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crl 2992  (ATCC)


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    ATCC crl 2992
    Crl 2992, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crl+2992/Mfn1-null+MEFs/bio_rxiv__64898__2026__05__07__723496-49-6-12
    Average 93 stars, based on 52 article reviews
    crl 2992 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Atypical peripheral actin band formation via overactivation of RhoA and nonmuscle myosin II in mitofusin 2-deficient cells
    Article Snippet: Cell line ( M. musculus ) , Mfn1 -null MEF , ATCC , CRL-2992 , .

    Article Title: TFEB and MCOLN1 are important for Coxiella burnetii egress via lysosomal exocytosis
    Article Snippet: EA.hy926 (human umbilical vein endothelial cells), CRL-2992, LOT 63396642 were purchased from ATCC.

    Article Title: Burst mitofusin activation reverses neuromuscular dysfunction in murine CMT2A
    Article Snippet: Mfn1 null M. musculus , Mfn1 null MEFs , ATCC , CRL-2992 , Murine embryonic fibroblasts.



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    a . Time-lapse sequence (in minutes) illustrating the clustering of P5CS-GFP in MEFs through mitochondrial fusion. P5CS-GFP clustering was monitored 7 hours after switching cells to galactose media, capturing two distinct events (white arrows and orange arrows). See also Supplementary Video 2 . b , c . Western blot analysis of MEFs defective in mitochondrial fusion. HA-tagged MFN2 ( b ) or Flag-tagged <t>MFN1</t> ( c ) cDNA was introduced to restore the ability of cells to engage in mitochondrial fusion. Actin is used as a loading control. d . Representative immunofluorescence images of endogenous P5CS and ATP5B in Mfn1 −/− MEFs expressing either empty vector (Empty) or Flag-tagged MFN1 (MFN1-Flag). MEFs were cultured in galactose medium for 8 hours. e . Western blot of DRP1 expression in wildtype, Drp1 −/− , and Drp1 −/− MEF rescued with DRP1 cDNA. Actin is used as a loading control. f . Representative immunostained images of endogenous P5CS, ATP5B, and TOM20 from Mff , Fis1 , Mid49 , Mid51 quadruple knockout (QKO) MEFs cultured in galactose medium for 8 hours. Relative pixel intensity plots corresponding to orange lines in gated area are shown on the right panels of each figure. g . Steady-state proline level measured by GC-MS in wildtype or QKO MEFs. Values are shown relative to the mean of WT MEFs. For all immunofluorescence images, DAPI was used to stain the nucleus. Scale bars are indicated as follows: 5 μm for panels a , d , and f , and 2 μm for all insets and enlarged images within panels a , d , and f . Data are presented as mean ± s.d. from n = 3 independent replicates. Statistical significance was determined using two-tailed t-tests.
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    Image Search Results


    a . Time-lapse sequence (in minutes) illustrating the clustering of P5CS-GFP in MEFs through mitochondrial fusion. P5CS-GFP clustering was monitored 7 hours after switching cells to galactose media, capturing two distinct events (white arrows and orange arrows). See also Supplementary Video 2 . b , c . Western blot analysis of MEFs defective in mitochondrial fusion. HA-tagged MFN2 ( b ) or Flag-tagged MFN1 ( c ) cDNA was introduced to restore the ability of cells to engage in mitochondrial fusion. Actin is used as a loading control. d . Representative immunofluorescence images of endogenous P5CS and ATP5B in Mfn1 −/− MEFs expressing either empty vector (Empty) or Flag-tagged MFN1 (MFN1-Flag). MEFs were cultured in galactose medium for 8 hours. e . Western blot of DRP1 expression in wildtype, Drp1 −/− , and Drp1 −/− MEF rescued with DRP1 cDNA. Actin is used as a loading control. f . Representative immunostained images of endogenous P5CS, ATP5B, and TOM20 from Mff , Fis1 , Mid49 , Mid51 quadruple knockout (QKO) MEFs cultured in galactose medium for 8 hours. Relative pixel intensity plots corresponding to orange lines in gated area are shown on the right panels of each figure. g . Steady-state proline level measured by GC-MS in wildtype or QKO MEFs. Values are shown relative to the mean of WT MEFs. For all immunofluorescence images, DAPI was used to stain the nucleus. Scale bars are indicated as follows: 5 μm for panels a , d , and f , and 2 μm for all insets and enlarged images within panels a , d , and f . Data are presented as mean ± s.d. from n = 3 independent replicates. Statistical significance was determined using two-tailed t-tests.

    Journal: Nature

    Article Title: Cellular ATP demand creates metabolically distinct subpopulations of mitochondria

    doi: 10.1038/s41586-024-08146-w

    Figure Lengend Snippet: a . Time-lapse sequence (in minutes) illustrating the clustering of P5CS-GFP in MEFs through mitochondrial fusion. P5CS-GFP clustering was monitored 7 hours after switching cells to galactose media, capturing two distinct events (white arrows and orange arrows). See also Supplementary Video 2 . b , c . Western blot analysis of MEFs defective in mitochondrial fusion. HA-tagged MFN2 ( b ) or Flag-tagged MFN1 ( c ) cDNA was introduced to restore the ability of cells to engage in mitochondrial fusion. Actin is used as a loading control. d . Representative immunofluorescence images of endogenous P5CS and ATP5B in Mfn1 −/− MEFs expressing either empty vector (Empty) or Flag-tagged MFN1 (MFN1-Flag). MEFs were cultured in galactose medium for 8 hours. e . Western blot of DRP1 expression in wildtype, Drp1 −/− , and Drp1 −/− MEF rescued with DRP1 cDNA. Actin is used as a loading control. f . Representative immunostained images of endogenous P5CS, ATP5B, and TOM20 from Mff , Fis1 , Mid49 , Mid51 quadruple knockout (QKO) MEFs cultured in galactose medium for 8 hours. Relative pixel intensity plots corresponding to orange lines in gated area are shown on the right panels of each figure. g . Steady-state proline level measured by GC-MS in wildtype or QKO MEFs. Values are shown relative to the mean of WT MEFs. For all immunofluorescence images, DAPI was used to stain the nucleus. Scale bars are indicated as follows: 5 μm for panels a , d , and f , and 2 μm for all insets and enlarged images within panels a , d , and f . Data are presented as mean ± s.d. from n = 3 independent replicates. Statistical significance was determined using two-tailed t-tests.

    Article Snippet: Mfn1 −/− (CRL-2992), Opa1 −/− (CRL-2995), Mfn1 −/− and Mfn2 −/− double knockout MEF (CRL-2994) and corresponding wildtype MEFs (CRL-2991) were obtained from the ATCC.

    Techniques: Sequencing, Western Blot, Control, Immunofluorescence, Expressing, Plasmid Preparation, Cell Culture, Quadruple Knockout, Gas Chromatography-Mass Spectrometry, Staining, Two Tailed Test