Journal: Food Science & Nutrition
Article Title: Sulforaphene Mitigates Periodontitis by NRF2‐Dependent Regulation of P. gingivalis ‐Induced Inflammatory Response and Bone Homeostasis
doi: 10.1002/fsn3.71319
Figure Lengend Snippet: LFS impedes osteoclast differentiation of BMDM in vitro. (A, B) Representative images of TRAP staining of RANKL‐induced BMDM with different concentration of LFS (0, 1, and 5 μM) and quantitative analysis. Scale bar = 100 μm. (C, D) Representative images of F‐actin ring staining (phalloidin, red) and nuclei (DAPI, blue) of BMDMs, and subsequent quantitative analysis of osteoclast area and the number of nuclei per osteoclast. Scale bar = 50 μm. (E–J) Relative mRNA expressions of osteoclast differentiation related genes of BMDM with LFS (0, 1, and 5 μM) by qPCR. (K–O) Representative western blot images of NFATC1, MMP9, CTSK, ATP6V0D2 and β‐TUBULIN of BMDM with LFS and respective quantitative analysis. Data are presented as means ± SD of 3 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
Article Snippet: Following blocked with blocking buffer (Beyotime, China), the membranes were incubated overnight at 4°C with primary antibodies specific to SP7 (Catalogue number: ab209484, Abcam, USA), OCN (Catalogue number: b0822, Santa Cruz Biotechnology, USA), NFATC1 (Catalogue number: d0522, Santa Cruz Biotechnology, USA), IL‐1β (Catalogue number: A19635, ABclonal Technology, China), IL‐6 (Catalogue number: A0286, ABclonal Technology, China), CTSK (Catalogue number: PB9856, Bosterbio, China), MMP9 (Catalogue number: ab76003, Abcam, USA), ATP6V0D2 (Catalogue number: 333641‐ap, Proteintech, China), TRAP (Catalogue number: ab133238, Abcam, USA), and β‐TUBULIN (Catalogue number: M20045F, Abmart, China) at 4°C overnight.
Techniques: In Vitro, Staining, Concentration Assay, Western Blot