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corticosterone  (MedChemExpress)


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    MedChemExpress corticosterone
    Corticosterone, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bezafibrate alleviates neuroinflammation and enhances phagocytosis in an NLRP6-dependent manner. (A-C) Schematic diagrams and quantitative results of the OFT (A), FST (B) and TST (C) behavioral experiment (n = 10). (D) SPT for assessing anhedonia-like behavior (n = 10). (E) Serum CORT levels detected by <t>ELISA</t> (n = 8). (F) qPCR analysis of Nlrp6 mRNA expression levels in the hippocampus of mice. (G) Representative Western blots images and quantitative analysis (relative to GAPDH) of CD36 protein expression levels in the hippocampus of mice (n = 4). (H) Representative immunofluorescence images of fluorescent microscopy showing cellular debris and microglia (Iba1) in brain slices of mice. (I) Quantification of cellular debris fluorescence intensity (n = 4). (J) qPCR analysis of Il-6 , Tnf-α , Il-10 and Il-4 mRNA expression levels in the hippocampus of mice (n = 5). CTL: control; Bez: bezafibrate; Nlrp6 -KO: NLRP6 complete gene knockout. CRS: chronic restraint stress. Data are presented as mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant.
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    Bezafibrate alleviates neuroinflammation and enhances phagocytosis in an NLRP6-dependent manner. (A-C) Schematic diagrams and quantitative results of the OFT (A), FST (B) and TST (C) behavioral experiment (n = 10). (D) SPT for assessing anhedonia-like behavior (n = 10). (E) Serum CORT levels detected by ELISA (n = 8). (F) qPCR analysis of Nlrp6 mRNA expression levels in the hippocampus of mice. (G) Representative Western blots images and quantitative analysis (relative to GAPDH) of CD36 protein expression levels in the hippocampus of mice (n = 4). (H) Representative immunofluorescence images of fluorescent microscopy showing cellular debris and microglia (Iba1) in brain slices of mice. (I) Quantification of cellular debris fluorescence intensity (n = 4). (J) qPCR analysis of Il-6 , Tnf-α , Il-10 and Il-4 mRNA expression levels in the hippocampus of mice (n = 5). CTL: control; Bez: bezafibrate; Nlrp6 -KO: NLRP6 complete gene knockout. CRS: chronic restraint stress. Data are presented as mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant.

    Journal: Journal of Advanced Research

    Article Title: NLRP6 governs hippocampal microglial phagocytosis to resist stress-induced depressive-like behaviors in mice and the intervention effect of bezafibrate

    doi: 10.1016/j.jare.2025.08.050

    Figure Lengend Snippet: Bezafibrate alleviates neuroinflammation and enhances phagocytosis in an NLRP6-dependent manner. (A-C) Schematic diagrams and quantitative results of the OFT (A), FST (B) and TST (C) behavioral experiment (n = 10). (D) SPT for assessing anhedonia-like behavior (n = 10). (E) Serum CORT levels detected by ELISA (n = 8). (F) qPCR analysis of Nlrp6 mRNA expression levels in the hippocampus of mice. (G) Representative Western blots images and quantitative analysis (relative to GAPDH) of CD36 protein expression levels in the hippocampus of mice (n = 4). (H) Representative immunofluorescence images of fluorescent microscopy showing cellular debris and microglia (Iba1) in brain slices of mice. (I) Quantification of cellular debris fluorescence intensity (n = 4). (J) qPCR analysis of Il-6 , Tnf-α , Il-10 and Il-4 mRNA expression levels in the hippocampus of mice (n = 5). CTL: control; Bez: bezafibrate; Nlrp6 -KO: NLRP6 complete gene knockout. CRS: chronic restraint stress. Data are presented as mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant.

    Article Snippet: The concentrations of CORT in the serum were measured using enzyme-linked immunosorbent assay (ELISA) kits (KGE009, R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Immunofluorescence, Microscopy, Fluorescence, Control, Gene Knockout