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Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and <t>TLR9</t> in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.
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Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and TLR9 in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.

Journal: Nephrology Dialysis Transplantation

Article Title: Plasmacytoid dendritic cells modulate the production of mucosal IgA in IgA nephropathy

doi: 10.1093/ndt/gfaf216

Figure Lengend Snippet: Frequency of tonsillar pDCs positively correlates with the expression of mRNAs encoding APRIL, BAFF and TLR9 in tonsillar MNCs from patients with IgAN. Correlation analysis was performed between the frequency of tonsillar pDCs and gene expression in tonsillar MNCs from patients with IgAN ( n = 10, red dots) and CT ( n = 9, black dots). ( A ) Correlation between the percentage of tonsillar pDCs and the expression of TLR9 in tonsillar MNCs from patients with IgAN and CT. ( B ) Correlation between the percentage of tonsillar pDCs and the expression of IL-6 in tonsillar MNCs from patients with IgAN and CT. ( C ) Correlation between the percentage of tonsillar pDCs and the expression of BAFF in tonsillar MNCs from patients with IgAN and CT. ( D ) Correlation between the percentage of tonsillar pDCs and the expression of APRIL in tonsillar MNCs from patients with IgAN and CT. Spearman’s correlation coefficients (r) and P -values are shown in each panel. * P ≤ .05.

Article Snippet: The TLR9 agonist class C CpG-ODN (ODN 2395, InvivoGen), which stimulates pDCs and B cells [ ], was intranasally administered to each group of mice every 2 days for 3 weeks.

Techniques: Expressing, Gene Expression

Ratio of Gd-IgA1/IgA levels in culture supernatants of tonsillar MNCs from patients with IgAN correlates with the percentage of pDCs and the expression of APRIL in tonsillar MNCs. Association between Gd-IgA1/IgA levels and tonsillar pDC frequency or gene expression in MNCs from patients with IgAN ( n = 7, red dots) and CT ( n = 8, black dots). ( A ) The ratios of Gd-IgA1/IgA in culture supernatants of tonsillar MNCs from patients with IgAN or CT. ( B ) Correlation between the Gd-IgA1/IgA ratio and the percentage of tonsillar pDCs in MNCs from patients with IgAN and CT. ( C ) Correlation between the Gd-IgA1/IgA ratio and the expression of APRIL in MNCs from patients with IgAN and CT. ( D ) Correlation between the Gd-IgA1/IgA ratio and the expression of BAFF in MNCs from patients with IgAN and CT. ( E ) Association between the APRIL and TLR9 expression levels in tonsillar MNCs from patients with IgAN and CT. Correlations were assessed using Spearman’s correlation coefficients (r) with P -values shown in each panel. ns, not significant; * P ≤ .05.

Journal: Nephrology Dialysis Transplantation

Article Title: Plasmacytoid dendritic cells modulate the production of mucosal IgA in IgA nephropathy

doi: 10.1093/ndt/gfaf216

Figure Lengend Snippet: Ratio of Gd-IgA1/IgA levels in culture supernatants of tonsillar MNCs from patients with IgAN correlates with the percentage of pDCs and the expression of APRIL in tonsillar MNCs. Association between Gd-IgA1/IgA levels and tonsillar pDC frequency or gene expression in MNCs from patients with IgAN ( n = 7, red dots) and CT ( n = 8, black dots). ( A ) The ratios of Gd-IgA1/IgA in culture supernatants of tonsillar MNCs from patients with IgAN or CT. ( B ) Correlation between the Gd-IgA1/IgA ratio and the percentage of tonsillar pDCs in MNCs from patients with IgAN and CT. ( C ) Correlation between the Gd-IgA1/IgA ratio and the expression of APRIL in MNCs from patients with IgAN and CT. ( D ) Correlation between the Gd-IgA1/IgA ratio and the expression of BAFF in MNCs from patients with IgAN and CT. ( E ) Association between the APRIL and TLR9 expression levels in tonsillar MNCs from patients with IgAN and CT. Correlations were assessed using Spearman’s correlation coefficients (r) with P -values shown in each panel. ns, not significant; * P ≤ .05.

Article Snippet: The TLR9 agonist class C CpG-ODN (ODN 2395, InvivoGen), which stimulates pDCs and B cells [ ], was intranasally administered to each group of mice every 2 days for 3 weeks.

Techniques: Expressing, Gene Expression