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rabbit anti clc2  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti clc2
    Rabbit Anti Clc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clc/Anti-CLC-2+(CLCN2)+Antibody/pm42230621-288-26-28
    Average 92 stars, based on 14 article reviews
    rabbit anti clc2 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Staining:

    Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels
    Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 h, and then incubated for 48–72 h with CLC-3 primary antibody (Rabbit, 1:500, Alomone Labs, ACL-001). ..

    Article Title: Gluconate-based compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels.
    Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1M PBS) for 2 h, and then incubated for 48–72 h with CLC-3 primary antibody (Rabbit, 1:500, Alomone Labs, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Blocking Assay:

    Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels
    Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 h, and then incubated for 48–72 h with CLC-3 primary antibody (Rabbit, 1:500, Alomone Labs, ACL-001). ..

    Article Title: Gluconate-based compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels.
    Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1M PBS) for 2 h, and then incubated for 48–72 h with CLC-3 primary antibody (Rabbit, 1:500, Alomone Labs, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Incubation:

    Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels
    Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 h, and then incubated for 48–72 h with CLC-3 primary antibody (Rabbit, 1:500, Alomone Labs, ACL-001). ..

    Article Title: Gluconate-based compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells.
    Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex.

    Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels.
    Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1M PBS) for 2 h, and then incubated for 48–72 h with CLC-3 primary antibody (Rabbit, 1:500, Alomone Labs, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Severe changes in colon epithelium in the Mecp2 -null mouse model of Rett syndrome
    Article Snippet: .. Briefly, 4 mM paraffin sections were incubated with the corresponding primary antibody: ClC-2 1:400 (ACL-002, Alomone), NHE-3 1:1000 (C-20, Santa Cruz), and NKCC1 1:4000 (kindly provided by R. James Turner, [ ]). .. To detect bound antibodies, we used the LSAB Universal Kit (Dako).

    Immunostaining:

    Article Title: Gluconate-based compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Glucose oxidase compositions as a neonate anticonvulsant
    Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with CLC-3 primary antibody (Rabbit, 1:200, Alomone, ACL-001). ..

    Article Title: Poster Session Abstracts
    Article Snippet: .. The expression and tissue distribution of ClC-2 in mouse intestine was monitored by immunostaining using a C-terminal CLC-2 specific antibody (ACL-002, Alomone Labs). ..

    Purification:

    Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells.
    Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex.

    Centrifugation:

    Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells.
    Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex.

    Western Blot:

    Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells.
    Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex.

    SDS Page:

    Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells.
    Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex.

    Expressing:

    Article Title: Poster Session Abstracts
    Article Snippet: .. The expression and tissue distribution of ClC-2 in mouse intestine was monitored by immunostaining using a C-terminal CLC-2 specific antibody (ACL-002, Alomone Labs). ..



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    Image Search Results


    The in vivo therapeutic effect of Mn-NC composite hydrogels on TMJ-OA after 2 weeks. (a) Schematic diagram of in vivo experiments of hydrogel treatment of TMJ-OA. HE staining (b), SO/FG staining (c), immunohistochemical staining of COLII (d) and MMP13 (e), immunofluorescence staining of CD86 (f) and CD206 (g) at the synovial, and immunofluorescence staining of IL-1β (h) in the TMJ of rats after 2 weeks of treatment, scale bar = 100 μm. (i) Histological OARSI score of articular cartilage after 2 weeks of treatment and quantitative data of immunohistochemical positive area and proportion of immunofluorescence positive cells in (c–h). Data represent means ± SD, n = 3. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 indicates a significant difference in comparison to the Sham group. # p < 0.05, ## p < 0.01, and ### p < 0.001 suggests a significant difference in comparison to the MIA group.

    Journal: Bioactive Materials

    Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway

    doi: 10.1016/j.bioactmat.2026.01.031

    Figure Lengend Snippet: The in vivo therapeutic effect of Mn-NC composite hydrogels on TMJ-OA after 2 weeks. (a) Schematic diagram of in vivo experiments of hydrogel treatment of TMJ-OA. HE staining (b), SO/FG staining (c), immunohistochemical staining of COLII (d) and MMP13 (e), immunofluorescence staining of CD86 (f) and CD206 (g) at the synovial, and immunofluorescence staining of IL-1β (h) in the TMJ of rats after 2 weeks of treatment, scale bar = 100 μm. (i) Histological OARSI score of articular cartilage after 2 weeks of treatment and quantitative data of immunohistochemical positive area and proportion of immunofluorescence positive cells in (c–h). Data represent means ± SD, n = 3. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 indicates a significant difference in comparison to the Sham group. # p < 0.05, ## p < 0.01, and ### p < 0.001 suggests a significant difference in comparison to the MIA group.

    Article Snippet: Additionally, the sections were stained with antibodies specific to anti-CD86, CD206, and IL-1β (Boster Biological Technology Co., Ltd., Wuhan, China) for immunofluorescence analysis.

    Techniques: In Vivo, Staining, Immunohistochemical staining, Immunofluorescence, Comparison

    The in vivo therapeutic effect of Mn-NC composite hydrogels on TMJ-OA after 8 weeks. HE staining (a), SO/FG staining (b), immunohistochemical staining of COLII (c) and MMP13 (d), immunofluorescence staining of CD86 (e) and CD206 (f) at the synovial, and immunofluorescence staining of IL-1β (g) in the TMJ of rats after 8 weeks of treatment, scale bar = 100 μm. (h) Histological OARSI score of articular cartilage after 8 weeks of treatment and quantitative data of immunohistochemical positive area and proportion of immunofluorescence positive cells in (b–g). Data represent means ± SD, n = 3. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 indicates a significant difference in comparison to the Sham group. # p < 0.05, ## p < 0.01, and ### p < 0.001 suggests a significant difference in comparison to the MIA group.

    Journal: Bioactive Materials

    Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway

    doi: 10.1016/j.bioactmat.2026.01.031

    Figure Lengend Snippet: The in vivo therapeutic effect of Mn-NC composite hydrogels on TMJ-OA after 8 weeks. HE staining (a), SO/FG staining (b), immunohistochemical staining of COLII (c) and MMP13 (d), immunofluorescence staining of CD86 (e) and CD206 (f) at the synovial, and immunofluorescence staining of IL-1β (g) in the TMJ of rats after 8 weeks of treatment, scale bar = 100 μm. (h) Histological OARSI score of articular cartilage after 8 weeks of treatment and quantitative data of immunohistochemical positive area and proportion of immunofluorescence positive cells in (b–g). Data represent means ± SD, n = 3. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 indicates a significant difference in comparison to the Sham group. # p < 0.05, ## p < 0.01, and ### p < 0.001 suggests a significant difference in comparison to the MIA group.

    Article Snippet: Additionally, the sections were stained with antibodies specific to anti-CD86, CD206, and IL-1β (Boster Biological Technology Co., Ltd., Wuhan, China) for immunofluorescence analysis.

    Techniques: In Vivo, Staining, Immunohistochemical staining, Immunofluorescence, Comparison

    (A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

    Journal: bioRxiv

    Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression

    doi: 10.64898/2026.05.16.725632

    Figure Lengend Snippet: (A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

    Article Snippet: An acellular intact-spleen perfusion platform enables serial cellular, transcriptomic, proteomic, and functional pharmacodynamic sampling that identifies steroid-responsive transcriptional programs, validates SOCS3 protein induction, and nominates a CLC/Galectin-10-like non-transcriptional immunosuppressive axis for translation to discarded human donor spleens.

    Techniques: Quantitative Proteomics

    Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.

    Journal: bioRxiv

    Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression

    doi: 10.64898/2026.05.16.725632

    Figure Lengend Snippet: Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.

    Article Snippet: An acellular intact-spleen perfusion platform enables serial cellular, transcriptomic, proteomic, and functional pharmacodynamic sampling that identifies steroid-responsive transcriptional programs, validates SOCS3 protein induction, and nominates a CLC/Galectin-10-like non-transcriptional immunosuppressive axis for translation to discarded human donor spleens.

    Techniques: Recombinant, Staining, Flow Cytometry, Comparison, Control, Produced

    (A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

    Journal: bioRxiv

    Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression

    doi: 10.64898/2026.05.16.725632

    Figure Lengend Snippet: (A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

    Article Snippet: The assay was designed as an orthogonal functional test of the human immune counterpart of the porcine LGALS13-annotated / CLC-Galectin-10-like proteomic signal.

    Techniques: Quantitative Proteomics

    Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.

    Journal: bioRxiv

    Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression

    doi: 10.64898/2026.05.16.725632

    Figure Lengend Snippet: Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.

    Article Snippet: The assay was designed as an orthogonal functional test of the human immune counterpart of the porcine LGALS13-annotated / CLC-Galectin-10-like proteomic signal.

    Techniques: Recombinant, Staining, Flow Cytometry, Comparison, Control, Produced

    (A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

    Journal: bioRxiv

    Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression

    doi: 10.64898/2026.05.16.725632

    Figure Lengend Snippet: (A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

    Article Snippet: In parallel, data independent acquisition (DIA) proteomics of effluent cell pellets nominated a non-transcriptional protein-level response: a Sus scrofa LGALS13-annotated, CLC/Galectin-10-like galectin detected despite absence of the corresponding effluent-cell transcript.

    Techniques: Quantitative Proteomics

    Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.

    Journal: bioRxiv

    Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression

    doi: 10.64898/2026.05.16.725632

    Figure Lengend Snippet: Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.

    Article Snippet: In parallel, data independent acquisition (DIA) proteomics of effluent cell pellets nominated a non-transcriptional protein-level response: a Sus scrofa LGALS13-annotated, CLC/Galectin-10-like galectin detected despite absence of the corresponding effluent-cell transcript.

    Techniques: Recombinant, Staining, Flow Cytometry, Comparison, Control, Produced