rabbit anti clc2 (Alomone Labs)
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Rabbit Anti Clc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clc/Anti-CLC-2+(CLCN2)+Antibody/pm42230621-288-26-28
Average 92 stars, based on 14 article reviews
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Staining:Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 h, and then incubated for 48–72 h with Article Title: Gluconate-based compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels. Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1M PBS) for 2 h, and then incubated for 48–72 h with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Blocking Assay:Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 h, and then incubated for 48–72 h with Article Title: Gluconate-based compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels. Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1M PBS) for 2 h, and then incubated for 48–72 h with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Incubation:Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 h, and then incubated for 48–72 h with Article Title: Gluconate-based compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells. Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex. Article Title: Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels. Article Snippet: .. For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1M PBS) for 2 h, and then incubated for 48–72 h with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Severe changes in colon epithelium in the Mecp2 -null mouse model of Rett syndrome Article Snippet: .. Briefly, 4 mM paraffin sections were incubated with the corresponding primary antibody: Immunostaining:Article Title: Gluconate-based compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Glucose oxidase compositions as a neonate anticonvulsant Article Snippet: .. One group of slices was incubated in normal aCSF for 1 hr at 33° C. Another group of slices were incubated in 0 Mg2+ aCSF for 1 hr at 33° C. Slices were then fixed by 4% PFA overnight at 4° C. Immunostaining For CLC-3 staining, slices were pretreated with blocking solution (0.3% Triton-X and 5% normal donkey and goat serum in 0.1 M PBS) for 2 hr, and then incubated for 72 hr with Article Title: Poster Session Abstracts Article Snippet: .. The expression and tissue distribution of ClC-2 in mouse intestine was monitored by immunostaining using a C-terminal Purification:Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells. Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex. Centrifugation:Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells. Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex. Western Blot:Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells. Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex. SDS Page:Article Title: The interaction between megalin and ClC-5 is scaffolded by the Na⁺-H⁺ exchanger regulatory factor 2 (NHERF2) in proximal tubule cells. Article Snippet: Albumin endocytosis in the proximal tubule is mediated by a number of proteins, including the scavenger receptor megalin/cubilin and the PSD-95/Dlg/ZO-1 (PDZ) scaffolds NHERF1 and NHERF2.. In addition, in a number of in vitro and in vivo models, the loss of ClC-5 results in a decreased cell surface expression and whole cell level of megalin, suggesting an interaction between these two proteins in vivo.. We investigated if ClC-5 and megalin interact directly, and as ClC-5 binds to NHERF2, we investigated if this PDZ scaffold was required for a megalin/ClC-5 complex. Expressing:Article Title: Poster Session Abstracts Article Snippet: .. The expression and tissue distribution of ClC-2 in mouse intestine was monitored by immunostaining using a C-terminal |

