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mouse rectal epithelial cell line cmt93  (ATCC)


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    ATCC mouse rectal epithelial cell line cmt93
    Mouse Rectal Epithelial Cell Line Cmt93, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmt+93/CMT-93/pm42043053-288-14-21
    Average 95 stars, based on 263 article reviews
    mouse rectal epithelial cell line cmt93 - by Bioz Stars, 2026-09
    95/100 stars

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    Article Title: Claudin-7 deficiency induces metabolic reprogramming of neutrophils in the colorectal cancer microenvironment
    Article Snippet: MC38, CMT-93, CT26, and HCT116 cells were obtained from the American Type Culture Collection (ATCC, USA).

    Article Title: Olfactomedin-4 + neutrophils exacerbate intestinal epithelial damage and worsen host survival after Clostridioides difficile infection
    Article Snippet: For TEER assays, sorted neutrophils were added to CMT-93 (mouse colonic epithelial cells; ATCC) monolayer on 96-well E-plate (Agilent # 300601010), and changes in TEER were measured every 15 minutes for up to 72hrs as described previously ( ) and in Supplementary methods.

    Article Title: Oxaliplatin Enhances the Apoptotic Effect of Mesenchymal Stem Cells, Delivering Soluble TRAIL in Chemoresistant Colorectal Cancer
    Article Snippet: The adenocarcinoma cell lines Caco-2 (ATCC® HTB-37) and CMT-93 (ATCC® CCL-223 TM ) were used for assays.

    Article Title: Claudin-7 deficiency induces metabolic reprogramming of neutrophils in the colorectal cancer microenvironment.
    Article Snippet: MC38, CMT-93, CT26, and HCT116 cells were obtained from the American Type Culture Collection (ATCC, USA).

    Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth
    Article Snippet: WEHI-231, A20, J558, L1210, 70z/3, BW5147.3, YAC-1, S194/5, MPC-11, P815, B16-F10, CMT-93, L929, 293 T, NCI-H209, SK-MEL-28, and Phoenix-ECO cell lines were purchased from American Type Culture Collection (Rockville, MA).

    Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth.
    Article Snippet: WEHI-231, A20, J558, L1210, 70z/3, BW5147.3, YAC-1, S194/5,MPC-11, P815, B16-F10, CMT-93, L929, 293T, NCI-H209, SK-MEL-28, and PhoenixECO cell lines were purchased from American Type Culture Collection (Rockville, MA).

    Article Title: Sialic acid plays a pivotal role in licensing Citrobacter rodentium's transition from the intestinal lumen to a mucosal adherent niche.
    Article Snippet: CMT- 93 (mouse rectal epithelial) cells (ATCC CCL- 223) were seeded in 24- well plates at a density of 5 × 104 cells/well and grown until reaching >90% confluence (37 °C, 5% CO2).

    Article Title: Oxaliplatin Enhances the Apoptotic Effect of Mesenchymal Stem Cells, Delivering Soluble TRAIL in Chemoresistant Colorectal Cancer.
    Article Snippet: The adenocarcinoma cell lines Caco-2 (ATCC®HTB-37) and CMT-93 (ATCC®CCL223TM) were used for assays.



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    Plasmid DNA delivery from LL to different cell types. ( A ) Luciferase activity in RAW264.7, THP-1, <t>CMT-93,</t> and ARPE-19 cells co-cultured with LL/pLEC-Nanoluc at the indicated cell-to-bacteria ratios for 20 h. Data are mean ± SEM ( n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant by one-way ANOVA with Dunnett’s test (vs ratio 0 for each cell line). ( B ) Fluorescent and phase-contrast images of cells co-cultured with CFSE-labeled LL for 4 h. ( C ) Schematic of time-course experiments. RAW264.7 cells were co-cultured with LL/pLEC-Nanoluc or CFSE-labeled LL; after 2 h, medium was replaced with gentamicin-containing medium, and luciferase activity was measured at the indicated times. ( D ) Time course of luciferase activity in RAW264.7 cells (mean ± SEM, n = 5/time point; the symbols hide some error bars). ( E ) Representative percentage of EGFP-expressing RAW264.7 cells after 20 h co-culture with LL/pLEC-EGFP (mean ± SEM, n = 4). *** P < 0.001 by Student’s t -test. ( F ) Luciferase activity in adhered splenocytes co-cultured with LL/pLEC-Nanoluc for 24 h (mean ± SEM, n = 5). ** P < 0.01 by Mann-Whitney U-test. Cell-to-bacteria ratio was 1:1,000 for panels B, D, and E and 1:10 for panel F.
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    Plasmid DNA delivery from LL to different cell types. ( A ) Luciferase activity in RAW264.7, THP-1, <t>CMT-93,</t> and ARPE-19 cells co-cultured with LL/pLEC-Nanoluc at the indicated cell-to-bacteria ratios for 20 h. Data are mean ± SEM ( n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant by one-way ANOVA with Dunnett’s test (vs ratio 0 for each cell line). ( B ) Fluorescent and phase-contrast images of cells co-cultured with CFSE-labeled LL for 4 h. ( C ) Schematic of time-course experiments. RAW264.7 cells were co-cultured with LL/pLEC-Nanoluc or CFSE-labeled LL; after 2 h, medium was replaced with gentamicin-containing medium, and luciferase activity was measured at the indicated times. ( D ) Time course of luciferase activity in RAW264.7 cells (mean ± SEM, n = 5/time point; the symbols hide some error bars). ( E ) Representative percentage of EGFP-expressing RAW264.7 cells after 20 h co-culture with LL/pLEC-EGFP (mean ± SEM, n = 4). *** P < 0.001 by Student’s t -test. ( F ) Luciferase activity in adhered splenocytes co-cultured with LL/pLEC-Nanoluc for 24 h (mean ± SEM, n = 5). ** P < 0.01 by Mann-Whitney U-test. Cell-to-bacteria ratio was 1:1,000 for panels B, D, and E and 1:10 for panel F.
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    Plasmid DNA delivery from LL to different cell types. ( A ) Luciferase activity in RAW264.7, THP-1, <t>CMT-93,</t> and ARPE-19 cells co-cultured with LL/pLEC-Nanoluc at the indicated cell-to-bacteria ratios for 20 h. Data are mean ± SEM ( n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant by one-way ANOVA with Dunnett’s test (vs ratio 0 for each cell line). ( B ) Fluorescent and phase-contrast images of cells co-cultured with CFSE-labeled LL for 4 h. ( C ) Schematic of time-course experiments. RAW264.7 cells were co-cultured with LL/pLEC-Nanoluc or CFSE-labeled LL; after 2 h, medium was replaced with gentamicin-containing medium, and luciferase activity was measured at the indicated times. ( D ) Time course of luciferase activity in RAW264.7 cells (mean ± SEM, n = 5/time point; the symbols hide some error bars). ( E ) Representative percentage of EGFP-expressing RAW264.7 cells after 20 h co-culture with LL/pLEC-EGFP (mean ± SEM, n = 4). *** P < 0.001 by Student’s t -test. ( F ) Luciferase activity in adhered splenocytes co-cultured with LL/pLEC-Nanoluc for 24 h (mean ± SEM, n = 5). ** P < 0.01 by Mann-Whitney U-test. Cell-to-bacteria ratio was 1:1,000 for panels B, D, and E and 1:10 for panel F.
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    Plasmid DNA delivery from LL to different cell types. ( A ) Luciferase activity in RAW264.7, THP-1, <t>CMT-93,</t> and ARPE-19 cells co-cultured with LL/pLEC-Nanoluc at the indicated cell-to-bacteria ratios for 20 h. Data are mean ± SEM ( n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant by one-way ANOVA with Dunnett’s test (vs ratio 0 for each cell line). ( B ) Fluorescent and phase-contrast images of cells co-cultured with CFSE-labeled LL for 4 h. ( C ) Schematic of time-course experiments. RAW264.7 cells were co-cultured with LL/pLEC-Nanoluc or CFSE-labeled LL; after 2 h, medium was replaced with gentamicin-containing medium, and luciferase activity was measured at the indicated times. ( D ) Time course of luciferase activity in RAW264.7 cells (mean ± SEM, n = 5/time point; the symbols hide some error bars). ( E ) Representative percentage of EGFP-expressing RAW264.7 cells after 20 h co-culture with LL/pLEC-EGFP (mean ± SEM, n = 4). *** P < 0.001 by Student’s t -test. ( F ) Luciferase activity in adhered splenocytes co-cultured with LL/pLEC-Nanoluc for 24 h (mean ± SEM, n = 5). ** P < 0.01 by Mann-Whitney U-test. Cell-to-bacteria ratio was 1:1,000 for panels B, D, and E and 1:10 for panel F.
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    Plasmid DNA delivery from LL to different cell types. ( A ) Luciferase activity in RAW264.7, THP-1, CMT-93, and ARPE-19 cells co-cultured with LL/pLEC-Nanoluc at the indicated cell-to-bacteria ratios for 20 h. Data are mean ± SEM ( n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant by one-way ANOVA with Dunnett’s test (vs ratio 0 for each cell line). ( B ) Fluorescent and phase-contrast images of cells co-cultured with CFSE-labeled LL for 4 h. ( C ) Schematic of time-course experiments. RAW264.7 cells were co-cultured with LL/pLEC-Nanoluc or CFSE-labeled LL; after 2 h, medium was replaced with gentamicin-containing medium, and luciferase activity was measured at the indicated times. ( D ) Time course of luciferase activity in RAW264.7 cells (mean ± SEM, n = 5/time point; the symbols hide some error bars). ( E ) Representative percentage of EGFP-expressing RAW264.7 cells after 20 h co-culture with LL/pLEC-EGFP (mean ± SEM, n = 4). *** P < 0.001 by Student’s t -test. ( F ) Luciferase activity in adhered splenocytes co-cultured with LL/pLEC-Nanoluc for 24 h (mean ± SEM, n = 5). ** P < 0.01 by Mann-Whitney U-test. Cell-to-bacteria ratio was 1:1,000 for panels B, D, and E and 1:10 for panel F.

    Journal: Infection and Immunity

    Article Title: Immunogenicity and plasmid delivery pathways of non-invasive Lactococcus lactis -vectored mucosal DNA vaccination

    doi: 10.1128/iai.00460-25

    Figure Lengend Snippet: Plasmid DNA delivery from LL to different cell types. ( A ) Luciferase activity in RAW264.7, THP-1, CMT-93, and ARPE-19 cells co-cultured with LL/pLEC-Nanoluc at the indicated cell-to-bacteria ratios for 20 h. Data are mean ± SEM ( n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant by one-way ANOVA with Dunnett’s test (vs ratio 0 for each cell line). ( B ) Fluorescent and phase-contrast images of cells co-cultured with CFSE-labeled LL for 4 h. ( C ) Schematic of time-course experiments. RAW264.7 cells were co-cultured with LL/pLEC-Nanoluc or CFSE-labeled LL; after 2 h, medium was replaced with gentamicin-containing medium, and luciferase activity was measured at the indicated times. ( D ) Time course of luciferase activity in RAW264.7 cells (mean ± SEM, n = 5/time point; the symbols hide some error bars). ( E ) Representative percentage of EGFP-expressing RAW264.7 cells after 20 h co-culture with LL/pLEC-EGFP (mean ± SEM, n = 4). *** P < 0.001 by Student’s t -test. ( F ) Luciferase activity in adhered splenocytes co-cultured with LL/pLEC-Nanoluc for 24 h (mean ± SEM, n = 5). ** P < 0.01 by Mann-Whitney U-test. Cell-to-bacteria ratio was 1:1,000 for panels B, D, and E and 1:10 for panel F.

    Article Snippet: The murine colonic epithelial cell line CMT-93 (ATCC) was cultured in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS at 37°C in 5% CO 2 .

    Techniques: Plasmid Preparation, Luciferase, Activity Assay, Cell Culture, Bacteria, Labeling, Expressing, Co-Culture Assay, MANN-WHITNEY