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red chromagen aec  (Vector Laboratories)


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    Structured Review

    Vector Laboratories red chromagen aec
    Penetration of panitumumab into tumor xenograft tissue is dose and time dependent. Panitumumab initially surrounded the afferent blood supply and then penetrated diffusely into the surrounding tumor tissue. A431 tumor xenograft samples of animals receiving 0, 20, 200, or 500 μg panitumumab were collected (A) 24 hours or (B) 96 hours after initiation of dosing and incubated with anti-idiotype IgG 2 -bound panitumumab to detect administered panitumumab (blue <t>chromagen)</t> and DAKO anti-EGFR to detect EGFR (red chromagen). Micron bars = 10 μm all high magnification images and black arrows identify blood vessels. (C) IHC staining intensities for the anti-idiotype IgG 2 -bound panitumumab were qualitatively graded and plotted as an estimated percent of total staining intensity.
    Red Chromagen Aec, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chromagen+aec/pmc03499177-61-1-5
    Average 86 stars, based on 1 article reviews
    red chromagen aec - by Bioz Stars, 2026-11
    86/100 stars

    Images

    1) Product Images from "Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer"

    Article Title: Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-11-47

    Penetration of panitumumab into tumor xenograft tissue is dose and time dependent. Panitumumab initially surrounded the afferent blood supply and then penetrated diffusely into the surrounding tumor tissue. A431 tumor xenograft samples of animals receiving 0, 20, 200, or 500 μg panitumumab were collected (A) 24 hours or (B) 96 hours after initiation of dosing and incubated with anti-idiotype IgG 2 -bound panitumumab to detect administered panitumumab (blue chromagen) and DAKO anti-EGFR to detect EGFR (red chromagen). Micron bars = 10 μm all high magnification images and black arrows identify blood vessels. (C) IHC staining intensities for the anti-idiotype IgG 2 -bound panitumumab were qualitatively graded and plotted as an estimated percent of total staining intensity.
    Figure Legend Snippet: Penetration of panitumumab into tumor xenograft tissue is dose and time dependent. Panitumumab initially surrounded the afferent blood supply and then penetrated diffusely into the surrounding tumor tissue. A431 tumor xenograft samples of animals receiving 0, 20, 200, or 500 μg panitumumab were collected (A) 24 hours or (B) 96 hours after initiation of dosing and incubated with anti-idiotype IgG 2 -bound panitumumab to detect administered panitumumab (blue chromagen) and DAKO anti-EGFR to detect EGFR (red chromagen). Micron bars = 10 μm all high magnification images and black arrows identify blood vessels. (C) IHC staining intensities for the anti-idiotype IgG 2 -bound panitumumab were qualitatively graded and plotted as an estimated percent of total staining intensity.

    Techniques Used: Incubation, Immunohistochemistry, Staining

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    Article Snippet: Color was developed using Chromagen AEC (SK 4200, Vector laboratories) and nuclei were counterstained with Mayer Hematoxylin (Sigma-Aldrich).



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    Penetration of panitumumab into tumor xenograft tissue is dose and time dependent. Panitumumab initially surrounded the afferent blood supply and then penetrated diffusely into the surrounding tumor tissue. A431 tumor xenograft samples of animals receiving 0, 20, 200, or 500 μg panitumumab were collected (A) 24 hours or (B) 96 hours after initiation of dosing and incubated with anti-idiotype IgG 2 -bound panitumumab to detect administered panitumumab (blue <t>chromagen)</t> and DAKO anti-EGFR to detect EGFR (red chromagen). Micron bars = 10 μm all high magnification images and black arrows identify blood vessels. (C) IHC staining intensities for the anti-idiotype IgG 2 -bound panitumumab were qualitatively graded and plotted as an estimated percent of total staining intensity.
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    Image Search Results


    Penetration of panitumumab into tumor xenograft tissue is dose and time dependent. Panitumumab initially surrounded the afferent blood supply and then penetrated diffusely into the surrounding tumor tissue. A431 tumor xenograft samples of animals receiving 0, 20, 200, or 500 μg panitumumab were collected (A) 24 hours or (B) 96 hours after initiation of dosing and incubated with anti-idiotype IgG 2 -bound panitumumab to detect administered panitumumab (blue chromagen) and DAKO anti-EGFR to detect EGFR (red chromagen). Micron bars = 10 μm all high magnification images and black arrows identify blood vessels. (C) IHC staining intensities for the anti-idiotype IgG 2 -bound panitumumab were qualitatively graded and plotted as an estimated percent of total staining intensity.

    Journal: Molecular Cancer

    Article Title: Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer

    doi: 10.1186/1476-4598-11-47

    Figure Lengend Snippet: Penetration of panitumumab into tumor xenograft tissue is dose and time dependent. Panitumumab initially surrounded the afferent blood supply and then penetrated diffusely into the surrounding tumor tissue. A431 tumor xenograft samples of animals receiving 0, 20, 200, or 500 μg panitumumab were collected (A) 24 hours or (B) 96 hours after initiation of dosing and incubated with anti-idiotype IgG 2 -bound panitumumab to detect administered panitumumab (blue chromagen) and DAKO anti-EGFR to detect EGFR (red chromagen). Micron bars = 10 μm all high magnification images and black arrows identify blood vessels. (C) IHC staining intensities for the anti-idiotype IgG 2 -bound panitumumab were qualitatively graded and plotted as an estimated percent of total staining intensity.

    Article Snippet: The red chromagen AEC (3-amino-9-ethylcarbazole; Vector Labs, Burlingame, CA) was used to visualize EGFR staining.

    Techniques: Incubation, Immunohistochemistry, Staining