Journal: Frontiers in Veterinary Science

Article Title: Evaluation of clopidogrel impact on canine platelet function using flow cytometry and thromboelastography platelet mapping

doi: 10.3389/fvets.2025.1555641

Figure Lengend Snippet: Representative platelet mapping TEG tracing result. TEG platelet mapping tracings from an individual in the clopidogrel group are provided, with results shown before and after drug administration. Each tracing was obtained using citrated TEG analysis. The TEG software calculates the contribution of ADP-induced platelet activation to maximal amplitude using the following formula: ([MA ADP – MA fibrin /MA thrombin – MA fibrin ] × 100) and subtracts this value from 100 to derive percentage platelet inhibition. TEG, thromboelastography; MA, maximum amplitude.

Article Snippet: Dogs in the clopidogrel group received 1 mg/kg p.o., q24h of 75-mg clopidogrel (Plavix Tablet; Alvogen Korea Co., Ltd., South Korea), which was ground into a powder, and the dose was calculated for each dog.

Techniques: Software, Activation Assay, Inhibition

Journal: Frontiers in Veterinary Science

Article Title: Evaluation of clopidogrel impact on canine platelet function using flow cytometry and thromboelastography platelet mapping

doi: 10.3389/fvets.2025.1555641

Figure Lengend Snippet: The mean of the platelet mapping TEG parameter within groups over time. This figure shows the mean values of platelet mapping TEG parameters in the clopidogrel (solid line) and placebo (dashed line) groups on Days 0 and 7. Parameters measured include inhibition (%) and aggregation (%) to assess platelet inhibition and aggregation, respectively, along with MA values attributed to thrombin, fibrin, and ADP (MA_thrombin, MA_fibrin, and MA_ADP), which reflect platelet function in response to different activation pathways. * p < 0.05, indicating a significant difference between results for days 0 and 7 within each group. TEG, thromboelastography; MA, maximum amplitude; ADP, adenosine diphosphate.

Article Snippet: Dogs in the clopidogrel group received 1 mg/kg p.o., q24h of 75-mg clopidogrel (Plavix Tablet; Alvogen Korea Co., Ltd., South Korea), which was ground into a powder, and the dose was calculated for each dog.

Techniques: Inhibition, Activation Assay

Journal: Frontiers in Veterinary Science

Article Title: Evaluation of clopidogrel impact on canine platelet function using flow cytometry and thromboelastography platelet mapping

doi: 10.3389/fvets.2025.1555641

Figure Lengend Snippet: Results of the mean percentage of CD62P-positive cells and mean fluorescence intensity (MFI) in flow cytometry within groups over time. This figure shows the mean percentage of CD62P-positive cells in flow cytometry analysis for (A) the clopidogrel and (B) placebo groups on days 0 and 7. It also shows the results of the MFI in flow cytometry for (C) the clopidogrel group and (D) placebo group over time. Various conditions are shown, including unstimulated samples and samples stimulated with 20 μM ADP, 20 μM ADP + 20 μM epinephrine, 20 μM ADP + 12.5 μg/mL collagen, and 20 μM epinephrine. * p < 0.05 and ** p < 0.01, indicating a significant difference between results for days 0 and 7 within each group. ADP, adenosine diphosphate.

Article Snippet: Dogs in the clopidogrel group received 1 mg/kg p.o., q24h of 75-mg clopidogrel (Plavix Tablet; Alvogen Korea Co., Ltd., South Korea), which was ground into a powder, and the dose was calculated for each dog.

Techniques: Fluorescence, Flow Cytometry

Journal: Frontiers in Veterinary Science

Article Title: Evaluation of clopidogrel impact on canine platelet function using flow cytometry and thromboelastography platelet mapping

doi: 10.3389/fvets.2025.1555641

Figure Lengend Snippet: Individual results of platelet mapping TEG and flow cytometry in the clopidogrel group. This figure shows platelet mapping TEG and flow cytometry parameters for each dog in the clopidogrel group on days 0 and 7. Each line represents a different individual (identified by different colors and symbols). The parameters include MFI for ADP + epinephrine stimulation, aggregation (%), inhibition (%), and the percentage of CD62P-positive cells in flow cytometry. Despite baseline differences, all dogs exhibited a marked and consistent reduction in platelet function following clopidogrel treatment. ADP, adenosine diphosphate; MFI, mean fluorescence intensity; TEG, thromboelastography.

Article Snippet: Dogs in the clopidogrel group received 1 mg/kg p.o., q24h of 75-mg clopidogrel (Plavix Tablet; Alvogen Korea Co., Ltd., South Korea), which was ground into a powder, and the dose was calculated for each dog.

Techniques: Flow Cytometry, Inhibition, Fluorescence