionalized PEG hydrogel microarrays. A. The printing solutions composed of PEGDA monomer and various methacrylated peptides were prepared in a 384-well plate. B. The printing solutions were placed onto poly(HEMA) coated microscope slides with a customized microarrayer and polymerized by UV under Argon protection to prepare peptide-functionalized PEG hydrogel spots. Eight hydrogel spots in a microarray were shown to present the dimension of the hydrogel spots and the distance between the hydrogel spots. C. High throughput analysis of cellular activities after cell seeding onto the microarray.

Journal: Acta biomaterialia

Article Title: Development of peptide-functionalized synthetic hydrogel microarrays for stem cell and tissue engineering applications

doi: 10.1016/j.actbio.2016.09.006

Figure Lengend Snippet: Schematic representation of fabrication of peptide-functionalized PEG hydrogel microarrays. A. The printing solutions composed of PEGDA monomer and various methacrylated peptides were prepared in a 384-well plate. B. The printing solutions were placed onto poly(HEMA) coated microscope slides with a customized microarrayer and polymerized by UV under Argon protection to prepare peptide-functionalized PEG hydrogel spots. Eight hydrogel spots in a microarray were shown to present the dimension of the hydrogel spots and the distance between the hydrogel spots. C. High throughput analysis of cellular activities after cell seeding onto the microarray.

Article Snippet: The microarrays were printed in a humid Ar-atmosphere on epoxy monolayer-coated glass slides (Xenopore XENOSLIDE E, Hawthorne, NJ) that were first dip-coated in 4 v/v% poly(hydroxyethyl methacrylate) (i.e., poly(HEMA)) using a customized microarrayer (Biodot).

Techniques: Microscopy, Microarray, High Throughput Screening Assay