Cell Culture:Article Title: Preparation and Characterization of Alginate-Based Bioinks for Three-Dimensional Bioprinting of Cell-Laden Constructs.
Article Snippet: .. Materials HUVEC complete cell culture medium (see recipe) HUV-EC-C (HUVEC) (ATCC, cat. no. CRL-1730) Dahlan et al. 3 of 39 Current Protocols 26911299, 2025, 12, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70290 by N at Prov Indonesia, W iley O nline L ibrary on [01/01/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate-buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma-Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma-Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, 2006) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15-ml conical centrifuge tubes (Falcon, cat. no. 14-959-49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8-in. (Fisher Scientific, cat. no. 21-401-10) Magnetic stir bars Weighing papers 50-ml conical centrifuge tubes (Falcon, cat. no. 14-532-22) Scale Parafilm Magnetic stirring hot plate 20-, 200-, and 1000-μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma-Aldrich, cat. no. 928879) Culturing HUVECs for cell expansion Seeding cells 1.
Article Title: Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Article Snippet: .. HUVEC complete cell culture medium (see recipe) HUV‐EC‐C (HUVEC) (ATCC, cat. no. CRL‐1730) 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate‐buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma‐Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma‐Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, ) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15‐ml conical centrifuge tubes (Falcon, cat. no. 14‐959‐49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO 2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8‐in. (Fisher Scientific, cat. no. 21‐401‐10) Magnetic stir bars Weighing papers 50‐ml conical centrifuge tubes (Falcon, cat. no. 14‐532‐22) Scale Parafilm Magnetic stirring hot plate 20‐, 200‐, and 1000‐μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma‐Aldrich, cat. no. 928879) ..
Saline:Article Title: Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Article Snippet: .. HUVEC complete cell culture medium (see recipe) HUV‐EC‐C (HUVEC) (ATCC, cat. no. CRL‐1730) 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate‐buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma‐Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma‐Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, ) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15‐ml conical centrifuge tubes (Falcon, cat. no. 14‐959‐49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO 2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8‐in. (Fisher Scientific, cat. no. 21‐401‐10) Magnetic stir bars Weighing papers 50‐ml conical centrifuge tubes (Falcon, cat. no. 14‐532‐22) Scale Parafilm Magnetic stirring hot plate 20‐, 200‐, and 1000‐μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma‐Aldrich, cat. no. 928879) ..
Algae:Article Title: Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Article Snippet: .. HUVEC complete cell culture medium (see recipe) HUV‐EC‐C (HUVEC) (ATCC, cat. no. CRL‐1730) 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate‐buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma‐Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma‐Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, ) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15‐ml conical centrifuge tubes (Falcon, cat. no. 14‐959‐49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO 2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8‐in. (Fisher Scientific, cat. no. 21‐401‐10) Magnetic stir bars Weighing papers 50‐ml conical centrifuge tubes (Falcon, cat. no. 14‐532‐22) Scale Parafilm Magnetic stirring hot plate 20‐, 200‐, and 1000‐μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma‐Aldrich, cat. no. 928879) ..
Viscosity:Article Title: Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Article Snippet: .. HUVEC complete cell culture medium (see recipe) HUV‐EC‐C (HUVEC) (ATCC, cat. no. CRL‐1730) 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate‐buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma‐Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma‐Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, ) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15‐ml conical centrifuge tubes (Falcon, cat. no. 14‐959‐49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO 2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8‐in. (Fisher Scientific, cat. no. 21‐401‐10) Magnetic stir bars Weighing papers 50‐ml conical centrifuge tubes (Falcon, cat. no. 14‐532‐22) Scale Parafilm Magnetic stirring hot plate 20‐, 200‐, and 1000‐μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma‐Aldrich, cat. no. 928879) ..
Transferring:Article Title: Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Article Snippet: .. HUVEC complete cell culture medium (see recipe) HUV‐EC‐C (HUVEC) (ATCC, cat. no. CRL‐1730) 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate‐buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma‐Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma‐Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, ) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15‐ml conical centrifuge tubes (Falcon, cat. no. 14‐959‐49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO 2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8‐in. (Fisher Scientific, cat. no. 21‐401‐10) Magnetic stir bars Weighing papers 50‐ml conical centrifuge tubes (Falcon, cat. no. 14‐532‐22) Scale Parafilm Magnetic stirring hot plate 20‐, 200‐, and 1000‐μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma‐Aldrich, cat. no. 928879) ..
Microscopy:Article Title: Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Article Snippet: .. HUVEC complete cell culture medium (see recipe) HUV‐EC‐C (HUVEC) (ATCC, cat. no. CRL‐1730) 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate‐buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma‐Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma‐Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, ) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15‐ml conical centrifuge tubes (Falcon, cat. no. 14‐959‐49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO 2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8‐in. (Fisher Scientific, cat. no. 21‐401‐10) Magnetic stir bars Weighing papers 50‐ml conical centrifuge tubes (Falcon, cat. no. 14‐532‐22) Scale Parafilm Magnetic stirring hot plate 20‐, 200‐, and 1000‐μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma‐Aldrich, cat. no. 928879) ..
Sterility:Article Title: Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Article Snippet: .. HUVEC complete cell culture medium (see recipe) HUV‐EC‐C (HUVEC) (ATCC, cat. no. CRL‐1730) 70% ethanol Gibco TrypLE Express enzyme (1×), phenol red (Fisher Scientific, cat. no. 12605010); store at 4°C Gibco phosphate‐buffered saline (PBS), pH 7.4 (Fisher Scientific, cat. no. 10010023); store at room temperature Gibco trypan blue solution, 0.4% (Fisher Scientific, cat. no. 15250061); store at room temperature Alginic acid sodium salt from brown algae, medium viscosity (Sigma‐Aldrich, cat. no. A2033); store at 4°C Porcine gelatin, type A, gel strength ∼300 g bloom (Sigma‐Aldrich, cat. no. G1890); store at room temperature 0.9% NaCl solution (see Current Protocols, ) Biological safety cabinet (BSC) T75 culture flask (Corning, cat. no. 430641U) Serological pipettes Pipette controller 15‐ml conical centrifuge tubes (Falcon, cat. no. 14‐959‐49B) Water bath Centrifuge Cell culture microscope Cell culture incubator, 37°C, 5% CO 2 Hemocytometer or automated cell counter Autoclave Beakers Spatula, micro, tapered, 8‐in. (Fisher Scientific, cat. no. 21‐401‐10) Magnetic stir bars Weighing papers 50‐ml conical centrifuge tubes (Falcon, cat. no. 14‐532‐22) Scale Parafilm Magnetic stirring hot plate 20‐, 200‐, and 1000‐μl micropipettes and tips Sterile printing syringe for bioprinting or syringe barrels (Sigma‐Aldrich, cat. no. 928879) ..
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