reference strain e coli atcc 25922  (ATCC)

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: EC 50 results for Vibrio strains against GATR-3, LL-37-NH 2 , and Mastoparan-AF-NH 2 peptides. Vibrio vulnificus MO6 EC 50 results against A. GATR-3, B. LL-37-NH 2 , and C. Mastoparan-AF-NH 2 peptides. Vibrio vulnificus JY1701 EC 50 results against D. GATR-3, E. LL-37-NH 2 , and F. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus NY477 EC 50 results against G. GATR-3, H. LL-37-NH 2 , and I. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus SAK11 EC 50 results against J. GATR-3, K. LL-37-NH 2 , and L. Mastoparan-AF-NH 2 peptides. Escherichia coli EC 50 results against M. GATR-3, N. LL-37-NH 2 , and O. Mastoparan-AF-NH 2 peptides.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques:

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: Biofilm Formation of Vibrio isolates and E. coli . Quantitative comparison of biofilm formation among Vibrio isolates and E. coli after 24 h incubation. Biofilm biomass was determined by crystal violet staining and measurement of OD₆₀₀. V. vulnificus MO6 and V. parahaemolyticus NY477 exhibited the highest biofilm-forming capacities.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques: Comparison, Incubation, Staining

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: UMI-guided single locus sequence typing method for phylotyping Cutibacterium acnes from skin samples

doi: 10.3389/fcimb.2026.1807759

Figure Lengend Snippet: Performance of the SLST assay on spike-in mixtures of C. acnes DNA with increasing background DNA. (A) Total number of full-length SLST sequences reconstructed from spike-in mixtures containing decreasing proportions of C. acnes genomic DNA (SpikeMix-50, -10, -5, and -0.5) supplemented with E. coli DNA. Bars represent mean counts across replicates (± SD), and dots indicate individual replicates. (B) Relative abundances of SLST types recovered from the same spike-in mixtures, shown alongside the theoretical composition (Expected, left). Stacked bars illustrate the five targeted SLST types (A1, G1, H2, K2, L1), with residual reads grouped as “Others (< 1%)” and “Unassigned.” All SpikeMix samples were processed without pre-amplification, using 2 µL of purified DNA at 0.125 ng/µL (total input 0.25 ng) directly into the molecular barcoding step, yielding absolute C. acnes DNA inputs of 125 pg (SpikeMix-50), 25 pg (SpikeMix-10), 12.5 pg (SpikeMix-5), and 1.25 pg (SpikeMix-0.5).

Article Snippet: This community was supplemented with Escherichia coli genomic DNA (ATCC 8739D-5) to progressively reduce the fraction of C. acnes DNA.

Techniques: Amplification, Purification