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opera phenix high content screening microscope  (Revvity)
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Revvity opera phenix high content screening microscope
Opera Phenix High Content Screening Microscope, supplied by Revvity, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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opera phenix high content screening system  (Revvity)
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Revvity opera phenix high content screening system
Opera Phenix High Content Screening System, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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operetta high content screening system  (Revvity)
99
Revvity operetta high content screening system
Operetta High Content Screening System, supplied by Revvity, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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operetta high content screening system - by Bioz Stars, 2026-03
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operetta clstm high content analysis system  (Revvity)
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Revvity operetta clstm high content analysis system
Operetta Clstm High Content Analysis System, supplied by Revvity, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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toluidine blue stain  (Chem Impex International)
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Chem Impex International toluidine blue stain
Toluidine Blue Stain, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagexpress micro 4  (Danaher Inc)
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Danaher Inc imagexpress micro 4
Imagexpress Micro 4, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 methyl2 pyrrolidinone  (Chem Impex International)
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Chem Impex International 1 methyl2 pyrrolidinone
1 Methyl2 Pyrrolidinone, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagexpress micro confocal  (Danaher Inc)
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Danaher Inc imagexpress micro confocal
Imagexpress Micro Confocal, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagexpress micro confocal highcontent imaging system  (Danaher Inc)
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Danaher Inc imagexpress micro confocal highcontent imaging system
Imagexpress Micro Confocal Highcontent Imaging System, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytoplasm  (Revvity)
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Revvity cytoplasm
Cytoplasm, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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operetta cls system  (Revvity)
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Revvity operetta cls system
GlyPerA™ shields primary HAE cells from SARS-CoV-2 Omicron BA.1 and BA.2 infection and innate immune activation. Visualization of virus binding (SARS-CoV-2 S1/N, orange) and complement (C3-FITC, green) in SARS-CoV-2 infected 3D pseudostratified epithelia. Pseudostratified epithelia were apically treated with GlyPerA™ prior exposure to SARS-CoV-2. On 3 dpi, filters were fixed, stained for höchst (blue), SARS-CoV-2 S1/N (orange), complement C3 (green) and acetylated tubulin (red) and then analysed by HCS. a XYZ-stacks of uninfected (UI, panel 1), BA.2-infected (panel 2), GlyPerA™-pre-treated and BA.2-infected (panel 3) HAE cultures were analyzed using <t>the</t> <t>Operetta</t> <t>CLS</t> HCS and the 63XWATER objective. Cells were stained using C3-FITC (green) as indicator for innate immune activation, SARS-CoV-2-S1/N-Alexa594 (orange) for virus detection, höchst for imaging nuclei (blue) and acetylated tubulin for staining cilia (red). High IC C3 mobilization was monitored in BA.2-infected cultures, while no virus and low C3 signals were detected in UI (panel 1) and GlyPerA™/BA.2- (panel 3) cultures. Scale bars represent 50 µm (XY) and 20 µm (YZ, XZ), respectively. Three independent experiments were performed. b Numbers of nuclei per condition (UI, Omicron, GlyPerA dil. PRE Omicron, GlyPerA dil. SIM Omicron), viral signals and c areas of C3 production were absolutely quantified from at least three different areas using the Harmony™ 4.9 software. Statistical significances were analyzed with GraphPad Prism software using One-way ANOVA and Tukey’s post test. In b and c **P < 0.01, ***P < 0.001, ****P < 0.0001
Operetta Cls System, supplied by Revvity, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
operetta cls system - by Bioz Stars, 2026-03
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5 6 carboxyfluorescein  (Chem Impex International)
95
Chem Impex International 5 6 carboxyfluorescein
GlyPerA™ shields primary HAE cells from SARS-CoV-2 Omicron BA.1 and BA.2 infection and innate immune activation. Visualization of virus binding (SARS-CoV-2 S1/N, orange) and complement (C3-FITC, green) in SARS-CoV-2 infected 3D pseudostratified epithelia. Pseudostratified epithelia were apically treated with GlyPerA™ prior exposure to SARS-CoV-2. On 3 dpi, filters were fixed, stained for höchst (blue), SARS-CoV-2 S1/N (orange), complement C3 (green) and acetylated tubulin (red) and then analysed by HCS. a XYZ-stacks of uninfected (UI, panel 1), BA.2-infected (panel 2), GlyPerA™-pre-treated and BA.2-infected (panel 3) HAE cultures were analyzed using <t>the</t> <t>Operetta</t> <t>CLS</t> HCS and the 63XWATER objective. Cells were stained using C3-FITC (green) as indicator for innate immune activation, SARS-CoV-2-S1/N-Alexa594 (orange) for virus detection, höchst for imaging nuclei (blue) and acetylated tubulin for staining cilia (red). High IC C3 mobilization was monitored in BA.2-infected cultures, while no virus and low C3 signals were detected in UI (panel 1) and GlyPerA™/BA.2- (panel 3) cultures. Scale bars represent 50 µm (XY) and 20 µm (YZ, XZ), respectively. Three independent experiments were performed. b Numbers of nuclei per condition (UI, Omicron, GlyPerA dil. PRE Omicron, GlyPerA dil. SIM Omicron), viral signals and c areas of C3 production were absolutely quantified from at least three different areas using the Harmony™ 4.9 software. Statistical significances were analyzed with GraphPad Prism software using One-way ANOVA and Tukey’s post test. In b and c **P < 0.01, ***P < 0.001, ****P < 0.0001
5 6 Carboxyfluorescein, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 6 carboxyfluorescein/product/Chem Impex International
Average 95 stars, based on 1 article reviews
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Image Search Results


GlyPerA™ shields primary HAE cells from SARS-CoV-2 Omicron BA.1 and BA.2 infection and innate immune activation. Visualization of virus binding (SARS-CoV-2 S1/N, orange) and complement (C3-FITC, green) in SARS-CoV-2 infected 3D pseudostratified epithelia. Pseudostratified epithelia were apically treated with GlyPerA™ prior exposure to SARS-CoV-2. On 3 dpi, filters were fixed, stained for höchst (blue), SARS-CoV-2 S1/N (orange), complement C3 (green) and acetylated tubulin (red) and then analysed by HCS. a XYZ-stacks of uninfected (UI, panel 1), BA.2-infected (panel 2), GlyPerA™-pre-treated and BA.2-infected (panel 3) HAE cultures were analyzed using the Operetta CLS HCS and the 63XWATER objective. Cells were stained using C3-FITC (green) as indicator for innate immune activation, SARS-CoV-2-S1/N-Alexa594 (orange) for virus detection, höchst for imaging nuclei (blue) and acetylated tubulin for staining cilia (red). High IC C3 mobilization was monitored in BA.2-infected cultures, while no virus and low C3 signals were detected in UI (panel 1) and GlyPerA™/BA.2- (panel 3) cultures. Scale bars represent 50 µm (XY) and 20 µm (YZ, XZ), respectively. Three independent experiments were performed. b Numbers of nuclei per condition (UI, Omicron, GlyPerA dil. PRE Omicron, GlyPerA dil. SIM Omicron), viral signals and c areas of C3 production were absolutely quantified from at least three different areas using the Harmony™ 4.9 software. Statistical significances were analyzed with GraphPad Prism software using One-way ANOVA and Tukey’s post test. In b and c **P < 0.01, ***P < 0.001, ****P < 0.0001

Journal: Respiratory Research

Article Title: GlyPerA™ effectively shields airway epithelia from SARS-CoV-2 infection and inflammatory events

doi: 10.1186/s12931-023-02397-3

Figure Lengend Snippet: GlyPerA™ shields primary HAE cells from SARS-CoV-2 Omicron BA.1 and BA.2 infection and innate immune activation. Visualization of virus binding (SARS-CoV-2 S1/N, orange) and complement (C3-FITC, green) in SARS-CoV-2 infected 3D pseudostratified epithelia. Pseudostratified epithelia were apically treated with GlyPerA™ prior exposure to SARS-CoV-2. On 3 dpi, filters were fixed, stained for höchst (blue), SARS-CoV-2 S1/N (orange), complement C3 (green) and acetylated tubulin (red) and then analysed by HCS. a XYZ-stacks of uninfected (UI, panel 1), BA.2-infected (panel 2), GlyPerA™-pre-treated and BA.2-infected (panel 3) HAE cultures were analyzed using the Operetta CLS HCS and the 63XWATER objective. Cells were stained using C3-FITC (green) as indicator for innate immune activation, SARS-CoV-2-S1/N-Alexa594 (orange) for virus detection, höchst for imaging nuclei (blue) and acetylated tubulin for staining cilia (red). High IC C3 mobilization was monitored in BA.2-infected cultures, while no virus and low C3 signals were detected in UI (panel 1) and GlyPerA™/BA.2- (panel 3) cultures. Scale bars represent 50 µm (XY) and 20 µm (YZ, XZ), respectively. Three independent experiments were performed. b Numbers of nuclei per condition (UI, Omicron, GlyPerA dil. PRE Omicron, GlyPerA dil. SIM Omicron), viral signals and c areas of C3 production were absolutely quantified from at least three different areas using the Harmony™ 4.9 software. Statistical significances were analyzed with GraphPad Prism software using One-way ANOVA and Tukey’s post test. In b and c **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: To study these complex models using primary cell cultured in 3D and to generate detailed phenotypic fingerprints for deeper biological insights in a high throughput manner, the Operetta CLS System (PerkinElmer, Waltham, MA, USA) was applied.

Techniques: Infection, Activation Assay, Virus, Binding Assay, Staining, Imaging, Software