Journal: Bioactive Materials

Article Title: Precisely regulated physically-crosslinked carriers enable synergetic release of bioactive factors for MSC-mediated cartilage regeneration

doi: 10.1016/j.bioactmat.2026.01.009

Figure Lengend Snippet: Characterization of microspheres. (a) Scanning electronic microscopic (SEM) observations shows the morphology and surface structure of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h. (b) Fourier transform infrared spectroscopy (FTIR) analysis showing the different second structure of SF (β-sheet, random coil/helix and β-turn) content in different recrystallization time. (c) Matched curve of β-sheet fraction with assembly time. Illustrations showed the morphology of SF microspheres with recrystallization (assembly) time (t) of 0 h, 24 h and 48 h, as well as the Fourier transform infrared spectroscopy (FTIR) analysis showing the change of β-sheet content in different recrystallization time. (d, e) Cumulative release curve of MAP and KGN from PSF and KSF with different β-sheet fraction respectively. (f) The contact angle with water of SF with different β-sheet fraction showing the wetting property. (g) The degradation of SF with different β-sheet fraction. (h) Comparison of release kinetics for drug releasing strategies between our approach (red line) and previous studies reported . The illustration showed the comparison of release kinetics for MSC recruiting strategies between our approach (gray area) and previous studies (purple points). (i) In vivo retention of PSF microspheres injected in the joint cavity visualized by in vivo imaging system (IVIS). (j) Fluorescent intensity (Taking the base-10 logarithm) at different timepoint after injection. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: Immunofluorescent staining was performed with Aggrecan primary antibody (13880-1-AP, Proteintech, USA), ActinGreen ( R37110 , Thermo, USA) and DAPI (Solarbio, China) and observed with 3D reconstruction under high content imaging system (PerkinElmer, Operetta CLS, USA).

Techniques: Recrystallization, Fourier Transform Infrared Spectroscopy, Spectroscopy, Comparison, In Vivo, Injection, In Vivo Imaging

Journal: Translational Oncology

Article Title: Overcoming the leptomeningeal seeding of medulloblastoma by targeting HSP70

doi: 10.1016/j.tranon.2026.102695

Figure Lengend Snippet: Phenotypic differences between LMS-derived seeding (S3) and non-seeding (N3) cells. (A) Morphological differences were not observed between S3 and N3 under bright field microscope. (B) The graph shows that the proliferative capacity of S3 is slower than that of N3s through High-Content Screening (HCS) analysis. (C) The trans-well migration assay reveals that there was no significant difference in the migration ability between S3 and N3 (quantification graph). (D) Representative images from the trans-well migration assay. (E) Representative images of the wound-healing assay show that N3 filled the open area faster than S3. The white area is saturated with green color due to the overlapping of S3 without filling the wound gap. (F) Quantification graph of the wound-healing assay. (G) Adhesion of S3 and N3 cells to extracellular matrix (ECM) components, including fibronectin, collagen I, collagen IV, laminin I, and fibrinogen, was quantified after 24h of incubation. (H) Adhesion values were normalized to control conditions. All data are presented as mean ± SD from n = 3 independent experiments. Statistical comparisons between two groups were performed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Cell proliferation was monitored and quantified consecutively for 64 hours using a high-content screening (HCS) system (Operetta CLS, PerkinElmer).

Techniques: Derivative Assay, Microscopy, High Content Screening, Migration, Wound Healing Assay, Incubation, Control, Two Tailed Test