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calcium- magnesium-free (cmf) buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher calcium- magnesium-free (cmf) buffer
    Calcium Magnesium Free (Cmf) Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmf+buffer/pmc10696420-56-29-25
    Average 90 stars, based on 1 article reviews
    calcium- magnesium-free (cmf) buffer - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Injection:

    Article Title: Molecular profiling of bladder cancer xenografts defines relevant molecular subtypes and provides a resource for biomarker discovery.
    Article Snippet: Bladder cancer (BLCA) genomic profiling has identified molecular subtypes with distinct clinical characteristics and variable sensitivities to frontline therapy.. BLCAs can be categorized into luminal or basal subtypes based on their gene expression.. We comprehensively characterized nine human BLCA cell lines (UC3, UC6, UC9, UC13, UC14, T24, SCaBER, RT4V6 and RT112) into molecular subtypes using orthotopic xenograft models.

    Concentration Assay:

    Article Title: Molecular profiling of bladder cancer xenografts defines relevant molecular subtypes and provides a resource for biomarker discovery.
    Article Snippet: Bladder cancer (BLCA) genomic profiling has identified molecular subtypes with distinct clinical characteristics and variable sensitivities to frontline therapy.. BLCAs can be categorized into luminal or basal subtypes based on their gene expression.. We comprehensively characterized nine human BLCA cell lines (UC3, UC6, UC9, UC13, UC14, T24, SCaBER, RT4V6 and RT112) into molecular subtypes using orthotopic xenograft models.

    other:

    Article Title: AAV capsid prioritization in normal and steatotic human livers maintained by machine perfusion.
    Article Snippet: Sections were then perfused with 1 mg ml–1 collagenase IV (Worthington) in HBSS containing 20 mM HEPES, penicillin–streptomycin and 5 mM CaCl2·H2O (Thermo Fisher Scientific) for 22 min at 37 °C.

    Extraction:

    Article Title: Ag-driven CD8 + T cell clonal expansion is a prominent feature of MASH in humans and mice
    Article Snippet: .. Briefly, livers were perfused with 10 mL HBSS (Gibco, Waltham, MA) before extraction and digestion with Collagenase Type IV (Worthington-Biochemical, Lakewood, NJ) and DNASE I (Sigma-Aldrich, Burlington, MA). .. NPCs were isolated using a 40% OptiPrep density gradient (Sigma-Aldrich), filtered, and washed.

    Construct:

    Article Title: In vitro cytotoxicity (irritant potency) of toothpaste ingredients
    Article Snippet: .. The lamina propria hydrogel compartment was constructed by mixing rat-tail collagen with 1:1 fibrinogen (Diagnostica Stago S.A.S., Asnieres sur Seine, France), Hank’s Balanced Salt Solution (HBSS; Gibco, Grand Island, USA) (HBSS was diluted 8-fold) and fibroblasts (3.25 × 10 4 cells/gel) [ ]. (Each hydrogel contained 0.5 U/ml thrombin (Merck KGaA, Darmstadt, Germany) to allow for fibrin formation. .. The fibroblast-populated hydrogels were cultured in a twelve-well transwell insert with 0.4 μm pores (ThinCertsTM, Greiner Bio/One, Alphen aan den Rijn, Netherlands) and incubated overnight submerged in fibroblast medium (DMEM, 5% Fetal Clone III (GE Healthcare, Chicago, USA), 1% penicillin-streptomycin (Gibco)).



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Isolation of murine hepatic myeloid cells with high yield and purity using immunomagnetic beads for subset analysis

    doi: 10.1016/j.xpro.2023.102592

    Figure Lengend Snippet:

    Article Snippet: CMF-HBSS buffer (without Ca 2+ and Mg 2+ ) , Gibco , Cat# 14175-095.

    Techniques: Recombinant, Plasmid Preparation, Blocking Assay, Purification, Staining, Hood, Software

    Perfusion buffer (PB)

    Journal: STAR Protocols

    Article Title: Isolation of murine hepatic myeloid cells with high yield and purity using immunomagnetic beads for subset analysis

    doi: 10.1016/j.xpro.2023.102592

    Figure Lengend Snippet: Perfusion buffer (PB)

    Article Snippet: CMF-HBSS buffer (without Ca 2+ and Mg 2+ ) , Gibco , Cat# 14175-095.

    Techniques: Concentration Assay