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constructs encoding pum1  (OriGene)


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    Structured Review

    OriGene constructs encoding pum1
    Figure 3: <t>PUM1</t> and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.
    Constructs Encoding Pum1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constructs+encoding+pum1/Pumilio+1+(PUM1)+(NM_014676)+Human+Tagged+ORF+Clone/pm30197756-194-0-22
    Average 90 stars, based on 5 article reviews
    constructs encoding pum1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma."

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

    Journal: Oncotarget

    doi: 10.18632/oncotarget.25977

    Figure 3: PUM1 and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.
    Figure Legend Snippet: Figure 3: PUM1 and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.

    Techniques Used: Binding Assay, Luciferase, Quantitative RT-PCR, Negative Control, Knockdown, Isolation, Expressing, Over Expression, Western Blot

    Figure 4: Influence of PUM1 and PUM2 proteins on luciferase reporter constructs carrying SPIN1 or SPIN3 3ʹUTRs. The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3ʹUTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) (A). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3ʹUTR, which lacks PBE motifs (negative control) (B). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3ʹUTRs (C). Effects of PUM1 or PUM2 overexpression (D). or knockdown (E). on luciferase constructs containing short SPIN1 or SPIN3 3ʹUTR fragments. **P ≤ 0.005, ***P ≤ 0.0005, ****P ≤ 0.00005.
    Figure Legend Snippet: Figure 4: Influence of PUM1 and PUM2 proteins on luciferase reporter constructs carrying SPIN1 or SPIN3 3ʹUTRs. The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3ʹUTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) (A). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3ʹUTR, which lacks PBE motifs (negative control) (B). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3ʹUTRs (C). Effects of PUM1 or PUM2 overexpression (D). or knockdown (E). on luciferase constructs containing short SPIN1 or SPIN3 3ʹUTR fragments. **P ≤ 0.005, ***P ≤ 0.0005, ****P ≤ 0.00005.

    Techniques Used: Luciferase, Construct, Expressing, Over Expression, Plasmid Preparation, Negative Control, Knockdown

    Figure 5: PUM1 induces TCam-2 cell apoptosis. TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs (A). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector (B), PUM1 (C), or PUM2 (D) constructs.
    Figure Legend Snippet: Figure 5: PUM1 induces TCam-2 cell apoptosis. TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs (A). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector (B), PUM1 (C), or PUM2 (D) constructs.

    Techniques Used: Transfection, Construct, Plasmid Preparation, Cell Culture

    Figure 6: PUM1 and PUM2 slightly downregulate TCam-2 cell cycle progression. PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.
    Figure Legend Snippet: Figure 6: PUM1 and PUM2 slightly downregulate TCam-2 cell cycle progression. PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.

    Techniques Used:

    Related Articles

    Construct:

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.
    Article Snippet: .. Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies. .. For luciferase assays, the full-length GAPDH (201 nt) SPIN1 (3429 nt), or SPIN3 (3339 nt) 3ʹUTRs or their fragments, SPIN1 (195 nt) and SPIN3 (218 nt), were cloned into the psiCheck2 vector (Promega) with the Renilla luciferase ORF, using NotI and XhoI restriction sites.

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma
    Article Snippet: .. Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies. .. For luciferase assays, the full-length GAPDH (201 nt) SPIN1 (3429 nt), or SPIN3 (3339 nt) 3′UTRs or their fragments, SPIN1 (195 nt) and SPIN3 (218 nt), were cloned into the psiCheck2 vector (Promega) with the Renilla luciferase ORF, using NotI and XhoI restriction sites.

    Plasmid Preparation:

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.
    Article Snippet: .. Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies. .. For luciferase assays, the full-length GAPDH (201 nt) SPIN1 (3429 nt), or SPIN3 (3339 nt) 3ʹUTRs or their fragments, SPIN1 (195 nt) and SPIN3 (218 nt), were cloned into the psiCheck2 vector (Promega) with the Renilla luciferase ORF, using NotI and XhoI restriction sites.

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma
    Article Snippet: .. Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies. .. For luciferase assays, the full-length GAPDH (201 nt) SPIN1 (3429 nt), or SPIN3 (3339 nt) 3′UTRs or their fragments, SPIN1 (195 nt) and SPIN3 (218 nt), were cloned into the psiCheck2 vector (Promega) with the Renilla luciferase ORF, using NotI and XhoI restriction sites.

    Over Expression:

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.
    Article Snippet: .. Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies. .. For luciferase assays, the full-length GAPDH (201 nt) SPIN1 (3429 nt), or SPIN3 (3339 nt) 3ʹUTRs or their fragments, SPIN1 (195 nt) and SPIN3 (218 nt), were cloned into the psiCheck2 vector (Promega) with the Renilla luciferase ORF, using NotI and XhoI restriction sites.

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma
    Article Snippet: .. Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies. .. For luciferase assays, the full-length GAPDH (201 nt) SPIN1 (3429 nt), or SPIN3 (3339 nt) 3′UTRs or their fragments, SPIN1 (195 nt) and SPIN3 (218 nt), were cloned into the psiCheck2 vector (Promega) with the Renilla luciferase ORF, using NotI and XhoI restriction sites.



    Similar Products

    constructs encoding pum1  (OriGene)
    90
    OriGene constructs encoding pum1
    Figure 3: <t>PUM1</t> and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.
    Constructs Encoding Pum1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constructs+encoding+pum1/Pumilio+1+(PUM1)+(NM_014676)+Human+Tagged+ORF+Clone/pm30197756-194-0-22
    Average 90 stars, based on 1 article reviews
    constructs encoding pum1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 3: PUM1 and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.

    Journal: Oncotarget

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

    doi: 10.18632/oncotarget.25977

    Figure Lengend Snippet: Figure 3: PUM1 and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.

    Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

    Techniques: Binding Assay, Luciferase, Quantitative RT-PCR, Negative Control, Knockdown, Isolation, Expressing, Over Expression, Western Blot

    Figure 4: Influence of PUM1 and PUM2 proteins on luciferase reporter constructs carrying SPIN1 or SPIN3 3ʹUTRs. The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3ʹUTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) (A). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3ʹUTR, which lacks PBE motifs (negative control) (B). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3ʹUTRs (C). Effects of PUM1 or PUM2 overexpression (D). or knockdown (E). on luciferase constructs containing short SPIN1 or SPIN3 3ʹUTR fragments. **P ≤ 0.005, ***P ≤ 0.0005, ****P ≤ 0.00005.

    Journal: Oncotarget

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

    doi: 10.18632/oncotarget.25977

    Figure Lengend Snippet: Figure 4: Influence of PUM1 and PUM2 proteins on luciferase reporter constructs carrying SPIN1 or SPIN3 3ʹUTRs. The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3ʹUTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) (A). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3ʹUTR, which lacks PBE motifs (negative control) (B). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3ʹUTRs (C). Effects of PUM1 or PUM2 overexpression (D). or knockdown (E). on luciferase constructs containing short SPIN1 or SPIN3 3ʹUTR fragments. **P ≤ 0.005, ***P ≤ 0.0005, ****P ≤ 0.00005.

    Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

    Techniques: Luciferase, Construct, Expressing, Over Expression, Plasmid Preparation, Negative Control, Knockdown

    Figure 5: PUM1 induces TCam-2 cell apoptosis. TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs (A). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector (B), PUM1 (C), or PUM2 (D) constructs.

    Journal: Oncotarget

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

    doi: 10.18632/oncotarget.25977

    Figure Lengend Snippet: Figure 5: PUM1 induces TCam-2 cell apoptosis. TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs (A). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector (B), PUM1 (C), or PUM2 (D) constructs.

    Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

    Techniques: Transfection, Construct, Plasmid Preparation, Cell Culture

    Figure 6: PUM1 and PUM2 slightly downregulate TCam-2 cell cycle progression. PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.

    Journal: Oncotarget

    Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

    doi: 10.18632/oncotarget.25977

    Figure Lengend Snippet: Figure 6: PUM1 and PUM2 slightly downregulate TCam-2 cell cycle progression. PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.

    Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

    Techniques: