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cmf buffer  (Thermo Fisher)


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    Thermo Fisher cmf buffer
    Cmf Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmf+buffer/Hanks'+Balanced+Salt+Solution/10__1038_slash_s41590___026___02480___z-256-8-16
    Average 99 stars, based on 1 article reviews
    cmf buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: MHC class I on target cells regulates CD4+ T cell-mediated immunity
    Article Snippet: Primary IECs were obtained from C57BL/6 mice as described previously1.Primary IECs were obtained from C57BL/6 mice as described previously1.. Briefly, luminal contents of intestine were flushed with CMF buffer (Ca2+/Mg2+-free Hanks’ balanced salt solution (14185052, Thermo Fisher Scientific) supplemented with 25 mM sodium bicarbonate (S6014, Sigma-Aldrich) and 2% FBS (100-106, Gemini Bio Products)).. The intestine was cut longitudinally, then minced into 50-mm pieces, washed with CMF six times, transferred to CMF with 5 mM EDTA (51201, Lonza) and incubated at 37 °C for 45 min (shaking tubes every 15 min).The intestine was cut longitudinally, then minced into 50-mm pieces, washed with CMF six times, transferred to CMF with 5 mM EDTA (51201, Lonza) and incubated at 37 °C for 45 min (shaking tubes every 15 min).

    Article Title: Autophagy differentially regulates tissue tolerance of distinct target organs in graft-versus-host disease models
    Article Snippet: Luminal contents from dissected small and large intestines were flushed with CMF buffer (Ca 2+ /Mg 2+ -Free Hanks balanced salt solution, Thermo Fisher Scientific), supplemented with 25 mM sodium bicarbonate (Sigma-Aldrich) and 2% FBS (Gemini Bio Products). .. I



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Isolation of murine hepatic myeloid cells with high yield and purity using immunomagnetic beads for subset analysis

    doi: 10.1016/j.xpro.2023.102592

    Figure Lengend Snippet:

    Article Snippet: CMF-HBSS buffer (without Ca 2+ and Mg 2+ ) , Gibco , Cat# 14175-095.

    Techniques: Recombinant, Plasmid Preparation, Blocking Assay, Purification, Staining, Hood, Software

    Perfusion buffer (PB)

    Journal: STAR Protocols

    Article Title: Isolation of murine hepatic myeloid cells with high yield and purity using immunomagnetic beads for subset analysis

    doi: 10.1016/j.xpro.2023.102592

    Figure Lengend Snippet: Perfusion buffer (PB)

    Article Snippet: CMF-HBSS buffer (without Ca 2+ and Mg 2+ ) , Gibco , Cat# 14175-095.

    Techniques: Concentration Assay