counterstaining with dapi (Beyotime)
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counterstaining with dapi
Counterstaining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/counterstaining+with+dapi/DAPI/pm41981465-107-32-35
Average 99 stars, based on 32308 article reviews
Counterstaining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/counterstaining+with+dapi/DAPI/pm41981465-107-32-35
Average 99 stars, based on 32308 article reviews
counterstaining with dapi - by Bioz Stars,
2026-10
99/100 stars
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Blocking Assay:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and Incubation:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: Spatially Defined DNA Origami Cell Engagers for T and Natural Killer Cell-Mediated Immune Modulation. Article Snippet: Cell engagers have emerged as a promising approach for cancer immunotherapy, yet their efficacy is often limited by structural constraints in antibody valency and spatial configuration.. Here, we present a DNA origamibased platform for the precise spatial organization of antibodies, enabling the construction of tailored multivalent cell engagers to enhance antitumor immunity.. By modulating the composition, valency, and spatial arrangement of antibodies on a single DNA origami scaffold at the single-molecule level, we developed two types of cell engagers, T cell engagers and natural killer cell engagers, that effectively activate immune cells and improve tumor specificity. Article Title: GDPD5-CD55-EGFR competitive binding axis regulates radioresistance and lipid accumulation in rectal cancer. Article Snippet: .. After PBS washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific) for lipid droplet visualization, followed by nuclear Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and Article Title: Elucidating the Role of Oxidative Stress-Associated Genes FKBP Prolyl Isomerase 5 in Osteoarthritis Development and Immunological Milieu. Article Snippet: After removing the culture medium, cells were fixed with 4% paraformaldehyde (Biosharp, Hefei, China) for 10 minutes at ambient temperature and then rinsed three times with PBS (Gibco, Grand Island, NY, USA). .. The EdU detection procedure was conducted according to manufacturer specifications using the Click-iTTM EdU Imaging Kit (Invitrogen, Carlsbad, CA, USA): cells were incubated with EdU reaction mixture (light-protected environment) followed by nuclear Fluorescence:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: Photothermal reprogramming of synovial M1 macrophages reshapes the pro-inflammatory microenvironment to reverse temporomandibular joint osteoarthritis Article Snippet: .. Nuclei were visualized by Article Title: Extracellular Vesicle‐Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood‐Tumour Barrier Formation Article Snippet: .. After Microscopy:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: Extracellular Vesicle‐Mediated Nucleolin Transfer in Glioblastoma: A Targetable Axis Driving Blood‐Tumour Barrier Formation Article Snippet: .. After Laser-Scanning Microscopy:Article Title: Photothermal reprogramming of synovial M1 macrophages reshapes the pro-inflammatory microenvironment to reverse temporomandibular joint osteoarthritis Article Snippet: .. Nuclei were visualized by Confocal Laser Scanning Microscopy:Article Title: Photothermal reprogramming of synovial M1 macrophages reshapes the pro-inflammatory microenvironment to reverse temporomandibular joint osteoarthritis Article Snippet: .. Nuclei were visualized by Immunofluorescence:Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and Staining:Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and Imaging:Article Title: Elucidating the Role of Oxidative Stress-Associated Genes FKBP Prolyl Isomerase 5 in Osteoarthritis Development and Immunological Milieu. Article Snippet: After removing the culture medium, cells were fixed with 4% paraformaldehyde (Biosharp, Hefei, China) for 10 minutes at ambient temperature and then rinsed three times with PBS (Gibco, Grand Island, NY, USA). .. The EdU detection procedure was conducted according to manufacturer specifications using the Click-iTTM EdU Imaging Kit (Invitrogen, Carlsbad, CA, USA): cells were incubated with EdU reaction mixture (light-protected environment) followed by nuclear |
