Journal: Cellular & Molecular Biology Letters

Article Title: KIAA1429-mediated M 6 A methylation inhibits osteoclast differentiation via stabilizing Lrp4 mRNA and protects against osteoporosis

doi: 10.1186/s11658-025-00800-z

Figure Lengend Snippet: Kiaa1429 overexpression could not rescue bone loss by LRP4 deficiency in vivo. A Flowchart of the AAV injection experiment into the bone marrow cavity in Ctsk -CreERT2; Lrp4 fl/fl OVX mice and Lrp4 fl/fl OVX mice. B Representative micro-CT images of trabecular bone from the femoral metaphysis in Ctsk -CreERT2; Lrp4 fl/fl OVX mice and those of other groups. C Micro-CT measurements of Tb. BV/TV; Tb. Th; Tb. No.; Tb. Sp; and BMD in the femurs of Ctsk -CreERT2; Lrp4 fl/fl OVX mice after treatment with AAV-NC or AAV-KIAA1429. D Representative images of H&E staining and quantitative analysis in Ctsk -CreERT2; Lrp4 fl/fl OVX mice and Lrp4 fl/fl OVX mice after treatment with AAV-NC or AAV-KIAA1429. E Representative images of Masson staining and quantitative analysis in Ctsk -CreERT2; Lrp4 fl/fl OVX mice and Lrp4 fl/fl OVX mice after treatment with AAV-NC or AAV-KIAA1429. F Representative images of TRAP staining and quantitative analysis in Ctsk -CreERT2; Lrp4 fl/fl OVX mice and Lrp4 fl/fl OVX mice after treatment with AAV-NC or AAV-KIAA1429. G Representative images of immunohistochemistry staining for CTSK and quantitative analysis in Ctsk -CreERT2; Lrp4 fl/fl OVX mice, and Lrp4 fl/fl OVX mice after treatment with AAV-NC or AAV-KIAA1429. The values are mean ± SD of at least three independent experiments; n.s. p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The Ctsk -CreERT2 transgenic line was acquired from Cyagen (USA).

Techniques: Over Expression, In Vivo, Injection, Micro-CT, Staining, Immunohistochemistry

Journal: Cellular & Molecular Biology Letters

Article Title: KIAA1429-mediated M 6 A methylation inhibits osteoclast differentiation via stabilizing Lrp4 mRNA and protects against osteoporosis

doi: 10.1186/s11658-025-00800-z

Figure Lengend Snippet: KIAA1429-mediated LRP4-regulated TNFAIP3 in osteoclasts. A Kyoto Encyclopedia of Genes and Genome (KEGG) enrichment analysis of signaling pathway based on RNA-seq and MeRIP-seq. B qRT-PCR was used to determine the alterations in mRNA expression of DEGs upon Kiaa1429 overexpression. C Western blotting was performed for TNFAIP3 in Kiaa1429 -overexpressed RAW264.7 cells. D , E qRT-PCR and western blotting were performed for TNFAIP3 in Lrp4 -overexpressed cells. F Representative images of immunohistochemistry staining for TNFAIP3 and quantitative analysis in Ctsk -CreERT2; Lrp4 fl/fl and Lrp4 fl/fl OVX mice. G , H TNFAIP3 was measured by qRT-PCR and western blotting in RAW264.7 and BMDM during osteoclast differentiation. I , J The effect of Lrp4 knockout on TNFAIP3 expression was measured by qRT-PCR and western blotting after Kiaa1429 overexpression. K , L , M The effect of Lrp4 overexpression on TNFAIP3 expression was measured by qRT-PCR and western blotting after Kiaa1429 knockout. N Co-immunoprecipitation of TNFAIP3 with endogenous LRP4 in RAW264.7 and BMDM. O Co-localization of LRP4 and TNFAIP3 after treatment with or without RANKL in RAW264.7 cells was exhibited in confocal immunofluorescent images. The values are mean ± SD of at least three independent experiments; n.s. p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The Ctsk -CreERT2 transgenic line was acquired from Cyagen (USA).

Techniques: RNA Sequencing, Quantitative RT-PCR, Expressing, Over Expression, Western Blot, Immunohistochemistry, Staining, Knock-Out, Immunoprecipitation

Journal: Experimental Biology and Medicine

Article Title: Impaired fracture healing is associated with callus chondro-osseous junction abnormalities in periostin-null and osteopontin-null mice

doi: 10.3389/ebm.2024.10066

Figure Lengend Snippet: Study rabbit antibodies.

Article Snippet: CTSK (cathepsin K) , Novus Biologicals , NBP1-45460 , PAb , 1:100 , 10 mM sodium citrate, 1 h, 75°C.

Techniques:

Journal: Journal of Bone and Mineral Metabolism

Article Title: Non-contact electrical stimulation via a Vector-potential transformer promotes bone healing in drill-hole injury model

doi: 10.1007/s00774-025-01603-0

Figure Lengend Snippet: Bone resorption in the drill-hole injury. a Representative images of TRAP staining on day 21 after bone injury. Arrowheads: TRAP-positive cells. *: Bone matrix. Dotted line: Bone surface. Scale bar = 100 µm. TRAP, tartrate-resistant acid phosphatase. b Quantification of TRAP-positive cells. n = 3. c Relative mRNA expression of Ctsk . n = 3−6 per time point. ‡p < 0.05 (between CO and BI). †p < 0.05 (between CO and VP). *p < 0.05 (between BI and VP). CO Control, BI Bone injury, VP Vector-potential

Article Snippet: TaqMan probes were used for Gapdh (Rn01775763_g1, Invitrogen) as the housekeeping gene, and Bglap (encoding osteocalcin, Rn00566386_g1, Invitrogen) and Ctsk (encoding cathepsin K, Rn00580723_m1, Invitrogen) as target genes.

Techniques: Staining, Expressing, Control, Plasmid Preparation