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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis
doi: 10.3389/fcell.2021.820274
Figure Lengend Snippet: Validation of UMAP data with immunochemistry analysis of nonimmune cells in CAP (antibody-positive). Immunochemistry staining showed the location of CTSK for osteo-like (osteoclast) cells (A) , BCAM for basal/stromal cells (B) , VWF for endothelial cells (C) , and ODAM for odontogenic epithelial cells (D) in a subset of CAP lesions (left micrograph). Images were taken at ×400 magnification. Scale bars, 50 μm. The relevant transcriptome data visualized in a UMAP plot from the three samples (right pictures).
Article Snippet: Next, the sections were incubated overnight with primary antibodies against CD3 (1:200 Proteintech; #17617-1-AP), CD19 (1:200 Bioss; #bs-0079), CD14 (1:50 Proteintech; #17000-1-AP), CD117 (1:200
Techniques: Staining
Journal: Journal of Bone and Mineral Metabolism
Article Title: Non-contact electrical stimulation via a Vector-potential transformer promotes bone healing in drill-hole injury model
doi: 10.1007/s00774-025-01603-0
Figure Lengend Snippet: Bone resorption in the drill-hole injury. a Representative images of TRAP staining on day 21 after bone injury. Arrowheads: TRAP-positive cells. *: Bone matrix. Dotted line: Bone surface. Scale bar = 100 µm. TRAP, tartrate-resistant acid phosphatase. b Quantification of TRAP-positive cells. n = 3. c Relative mRNA expression of Ctsk . n = 3−6 per time point. ‡p < 0.05 (between CO and BI). †p < 0.05 (between CO and VP). *p < 0.05 (between BI and VP). CO Control, BI Bone injury, VP Vector-potential
Article Snippet: TaqMan probes were used for Gapdh (Rn01775763_g1, Invitrogen) as the housekeeping gene, and Bglap (encoding osteocalcin, Rn00566386_g1, Invitrogen) and Ctsk (encoding cathepsin K,
Techniques: Staining, Expressing, Control, Plasmid Preparation