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Detection of ferroptosis‐related pathway in chemoresistant NSCLC cells. (A) Detection of IC 50 in A549 and <t>A549/DDP</t> cell lines. (B) Western blot analysis of the expression levels of SLC7A11, GPX4, and FTH1, in A549 and A549/DDP. (C) qRT‐PCR for SLC7A11, GPX4, and FTH1 mRNA levels in A549 and A549/DDP. (D) Flow cytometry analysis of Lipid ROS levels in A549 cells and A549/DDP. (E) Verification of Lipid ROS fluorescence intensity in A549 and A549/DDP. (F) Detection of GSH levels in A549 and A549/DDP. (G) Detection of MDA levels in A549 and A549/DDP. (H) CCK‐8 assay for the sensitivity of A549/DDP cells to <t>cisplatin</t> after Erastin treatment. (ns, no significant, * p < 0.05, ** p < 0.01, *** p < 0.001).
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Detection of ferroptosis‐related pathway in chemoresistant NSCLC cells. (A) Detection of IC 50 in A549 and A549/DDP cell lines. (B) Western blot analysis of the expression levels of SLC7A11, GPX4, and FTH1, in A549 and A549/DDP. (C) qRT‐PCR for SLC7A11, GPX4, and FTH1 mRNA levels in A549 and A549/DDP. (D) Flow cytometry analysis of Lipid ROS levels in A549 cells and A549/DDP. (E) Verification of Lipid ROS fluorescence intensity in A549 and A549/DDP. (F) Detection of GSH levels in A549 and A549/DDP. (G) Detection of MDA levels in A549 and A549/DDP. (H) CCK‐8 assay for the sensitivity of A549/DDP cells to cisplatin after Erastin treatment. (ns, no significant, * p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: The FASEB Journal

Article Title: PCK2 Inhibition Reverses Cisplatin Resistance of Non‐Small Cell Lung Cancer by Triggering Ferroptosis

doi: 10.1096/fj.202602464R

Figure Lengend Snippet: Detection of ferroptosis‐related pathway in chemoresistant NSCLC cells. (A) Detection of IC 50 in A549 and A549/DDP cell lines. (B) Western blot analysis of the expression levels of SLC7A11, GPX4, and FTH1, in A549 and A549/DDP. (C) qRT‐PCR for SLC7A11, GPX4, and FTH1 mRNA levels in A549 and A549/DDP. (D) Flow cytometry analysis of Lipid ROS levels in A549 cells and A549/DDP. (E) Verification of Lipid ROS fluorescence intensity in A549 and A549/DDP. (F) Detection of GSH levels in A549 and A549/DDP. (G) Detection of MDA levels in A549 and A549/DDP. (H) CCK‐8 assay for the sensitivity of A549/DDP cells to cisplatin after Erastin treatment. (ns, no significant, * p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Cisplatin (DDP), Necrostatin‐1, Z‐VAD‐FMK, Ferrostatin‐1, and Chloroquine were purchased from MedChemexpress (USA).

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Flow Cytometry, Fluorescence, CCK-8 Assay