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recombinant lentiviral constructs encoding fatty acid-binding protein (fabp4), fabp4-rnai and gfp control particles  (Shanghai Genechem Ltd)

 
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    Shanghai Genechem Ltd recombinant lentiviral constructs encoding fatty acid-binding protein (fabp4), fabp4-rnai and gfp control particles
    Recombinant Lentiviral Constructs Encoding Fatty Acid Binding Protein (Fabp4), Fabp4 Rnai And Gfp Control Particles, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/construct+encoding+gfp/recombinant+lentiviral+constructs+encoding+fatty+acid+binding+protein++fabp4+++fabp4+rnai+and+gfp+control+particles/pm40532836-131-5-16
    Average 90 stars, based on 1 article reviews
    recombinant lentiviral constructs encoding fatty acid-binding protein (fabp4), fabp4-rnai and gfp control particles - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Propranolol induces apoptosis of human umbilical vein endothelial cells through downregulation of CD147.
    Article Snippet: Propranolol induces apoptosis of human umbilical vein endothelial cells through downregulation of CD147 W. Xie, H. Xie, F. Liu, W. Li, J. Dan, Y. Mei, L. Dan, X. Xiao, J. Li and X. Chen Department of Dermatology, Xiangya Hospital, Central South University, Changsha, 410008, China Department of Dermatology, Mawangdui Hospital, Changsha, Hunan 410016, China Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 41008, China The State Key Laboratory of Medical Genetics, Institute of Medical Genetics, Central South University, Changsha, Hunan, 41008, China

    shRNA:

    Article Title: Role and mechanism of the Dectin-1-mediated Syk/NF-κB signaling pathway in Talaromyces marneffei infection
    Article Snippet: One Step TB GreenTM Prime ScriptTM RT-PCR kit II was purchased from Takara Bio, Inc. RIPA lysis buffer was purchased from Beijing Solarbio Science & Technology Co., Ltd. BSA, BCA assay and blocking buffer were purchased from Beyotime Institute of Biotechnology. .. ECL was purchased from Bio-Rad Laboratories, Inc. Dectin-1 short hairpin (sh)RNA lentiviral particles and scramble shRNA lentiviral particles encoding a GFP sequence were constructed by Shanghai GeneChem Co., Ltd. Antibodies against Dectin-1 (cat. no. 60128), NF-κB p65 (cat. no. 8242), phosphorylated (p)-NF-κB p65 (cat. no. 3033), IκBα (cat. no. 4814S), p-IκBα (cat. no. 9246), Syk (cat. no. 2712), p-Syk (cat. no. 2710) and anti-rabbit IgG (H + L), Alexa Fluor ® 555-conjugated anti-rabbit IgG (cat. no. 4413) were purchased from Cell Signaling Technology, Inc.; horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (cat. no. L3012 was purchased from Signalway Antibody LLC; HRP-conjugated goat anti-mouse IgG secondary antibody (cat. no. ab6789) was purchased from Abcam; and anti-β-actin (cat. no. 66009-1-Ig) was purchased from ProteinTech Group, Inc. ..

    Article Title: B7-H3 combats apoptosis induced by chemotherapy by delivering signals to pancreatic cancer cells
    Article Snippet: .. shRNA of the human B7-H3 (5'-CTCTGCCTTCTCACCTCTTTG-3') and overexpressing hB7-H3 of lentivirus gene transfer vectors encoding the GFP sequence were constructed by Shanghai Genechem Co. (Shanghai, China). ..

    Sequencing:

    Article Title: Role and mechanism of the Dectin-1-mediated Syk/NF-κB signaling pathway in Talaromyces marneffei infection
    Article Snippet: One Step TB GreenTM Prime ScriptTM RT-PCR kit II was purchased from Takara Bio, Inc. RIPA lysis buffer was purchased from Beijing Solarbio Science & Technology Co., Ltd. BSA, BCA assay and blocking buffer were purchased from Beyotime Institute of Biotechnology. .. ECL was purchased from Bio-Rad Laboratories, Inc. Dectin-1 short hairpin (sh)RNA lentiviral particles and scramble shRNA lentiviral particles encoding a GFP sequence were constructed by Shanghai GeneChem Co., Ltd. Antibodies against Dectin-1 (cat. no. 60128), NF-κB p65 (cat. no. 8242), phosphorylated (p)-NF-κB p65 (cat. no. 3033), IκBα (cat. no. 4814S), p-IκBα (cat. no. 9246), Syk (cat. no. 2712), p-Syk (cat. no. 2710) and anti-rabbit IgG (H + L), Alexa Fluor ® 555-conjugated anti-rabbit IgG (cat. no. 4413) were purchased from Cell Signaling Technology, Inc.; horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (cat. no. L3012 was purchased from Signalway Antibody LLC; HRP-conjugated goat anti-mouse IgG secondary antibody (cat. no. ab6789) was purchased from Abcam; and anti-β-actin (cat. no. 66009-1-Ig) was purchased from ProteinTech Group, Inc. ..

    Article Title: B7-H3 combats apoptosis induced by chemotherapy by delivering signals to pancreatic cancer cells
    Article Snippet: .. shRNA of the human B7-H3 (5'-CTCTGCCTTCTCACCTCTTTG-3') and overexpressing hB7-H3 of lentivirus gene transfer vectors encoding the GFP sequence were constructed by Shanghai Genechem Co. (Shanghai, China). ..

    Construct:

    Article Title: Role and mechanism of the Dectin-1-mediated Syk/NF-κB signaling pathway in Talaromyces marneffei infection
    Article Snippet: One Step TB GreenTM Prime ScriptTM RT-PCR kit II was purchased from Takara Bio, Inc. RIPA lysis buffer was purchased from Beijing Solarbio Science & Technology Co., Ltd. BSA, BCA assay and blocking buffer were purchased from Beyotime Institute of Biotechnology. .. ECL was purchased from Bio-Rad Laboratories, Inc. Dectin-1 short hairpin (sh)RNA lentiviral particles and scramble shRNA lentiviral particles encoding a GFP sequence were constructed by Shanghai GeneChem Co., Ltd. Antibodies against Dectin-1 (cat. no. 60128), NF-κB p65 (cat. no. 8242), phosphorylated (p)-NF-κB p65 (cat. no. 3033), IκBα (cat. no. 4814S), p-IκBα (cat. no. 9246), Syk (cat. no. 2712), p-Syk (cat. no. 2710) and anti-rabbit IgG (H + L), Alexa Fluor ® 555-conjugated anti-rabbit IgG (cat. no. 4413) were purchased from Cell Signaling Technology, Inc.; horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (cat. no. L3012 was purchased from Signalway Antibody LLC; HRP-conjugated goat anti-mouse IgG secondary antibody (cat. no. ab6789) was purchased from Abcam; and anti-β-actin (cat. no. 66009-1-Ig) was purchased from ProteinTech Group, Inc. ..

    Article Title: B7-H3 combats apoptosis induced by chemotherapy by delivering signals to pancreatic cancer cells
    Article Snippet: .. shRNA of the human B7-H3 (5'-CTCTGCCTTCTCACCTCTTTG-3') and overexpressing hB7-H3 of lentivirus gene transfer vectors encoding the GFP sequence were constructed by Shanghai Genechem Co. (Shanghai, China). ..



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    Addgene inc dna construct encoding gfp cortactin
    (a) Representative images of day 6 neurons after Lysotracker treatment. Lysotracker is shown in magenta, and DAPI in blue. Scale bar = 20 μm. (b) Quantification of Lysotracker signal in day 6 neurons. The relative Lysotracker intensity of CLCN4 -variant neurons compared to the WT was presented. Each dot represents one biological replicate. (n=3-4, mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test * p =0.039 for variant A; *** p =0.0007 for variant B; ** p =0.0092 for variant C) The magenta dashed line indicates the value for WT. (c) Relative acidity in acidic <t>subcellular</t> organelles. Each dot represents one biological replicate. (n=3, mean ± SEM, unpaired Student’s t-test; p =0.2894) (d-g) Dynamics of endo-lysosomal vesicles. RAB5, RAB7, RAB11 and LAMP1 indicates early endosome, late endosome, recycling endosome and lysosome markers, respectively. (RAB5, n=6; RAB7, n=3; RAB11, n=4; and LAMP1, n=3 or 4; mean ± SEM, unpaired Student’s t-test) (d) Representative images of a neuron with GFP-labeled vesicular organelles. TUJ1 and DAPI are shown in red and blue, respectively. Scale bar = 10 μm. (e) Relative GFP intensity expressed in vesicular organelles (* p =0.0265 for RAB5, * p =0.0158 for RAB7, * p =0.0475 for RAB11, and ** p =0.0067 for LAMP1) (f) Number of GFP-expressing vesicles in neurons. (* p =0.0186 for RAB5, * *p =0.0066 for RAB7, and p =0.9031 for LAMP1) (g) Average distance of GFP-expressing vesicles from the center of nucleus, representing the mean linear distance of puncta from the center of the nucleus in each cell. (** p =0.0011 for RAB5, * p =0.0463 for RAB7, and * p =0.0103 for LAMP1) (h) Scheme of autophagic flux analysis using tandem fluorescent protein fused to LC3. (i) Representative images of day 6 neurons expressing tandem fluorescent-tagged LC3. Red: RFP, green: GFP, white: TUJ1. Scale bar = 5 μm. (j) Quantification of the GFP-/RFP+ puncta. (n=5, mean ± SEM, unpaired Student’s t-test; * p =0.0164)
    Dna Construct Encoding Gfp Cortactin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) Representative images of day 6 neurons after Lysotracker treatment. Lysotracker is shown in magenta, and DAPI in blue. Scale bar = 20 μm. (b) Quantification of Lysotracker signal in day 6 neurons. The relative Lysotracker intensity of CLCN4 -variant neurons compared to the WT was presented. Each dot represents one biological replicate. (n=3-4, mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test * p =0.039 for variant A; *** p =0.0007 for variant B; ** p =0.0092 for variant C) The magenta dashed line indicates the value for WT. (c) Relative acidity in acidic subcellular organelles. Each dot represents one biological replicate. (n=3, mean ± SEM, unpaired Student’s t-test; p =0.2894) (d-g) Dynamics of endo-lysosomal vesicles. RAB5, RAB7, RAB11 and LAMP1 indicates early endosome, late endosome, recycling endosome and lysosome markers, respectively. (RAB5, n=6; RAB7, n=3; RAB11, n=4; and LAMP1, n=3 or 4; mean ± SEM, unpaired Student’s t-test) (d) Representative images of a neuron with GFP-labeled vesicular organelles. TUJ1 and DAPI are shown in red and blue, respectively. Scale bar = 10 μm. (e) Relative GFP intensity expressed in vesicular organelles (* p =0.0265 for RAB5, * p =0.0158 for RAB7, * p =0.0475 for RAB11, and ** p =0.0067 for LAMP1) (f) Number of GFP-expressing vesicles in neurons. (* p =0.0186 for RAB5, * *p =0.0066 for RAB7, and p =0.9031 for LAMP1) (g) Average distance of GFP-expressing vesicles from the center of nucleus, representing the mean linear distance of puncta from the center of the nucleus in each cell. (** p =0.0011 for RAB5, * p =0.0463 for RAB7, and * p =0.0103 for LAMP1) (h) Scheme of autophagic flux analysis using tandem fluorescent protein fused to LC3. (i) Representative images of day 6 neurons expressing tandem fluorescent-tagged LC3. Red: RFP, green: GFP, white: TUJ1. Scale bar = 5 μm. (j) Quantification of the GFP-/RFP+ puncta. (n=5, mean ± SEM, unpaired Student’s t-test; * p =0.0164)

    Journal: bioRxiv

    Article Title: MEG3 Enhances Survival of Developing Human Neurons with CLCN4 -Linked Autophagy Impairment

    doi: 10.1101/2025.07.16.665078

    Figure Lengend Snippet: (a) Representative images of day 6 neurons after Lysotracker treatment. Lysotracker is shown in magenta, and DAPI in blue. Scale bar = 20 μm. (b) Quantification of Lysotracker signal in day 6 neurons. The relative Lysotracker intensity of CLCN4 -variant neurons compared to the WT was presented. Each dot represents one biological replicate. (n=3-4, mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test * p =0.039 for variant A; *** p =0.0007 for variant B; ** p =0.0092 for variant C) The magenta dashed line indicates the value for WT. (c) Relative acidity in acidic subcellular organelles. Each dot represents one biological replicate. (n=3, mean ± SEM, unpaired Student’s t-test; p =0.2894) (d-g) Dynamics of endo-lysosomal vesicles. RAB5, RAB7, RAB11 and LAMP1 indicates early endosome, late endosome, recycling endosome and lysosome markers, respectively. (RAB5, n=6; RAB7, n=3; RAB11, n=4; and LAMP1, n=3 or 4; mean ± SEM, unpaired Student’s t-test) (d) Representative images of a neuron with GFP-labeled vesicular organelles. TUJ1 and DAPI are shown in red and blue, respectively. Scale bar = 10 μm. (e) Relative GFP intensity expressed in vesicular organelles (* p =0.0265 for RAB5, * p =0.0158 for RAB7, * p =0.0475 for RAB11, and ** p =0.0067 for LAMP1) (f) Number of GFP-expressing vesicles in neurons. (* p =0.0186 for RAB5, * *p =0.0066 for RAB7, and p =0.9031 for LAMP1) (g) Average distance of GFP-expressing vesicles from the center of nucleus, representing the mean linear distance of puncta from the center of the nucleus in each cell. (** p =0.0011 for RAB5, * p =0.0463 for RAB7, and * p =0.0103 for LAMP1) (h) Scheme of autophagic flux analysis using tandem fluorescent protein fused to LC3. (i) Representative images of day 6 neurons expressing tandem fluorescent-tagged LC3. Red: RFP, green: GFP, white: TUJ1. Scale bar = 5 μm. (j) Quantification of the GFP-/RFP+ puncta. (n=5, mean ± SEM, unpaired Student’s t-test; * p =0.0164)

    Article Snippet: For specific subcellular vesicle visualization, lentiviral constructs encoding GFP-tagged RAB5 (Addgene #134858), RAB7 (Addgene #133027), RAB11 (Addgene #134860), and LAMP1 (Addgene #134868) were utilized.

    Techniques: Variant Assay, Labeling, Expressing

    (a) Representative images of day 6 neurons after Lysotracker treatment. Lysotracker is shown in magenta, and DAPI in blue. Scale bar = 20 μm. (b) Quantification of Lysotracker signal in day 6 neurons. The relative Lysotracker intensity of CLCN4 -variant neurons compared to the WT was presented. Each dot represents one biological replicate. (n=3-4, mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test * p =0.039 for variant A; *** p =0.0007 for variant B; ** p =0.0092 for variant C) The magenta dashed line indicates the value for WT. (c) Relative acidity in acidic subcellular organelles. Each dot represents one biological replicate. (n=3, mean ± SEM, unpaired Student’s t-test; p =0.2894) (d-g) Dynamics of endo-lysosomal vesicles. RAB5, RAB7, RAB11 and LAMP1 indicates early endosome, late endosome, recycling endosome and lysosome markers, respectively. (RAB5, n=6; RAB7, n=3; RAB11, n=4; and LAMP1, n=3 or 4; mean ± SEM, unpaired Student’s t-test) (d) Representative images of a neuron with GFP-labeled vesicular organelles. TUJ1 and DAPI are shown in red and blue, respectively. Scale bar = 10 μm. (e) Relative GFP intensity expressed in vesicular organelles (* p =0.0265 for RAB5, * p =0.0158 for RAB7, * p =0.0475 for RAB11, and ** p =0.0067 for LAMP1) (f) Number of GFP-expressing vesicles in neurons. (* p =0.0186 for RAB5, * *p =0.0066 for RAB7, and p =0.9031 for LAMP1) (g) Average distance of GFP-expressing vesicles from the center of nucleus, representing the mean linear distance of puncta from the center of the nucleus in each cell. (** p =0.0011 for RAB5, * p =0.0463 for RAB7, and * p =0.0103 for LAMP1) (h) Scheme of autophagic flux analysis using tandem fluorescent protein fused to LC3. (i) Representative images of day 6 neurons expressing tandem fluorescent-tagged LC3. Red: RFP, green: GFP, white: TUJ1. Scale bar = 5 μm. (j) Quantification of the GFP-/RFP+ puncta. (n=5, mean ± SEM, unpaired Student’s t-test; * p =0.0164)

    Journal: bioRxiv

    Article Title: MEG3 Enhances Survival of Developing Human Neurons with CLCN4 -Linked Autophagy Impairment

    doi: 10.1101/2025.07.16.665078

    Figure Lengend Snippet: (a) Representative images of day 6 neurons after Lysotracker treatment. Lysotracker is shown in magenta, and DAPI in blue. Scale bar = 20 μm. (b) Quantification of Lysotracker signal in day 6 neurons. The relative Lysotracker intensity of CLCN4 -variant neurons compared to the WT was presented. Each dot represents one biological replicate. (n=3-4, mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test * p =0.039 for variant A; *** p =0.0007 for variant B; ** p =0.0092 for variant C) The magenta dashed line indicates the value for WT. (c) Relative acidity in acidic subcellular organelles. Each dot represents one biological replicate. (n=3, mean ± SEM, unpaired Student’s t-test; p =0.2894) (d-g) Dynamics of endo-lysosomal vesicles. RAB5, RAB7, RAB11 and LAMP1 indicates early endosome, late endosome, recycling endosome and lysosome markers, respectively. (RAB5, n=6; RAB7, n=3; RAB11, n=4; and LAMP1, n=3 or 4; mean ± SEM, unpaired Student’s t-test) (d) Representative images of a neuron with GFP-labeled vesicular organelles. TUJ1 and DAPI are shown in red and blue, respectively. Scale bar = 10 μm. (e) Relative GFP intensity expressed in vesicular organelles (* p =0.0265 for RAB5, * p =0.0158 for RAB7, * p =0.0475 for RAB11, and ** p =0.0067 for LAMP1) (f) Number of GFP-expressing vesicles in neurons. (* p =0.0186 for RAB5, * *p =0.0066 for RAB7, and p =0.9031 for LAMP1) (g) Average distance of GFP-expressing vesicles from the center of nucleus, representing the mean linear distance of puncta from the center of the nucleus in each cell. (** p =0.0011 for RAB5, * p =0.0463 for RAB7, and * p =0.0103 for LAMP1) (h) Scheme of autophagic flux analysis using tandem fluorescent protein fused to LC3. (i) Representative images of day 6 neurons expressing tandem fluorescent-tagged LC3. Red: RFP, green: GFP, white: TUJ1. Scale bar = 5 μm. (j) Quantification of the GFP-/RFP+ puncta. (n=5, mean ± SEM, unpaired Student’s t-test; * p =0.0164)

    Article Snippet: For specific subcellular vesicle visualization, lentiviral constructs encoding GFP-tagged RAB5 (Addgene #134858), RAB7 (Addgene #133027), RAB11 (Addgene #134860), and LAMP1 (Addgene #134868) were utilized.

    Techniques: Variant Assay, Labeling, Expressing