hek293t cl 0005 cells (Procell Inc)
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Procell Inc
hek293t cl 0005 cells
Hek293t Cl 0005 Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cl-0005/cells+hek293t/pm42106354-279-0-10
Average 86 stars, based on 1 article reviews
Hek293t Cl 0005 Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cl-0005/cells+hek293t/pm42106354-279-0-10
Average 86 stars, based on 1 article reviews
hek293t cl 0005 cells - by Bioz Stars,
2026-09
86/100 stars
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Recombinant:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Modification:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Magnetic Beads:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Protease Inhibitor:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: CCK-8 Assay:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: In Situ:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Isolation:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Proximity Ligation Assay:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Expressing:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Mutagenesis:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Purification:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: Mass Spectrometry:Article Title: HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Article Snippet: In brief Peng et al. demonstrate that TREM2 foamy macrophages undergo ferroptosis due to iron accumulation in advanced atherosclerosis, where the upregulated HMOX1-LDHB interaction facilitates LONP1-mediated TFAM degradation, causing mitochondrial dysfunction.. Treatment with MitoTEMPO or bortezomib, controlling mitochondrial homeostasis in foamy macrophages, suggests a potential therapeutic approach to alleviating atherosclerosis. Article Title: Revisiting phosphoregulation of Cdc25C during M-phase induction Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Plk1 inhibitor BI2536 Selleck Chemicals Cat# S1109 MEK1 inhibitor U0126 Calibiochem Cat# 109511-58-2 U0124 (inactive analog of U0126) Calibiochem Cat# 1140528-25-1 pan kinase inhibitor staurosporine MilliporeSigma Cat# S4400 glutathione resin GenScript Cat# L00206 reduced glutathione MilliporeSigma Cat# 3541 protein A/G agarose ThermoFisher Scientific Cat# 20241 Q-sepharose Fast Flow Pharmacia Cat# 17-0510-01 ATP-g-S Roche Cat# 11162306001 ATP-g-32P Perkin Elmer Cat# BLU003H lamda protein phosphatase New England Biolabs Cat# P0753 leupeptin Roche Cat# 11017101001 chymostatin Roche Cat# 11004638001 pepstatin Roche Cat# 11359053001 protease inhibitor cocktail Roche Cat# 11697498001 Dulbecco’s Modified Eagle’s medium Gibco Cat# C11995500BT L-glutamine Solarbio Life Sciences Cat# G8230 penicillin and streptomycin Solarbio Life Sciences Cat# P1400 thymidine Solarbio Life Sciences Cat# T8080 nocodazole Sigma-Aldrich Cat# M1404 Ponceau S solution Sigma-Aldrich Cat# P7170 Coomassie Brilliant blue Sigma-Aldrich Cat# 6104-59-2 Critical commercial assays PrimeSTAR GXL DNA Polymerase Takara Cat# R050A TnT Quick Coupled Transcription/Translation System Promega Corporation Cat# L2080 mMACHINE high yield capped RNA transcription kit Promega Corporation Cat# AM1340 Fast Mutagenesis System TransGen Biotech Cat# FM1-11 pMALTM Protein Fusion and Purification System New England Biolabs Cat# E8000S Bac-to-Bac Baculovirus Expression System (Version D) Cat# E8000S Cat# E8000S QIAexpress System QIAGEN Cat# 32149 Deposited data Unprocessed datasets from mass spectrometry https://peptideatlas.org/passel/ PASS05898 Experimental models: |
