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Biacore biacore cm4 sensor chip
Biacore Cm4 Sensor Chip, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cm4+chip/chip+cm4+sensor/us12543711-2150-1-1
Average 86 stars, based on 1 article reviews
biacore cm4 sensor chip - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

SPR Assay:

Article Title: A dual-targeting PDGFR?/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo
Article Snippet: .. Surface plasmon resonance analysis (Biacore t100) of bsAb for simultaneous (dual) binding to VeGF-A and PDGFRβ. the bsAb was captured onto a CM4 chip via hVeGF-A. either 0 nM (blue) or 500 nM (red) pDGFRβ-Fc was then allowed to bind to the bsAb. table ft1 table-wrap mode="anchored" t5 caption a7 Binding to PDGFRβ Binding to VEGF-A K a (1/Ms) K d (1/s) K D (M) K a (1/Ms) k d (1/s) K D (M) Anti-PDGFRβ scFv 5 E+05 4 E-05 9 E-11 – – – Anti-VEGFA scFv – – – 3 E+05 2 E-04 6 E-10 Bispecific scFv-Fc-scFv 5 E+04 4 E-05 8 E-10 2 E+05 2 E-04 1 E-09 Open in a separate window Kinetics of binding to PDGFRβ and VEGF-A Inhibition of biological activity mediated by VEGF and PDGF in vitro. ..

Article Title: Short Communication: Inhibition of DC-SIGN-Mediated HIV-1 Infection by Complementary Actions of Dendritic Cell Receptor Antagonists and Env-Targeting Virus Inactivators
Article Snippet: .. SPR competition assays were performed on a Biacore 3000 using a CM4 chip, functionalized at 5 μl/min. ..

Binding Assay:

Article Title: A dual-targeting PDGFR?/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo
Article Snippet: .. Surface plasmon resonance analysis (Biacore t100) of bsAb for simultaneous (dual) binding to VeGF-A and PDGFRβ. the bsAb was captured onto a CM4 chip via hVeGF-A. either 0 nM (blue) or 500 nM (red) pDGFRβ-Fc was then allowed to bind to the bsAb. table ft1 table-wrap mode="anchored" t5 caption a7 Binding to PDGFRβ Binding to VEGF-A K a (1/Ms) K d (1/s) K D (M) K a (1/Ms) k d (1/s) K D (M) Anti-PDGFRβ scFv 5 E+05 4 E-05 9 E-11 – – – Anti-VEGFA scFv – – – 3 E+05 2 E-04 6 E-10 Bispecific scFv-Fc-scFv 5 E+04 4 E-05 8 E-10 2 E+05 2 E-04 1 E-09 Open in a separate window Kinetics of binding to PDGFRβ and VEGF-A Inhibition of biological activity mediated by VEGF and PDGF in vitro. ..

Article Title: Humanised anti kallikrein-2 antibody
Article Snippet: .. All binding experiments were performed on CM4 chip on a Biacore 3000 instrument. .. The chip and all the reagents needed for activation, immobilization, deactivation, binding and regeneration were purchased from GE Healthcare and used according to the guidelines from the manufacturer.

Article Title: Humanised anti kallikrein-2 antibody
Article Snippet: Reagents and Instrument Information Following solutions of the four antibodies and one antigen were provided by Diaprost AB: m11B6 stock: a-ehk211B6 14.12013 PP, 3.41 mg/ml: 0.9% NaCl, 100 μl h11B6 stock: Innovagen Lot 90476.30 2013-04-12, 1 mg/ml: PBS pH 7.4, 320 μl h11B6-DTPA stock: 0.2M Na-acetate pH 5.5, 0.9 mg/ml, 340 μl h11B6-DFO stock: 5 mg/ml gentisin acid in 0.2M ammonium acetate pH 5.5, 1.6 mg/ml, 400 μl hK2 stock: 26.6 μg/ml frakt 2 fr 7 SL+protein inh 5/2-02 1% BSA All the samples were aliquoted and kept in −20° C. freezer prior to analysis. .. All binding experiments were performed on CM4 chip on a Biacore 3000 instrument. .. The chip and all the reagents needed for activation, immobilization, deactivation, binding and regeneration were purchased from GE Healthcare and used according to the guidelines from the manufacturer.

Article Title: Binding, Neutralization and Internalization of the Interleukin-13 Antibody, Lebrikizumab
Article Snippet: .. The mAbs were captured on the surface of a CM4 chip with immobilized Protein A followed by the injection of different concentrations of IL-13 to assess the binding kinetics on a T200 Biacore. ..

Article Title: Interleukin-2 variant proteins fused to human IgG4 Fc and uses thereof
Article Snippet: Methods Fc-IL-2 variant binding data was collected on a Biacore platform using a CM4 chip. .. Methods Fc-IL-2 variant binding data was collected on a Biacore platform using a CM4 chip. ..

Article Title: PRLR-specific antibody and uses thereof
Article Snippet: .. TABLE 9 Sample KD kd ka Chi2 chXHA.06.275 Human 3.9E−10 2.7E−04 7.0E+05 0.574 he.06.275-1 Human 5.0E−10 3.1E−04 6.2E+05 0.594 he.06.275-2 Human 6.1E−10 3.7E−04 6.0E+05 0.532 he.06.275-3 Human 4.3E−10 2.7E−04 6.4E+05 0.555 he.06.275-4 Human 5.2E−10 3.2E−04 6.2E+05 0.69 chXHA.06.275 Cyno 1.2E−09 5.0E−04 4.3E+05 1.48 he.06.275-1 Cyno 1.6E−09 5.7E−04 3.6E+05 2.78 he.06.275-2 Cyno 1.9E−09 6.9E−04 3.7E+05 1.49 he.06.275-3 Cyno 1.3E−09 5.0E−04 3.9E+05 1.19 he.06.275-4 Cyno 1.7E−09 6.0E−04 3.6E+05 1.2 chXHA.06.642 Human 1.3E−09 4.6E−04 3.5E+05 5.15 he.06.642-1 Human 2.0E−09 3.5E−04 1.7E+05 12.9 he.06.642-2 Human 3.1E−09 5.3E−04 1.7E+05 12.6 chXHA.06.642 Cyno 26E−08 4.3E−03 1.7E+05 12.6 he.06.642-1 Cyno 3.1E−08 3.8E−03 1.2E+05 8.29 he.06.642-2 Cyno 4.7E−08 8.3E−03 1.8E+05 11.1 TABLE 10 Sample ka kd KD Res SD he.06.642 3 -G1 lot1 1.038(1)e5 3.828(5)e−4 3.688(5) nM 0.977 he.06.642 3 -G1 lot2 1.02E+5 3.88E−4 3.79977 nM 1.06 he.06.642 3 -G2 9.801(1)e4 4.210(7)e−4 4.296(6) nM 1.054 chXHA.06.642 1.962(3)e5 6.47E−04 3.296(5) nM 1.389 CHO.KLHG2-60 No binding No binding No binding No binding In order to compare rat and mouse cross reactivity using covalently immobilized antibodies, a CM4 chip was coupled with he.06.642-2 and he.06.275-4 antibodies via standard EDC-NHS amine coupling chemistries according to the recommended protocol from BIACORE® Inc. ..

Inhibition:

Article Title: A dual-targeting PDGFR?/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo
Article Snippet: .. Surface plasmon resonance analysis (Biacore t100) of bsAb for simultaneous (dual) binding to VeGF-A and PDGFRβ. the bsAb was captured onto a CM4 chip via hVeGF-A. either 0 nM (blue) or 500 nM (red) pDGFRβ-Fc was then allowed to bind to the bsAb. table ft1 table-wrap mode="anchored" t5 caption a7 Binding to PDGFRβ Binding to VEGF-A K a (1/Ms) K d (1/s) K D (M) K a (1/Ms) k d (1/s) K D (M) Anti-PDGFRβ scFv 5 E+05 4 E-05 9 E-11 – – – Anti-VEGFA scFv – – – 3 E+05 2 E-04 6 E-10 Bispecific scFv-Fc-scFv 5 E+04 4 E-05 8 E-10 2 E+05 2 E-04 1 E-09 Open in a separate window Kinetics of binding to PDGFRβ and VEGF-A Inhibition of biological activity mediated by VEGF and PDGF in vitro. ..

Activity Assay:

Article Title: A dual-targeting PDGFR?/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo
Article Snippet: .. Surface plasmon resonance analysis (Biacore t100) of bsAb for simultaneous (dual) binding to VeGF-A and PDGFRβ. the bsAb was captured onto a CM4 chip via hVeGF-A. either 0 nM (blue) or 500 nM (red) pDGFRβ-Fc was then allowed to bind to the bsAb. table ft1 table-wrap mode="anchored" t5 caption a7 Binding to PDGFRβ Binding to VEGF-A K a (1/Ms) K d (1/s) K D (M) K a (1/Ms) k d (1/s) K D (M) Anti-PDGFRβ scFv 5 E+05 4 E-05 9 E-11 – – – Anti-VEGFA scFv – – – 3 E+05 2 E-04 6 E-10 Bispecific scFv-Fc-scFv 5 E+04 4 E-05 8 E-10 2 E+05 2 E-04 1 E-09 Open in a separate window Kinetics of binding to PDGFRβ and VEGF-A Inhibition of biological activity mediated by VEGF and PDGF in vitro. ..

In Vitro:

Article Title: A dual-targeting PDGFR?/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo
Article Snippet: .. Surface plasmon resonance analysis (Biacore t100) of bsAb for simultaneous (dual) binding to VeGF-A and PDGFRβ. the bsAb was captured onto a CM4 chip via hVeGF-A. either 0 nM (blue) or 500 nM (red) pDGFRβ-Fc was then allowed to bind to the bsAb. table ft1 table-wrap mode="anchored" t5 caption a7 Binding to PDGFRβ Binding to VEGF-A K a (1/Ms) K d (1/s) K D (M) K a (1/Ms) k d (1/s) K D (M) Anti-PDGFRβ scFv 5 E+05 4 E-05 9 E-11 – – – Anti-VEGFA scFv – – – 3 E+05 2 E-04 6 E-10 Bispecific scFv-Fc-scFv 5 E+04 4 E-05 8 E-10 2 E+05 2 E-04 1 E-09 Open in a separate window Kinetics of binding to PDGFRβ and VEGF-A Inhibition of biological activity mediated by VEGF and PDGF in vitro. ..

other:

Article Title: HSA-related compositions and methods of use
Article Snippet: Both non-delipidated and delipidated forms of domain III were covalently immobilized at coupling densities 1016 and 1184 RUs respectively on CM4 (catalog no. BR-1005-39) or CM5 chip (catalog no. BR-1000-14) by standard amine coupling (BIAcore Handbook, 2002).

Injection:

Article Title: Binding, Neutralization and Internalization of the Interleukin-13 Antibody, Lebrikizumab
Article Snippet: .. The mAbs were captured on the surface of a CM4 chip with immobilized Protein A followed by the injection of different concentrations of IL-13 to assess the binding kinetics on a T200 Biacore. ..

Variant Assay:

Article Title: Interleukin-2 variant proteins fused to human IgG4 Fc and uses thereof
Article Snippet: Methods Fc-IL-2 variant binding data was collected on a Biacore platform using a CM4 chip. .. Methods Fc-IL-2 variant binding data was collected on a Biacore platform using a CM4 chip. ..



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