crispr guide rna design tool (Benchling Inc)
Structured Review
Crispr Guide Rna Design Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispr+tool/crispr+design+tool/pmc13155868-62-34-40
Average 86 stars, based on 1 article reviews
Images
Related Articles
CRISPR:Article Title: Exploring the functions of JAKMIP1 in neuronal IL-6/STAT3 signaling and its relevance to chromosome 15q-duplication syndrome Article Snippet: Microscope slides were imaged either on the upright DM4B LED ( Leica Microsystems ) or inverted EVOS FLoidTM ( Invitrogen TM), DMi8 widefield ( Leica Microsystems ) and TCS SP8 confocal microscopes ( Leica Microsystems ). .. A CRISPR guide RNA (gRNA) sequence was designed to exon 5 of transcript ENST00000282924.9 of the JAKMIP1 gene using the Article Title: Sleeping ORANGE: A CRISPR-Transposase Hybrid Approach to Boost Endogenous Protein Tagging Efficiency Article Snippet: .. A gRNA sequences for CYFIP1 (5’-CGCGTCCTCCAGAGTCACCT-3’), JAKMIP1 (5’-GTCGAAGAAAGGCCGGAGCA-3’) and STAT3 (5’-GCTGCTGTAGCTGATTCCAT-3’) were designed for using the Article Title: Rsc1 DNA-binding bromodomain drives RSC activity at A-rich promoters Article Snippet: Sequences encoding these tags were added to RSC1 and RSC2 loci by two successive rounds of marker-less CRISPR/Cas9 genome editing, using pWS158 (Addgene 90517) and pWS171 (Addgene 90518) as Cas9 expression vectors, as previously described ( ). .. For each RSC1 and RSC2 , a protospacer adjacent motifs (PAM) sequence within 30 nucleotides of the start codon was selected and a guide RNA (gRNA) was designed using the Article Title: Disrupting the LINC complex for treating laminopathy Article Snippet: .. Human Guide RNA Sequences Potential guide RNA sequences to disrupt human SYNE1 KASH domain or SUN1 SUN domain were determined using Article Title: MLL2 facilitates long-range gene regulation through LINE1 elements Article Snippet: For the multilineage differentiation, cells were grown on 0.1% gelatin-coated plates, using KnockOut TM DMEM supplemented with 10% FBS, 2 mM L-glutamine (Thermo Fisher Scientific, 25030024), 0.1 nM β-mercaptoethanol, 1x NEAA, 1x antibiotic and antimycotic solution and 1 μM retinoic acid (RA; Sigma-Aldrich, R2625) for 4 days, as previously described by . .. For the generation of MLL1 -KO, Cxcl12 L1-KO, Cnrip1 L1-KO and Tcstv3 L1-KO cell lines, two sg-RNAs were designed flanking the region to-be deleted for each line, using Benchling’s Article Title: 10 years of CRISPR/CAS genomic engineering in Yarrowia lipolytica. Article Snippet: .. Benchling provides a Article Title: CRISPR-Cas9-based electrochemical biosensor for the detection of katG gene mutations in isoniazid-resistant tuberculosis Article Snippet: .. A mutation-specific gRNA was designed using the Sequencing:Article Title: Exploring the functions of JAKMIP1 in neuronal IL-6/STAT3 signaling and its relevance to chromosome 15q-duplication syndrome Article Snippet: Microscope slides were imaged either on the upright DM4B LED ( Leica Microsystems ) or inverted EVOS FLoidTM ( Invitrogen TM), DMi8 widefield ( Leica Microsystems ) and TCS SP8 confocal microscopes ( Leica Microsystems ). .. A CRISPR guide RNA (gRNA) sequence was designed to exon 5 of transcript ENST00000282924.9 of the JAKMIP1 gene using the Article Title: Sleeping ORANGE: A CRISPR-Transposase Hybrid Approach to Boost Endogenous Protein Tagging Efficiency Article Snippet: .. A gRNA sequences for CYFIP1 (5’-CGCGTCCTCCAGAGTCACCT-3’), JAKMIP1 (5’-GTCGAAGAAAGGCCGGAGCA-3’) and STAT3 (5’-GCTGCTGTAGCTGATTCCAT-3’) were designed for using the Article Title: Rsc1 DNA-binding bromodomain drives RSC activity at A-rich promoters Article Snippet: Sequences encoding these tags were added to RSC1 and RSC2 loci by two successive rounds of marker-less CRISPR/Cas9 genome editing, using pWS158 (Addgene 90517) and pWS171 (Addgene 90518) as Cas9 expression vectors, as previously described ( ). .. For each RSC1 and RSC2 , a protospacer adjacent motifs (PAM) sequence within 30 nucleotides of the start codon was selected and a guide RNA (gRNA) was designed using the Software:Article Title: Sleeping ORANGE: A CRISPR-Transposase Hybrid Approach to Boost Endogenous Protein Tagging Efficiency Article Snippet: .. A gRNA sequences for CYFIP1 (5’-CGCGTCCTCCAGAGTCACCT-3’), JAKMIP1 (5’-GTCGAAGAAAGGCCGGAGCA-3’) and STAT3 (5’-GCTGCTGTAGCTGATTCCAT-3’) were designed for using the Cloning:Article Title: Sleeping ORANGE: A CRISPR-Transposase Hybrid Approach to Boost Endogenous Protein Tagging Efficiency Article Snippet: .. A gRNA sequences for CYFIP1 (5’-CGCGTCCTCCAGAGTCACCT-3’), JAKMIP1 (5’-GTCGAAGAAAGGCCGGAGCA-3’) and STAT3 (5’-GCTGCTGTAGCTGATTCCAT-3’) were designed for using the Clone Assay:Article Title: Rsc1 DNA-binding bromodomain drives RSC activity at A-rich promoters Article Snippet: Sequences encoding these tags were added to RSC1 and RSC2 loci by two successive rounds of marker-less CRISPR/Cas9 genome editing, using pWS158 (Addgene 90517) and pWS171 (Addgene 90518) as Cas9 expression vectors, as previously described ( ). .. For each RSC1 and RSC2 , a protospacer adjacent motifs (PAM) sequence within 30 nucleotides of the start codon was selected and a guide RNA (gRNA) was designed using the other:Article Title: Solid phase transitions as a solution to the genome folding paradox. Article Snippet: Ultra-long-range genomic contacts, which are key components of neuronal genome architecture, constitute a biochemical enigma.. This is because regulatory DNA elements make selective and stable contacts with DNA sequences located hundreds of kilobases away, instead of interacting with proximal sequences occupied by the exact same transcription factors.. This is exemplified in olfactory sensory neurons (OSNs), in which only a fraction of LHX2-, EBF1and LDB1-bound sites interact with each other, converging into highly selective multi-chromosomal enhancer hubs. Mutagenesis:Article Title: CRISPR-Cas9-based electrochemical biosensor for the detection of katG gene mutations in isoniazid-resistant tuberculosis Article Snippet: .. A mutation-specific gRNA was designed using the |
