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crispri vector  (TaKaRa)


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    TaKaRa crispri vector
    Crispri Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispri+vector/Puromycin/bio_rxiv__2025__10__21__683665-133-1-10
    Average 96 stars, based on 3506 article reviews
    crispri vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Benchmarking Perturbation Tools for the Noncoding Genome
    Article Snippet: For CRISPR KO, the lentiCRISPRv2 (Addgene, #52961) vector was used. .. For CRISPRi vector (plenti-dCas9m4-KRAB-MECP2-puro), the plasmid was assembled with XbaI (Takara, #1634) and BamHI (Takara, #1605) digested lentiCRISPRv2 vector, the Cas9-encoding sequence with four amino acid mutations (D10A, D839A, H840A, and N863A), and the KRAB-MeCP2 fragment amplified from dCas9-KRAB-MeCP2 vector (Addgene, #110821) via Gibson assembly. .. For dual-SpCas9, the pLenti-Cas9-Blast vector (Addgene, #52962) was used to generate the Cas9-expressing cell line, and the plentiGuide-Puro vector (Addgene, #52963) served as the pgRNA expression vector.

    Sequencing:

    Article Title: Benchmarking Perturbation Tools for the Noncoding Genome
    Article Snippet: For CRISPR KO, the lentiCRISPRv2 (Addgene, #52961) vector was used. .. For CRISPRi vector (plenti-dCas9m4-KRAB-MECP2-puro), the plasmid was assembled with XbaI (Takara, #1634) and BamHI (Takara, #1605) digested lentiCRISPRv2 vector, the Cas9-encoding sequence with four amino acid mutations (D10A, D839A, H840A, and N863A), and the KRAB-MeCP2 fragment amplified from dCas9-KRAB-MeCP2 vector (Addgene, #110821) via Gibson assembly. .. For dual-SpCas9, the pLenti-Cas9-Blast vector (Addgene, #52962) was used to generate the Cas9-expressing cell line, and the plentiGuide-Puro vector (Addgene, #52963) served as the pgRNA expression vector.

    Amplification:

    Article Title: Benchmarking Perturbation Tools for the Noncoding Genome
    Article Snippet: For CRISPR KO, the lentiCRISPRv2 (Addgene, #52961) vector was used. .. For CRISPRi vector (plenti-dCas9m4-KRAB-MECP2-puro), the plasmid was assembled with XbaI (Takara, #1634) and BamHI (Takara, #1605) digested lentiCRISPRv2 vector, the Cas9-encoding sequence with four amino acid mutations (D10A, D839A, H840A, and N863A), and the KRAB-MeCP2 fragment amplified from dCas9-KRAB-MeCP2 vector (Addgene, #110821) via Gibson assembly. .. For dual-SpCas9, the pLenti-Cas9-Blast vector (Addgene, #52962) was used to generate the Cas9-expressing cell line, and the plentiGuide-Puro vector (Addgene, #52963) served as the pgRNA expression vector.



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    Image Search Results


    A Left: Evolutionary tree of selected mammalian species. Right: Percent base conservation of human LPS-inducible macrophage lncRNA sequences across the genomes of indicated species, sorted by evolutionary distance; top panel includes all 24 lncRNAs from Fig. and data are shown as box plots (median and 75th-25th percentile interquartile range, whiskers indicate minimum and maximum data values), with species generation time overlayed; bottom panel focuses on specific lncRNAs. B Illustration of genomic locations of specified lncRNAs relative to nearest neighboring genes (distance in kilobases [kb] provided). Red triangles indicate transcriptional start site positions targeted by CRISPRi for lncRNA silencing. C Volcano plots from CRISPRi-based lncRNA loss-of-function experiments in THP1 cells stimulated with LPS for 8 h. Fold-changes (fc) compare lncRNA-knockdown cells to empty vector control cells. Results from three independent experiments. Two-tailed Student’s t-test p -values are shown. D Cytoscape network of lncRNAs (blue) and mRNAs from panel C , regulated upon lncRNA silencing (≥2-fold up or down, p ≤ 0.05, two-tailed Student’s t-test). E Pie charts showing proportions of all expressed mRNAs or LPS-responsive mRNAs (≥2-fold up or down, p ≤ 0.05, two-tailed Student’s t-test) affected by silencing of one or more lncRNAs. C – E: 8 h LPS-stimulated THP1 cells and three independent replicates.

    Journal: Nature Communications

    Article Title: A searchable atlas of pathogen-sensitive lncRNA networks in human macrophages

    doi: 10.1038/s41467-025-60084-x

    Figure Lengend Snippet: A Left: Evolutionary tree of selected mammalian species. Right: Percent base conservation of human LPS-inducible macrophage lncRNA sequences across the genomes of indicated species, sorted by evolutionary distance; top panel includes all 24 lncRNAs from Fig. and data are shown as box plots (median and 75th-25th percentile interquartile range, whiskers indicate minimum and maximum data values), with species generation time overlayed; bottom panel focuses on specific lncRNAs. B Illustration of genomic locations of specified lncRNAs relative to nearest neighboring genes (distance in kilobases [kb] provided). Red triangles indicate transcriptional start site positions targeted by CRISPRi for lncRNA silencing. C Volcano plots from CRISPRi-based lncRNA loss-of-function experiments in THP1 cells stimulated with LPS for 8 h. Fold-changes (fc) compare lncRNA-knockdown cells to empty vector control cells. Results from three independent experiments. Two-tailed Student’s t-test p -values are shown. D Cytoscape network of lncRNAs (blue) and mRNAs from panel C , regulated upon lncRNA silencing (≥2-fold up or down, p ≤ 0.05, two-tailed Student’s t-test). E Pie charts showing proportions of all expressed mRNAs or LPS-responsive mRNAs (≥2-fold up or down, p ≤ 0.05, two-tailed Student’s t-test) affected by silencing of one or more lncRNAs. C – E: 8 h LPS-stimulated THP1 cells and three independent replicates.

    Article Snippet: Stable knockdown cell lines were generated using the CRISPR interference (CRISPRi) vector pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2a-GFP (Addgene #71237).

    Techniques: Knockdown, Plasmid Preparation, Control, Two Tailed Test