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chemidoc mp multimode imager  (Bio-Rad)


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    Structured Review

    Bio-Rad chemidoc mp multimode imager
    Chemidoc Mp Multimode Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chemidoc+mp+multimode+imager/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/pmc13621846-284-6-10
    Average 99 stars, based on 2450 article reviews
    chemidoc mp multimode imager - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: Rapid, efficient auxin-inducible protein degradation in Candida pathogens
    Article Snippet: Proteins were separated on 10% tris-glycine SDS-PAGE gels, transferred to 0.45 μm nitrocellulose membranes (Bio-Rad) and probed overnight at 4°C with mouse anti-HA (1:5,000; Sigma-Aldrich, 12CA5), rabbit anti-V5 (1:5,000; Invitrogen, MA5-15253), mouse anti-c-Myc (1:2,000-1:5,000; Sigma-Aldrich, 9E10), or rabbit anti-PSTAIR (1:5,000; Millipore-Sigma, 06-923).

    Western Blot:

    Article Title: Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens
    Article Snippet: Immunoblots using HRP were developed with Clarity Western ECL Substrate (Bio-Rad, 170-5060). .. All immunoblots were imaged on a ChemiDoc MP multimode imager (Bio-Rad). .. Band intensities were quantified using ImageLab software (Bio-Rad).

    Article Title: Cdc14 phosphatase contributes to cell wall integrity and pathogenesis in Candida albicans
    Article Snippet: Secondary anti-mouse and anti-rabbit antibodies conjugated to horseradish peroxidase were from Jackson ImmunoResearch (115–035-003 or 111–035-003) and used at 1:10,000 dilution for 60 min at 4°C. .. Immunoblots were developed using Clarity Western ECL Substrate (Bio-Rad) and imaged on a ChemiDoc MP multimode imager (Bio-Rad). ..

    Article Title: Reduced Cdc14 phosphatase activity impairs septation, hyphal differentiation and pathogenesis and causes echinocandin hypersensitivity in Candida albicans
    Article Snippet: Secondary anti-mouse and anti-rabbit antibodies conjugated to horseradish peroxidase were from Jackson ImmunoResearch (115-035-003 or 111-035-003) and used at 1:10,000 dilution for 60 min at 4 °C. .. Immunoblots were developed using Clarity Western ECL Substrate (Bio-Rad, 170-5060) and imaged on a ChemiDoc MP multimode imager (Bio-Rad). ..

    Article Title: Rapid, efficient auxin-inducible protein degradation in Candida pathogens
    Article Snippet: Secondary anti-mouse and anti-rabbit antibodies conjugated to horseradish peroxidase were from Jackson ImmunoResearch (115–035-003 or 111–035-003) and used at 1:10,000 dilution for 60 min. at 4°C. .. Immunoblots were developed with Clarity Western ECL Substrate (Bio-Rad, 170–5060) and imaged on a ChemiDoc MP multimode imager (Bio-Rad). ..

    Article Title: Rapid, efficient auxin-inducible protein degradation in Candida pathogens
    Article Snippet: Secondary anti-mouse and anti-rabbit antibodies conjugated to horseradish peroxidase were from Jackson ImmunoResearch (115-035-003 or 111-035-003) and used at 1:10,000 dilution for 60 min at 4°C. .. Immunoblots were developed with Clarity Western ECL Substrate (Bio-Rad, 170-5060) and imaged on a ChemiDoc MP multimode imager (Bio-Rad). ..

    Article Title: Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens
    Article Snippet: Secondary anti-mouse and anti-rabbit antibodies conjugated to horseradish peroxidase were from Jackson ImmunoResearch (1:10,000; 115-035-003 or 111-035-003) and anti-rabbit IgG StarBright Blue 700 was from Bio-Rad (1:10,000; 12004161). .. Immunoblots were developed with Clarity Western ECL Substrate (Bio-Rad, 170-5060) and imaged on a ChemiDoc MP multimode imager (Bio-Rad). .. Band intensities were quantified using ImageLab software (Bio-Rad).



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    Bio-Rad bio rad chemidoc mp imaging system at multimode
    Fig. 1. A chromosomal tandem fluorescence reporter system allows facile monitoring of endogenous sRNA activity in E. coli. Schematic of the chiP (A) or sodB (D) dual-fluorescence reporter. Colony fluorescence on LB agar for reporter strains carrying translational fusions to chiP (B, WT [JC1200]; ΔchiX [JC1244]) or sodB (E, WT [JC1246]; Δfur [JC1248]; ΔfurΔryhB [JC1252]; and ΔfurΔhfq [JC1269]) was imaged using the Bio-Rad <t>ChemiDoc</t> MP Imaging System. Bright field imaging (B.F.) was used to visualize all colonies on the plate. chiP (C) or sodB (F) reporter fluorescence from cultures grown in LB for 6 h was measured using the TECAN Spark 10M microplate reader. Representative images for colony fluorescence are shown. For fluorescence quantification, three to six individual clones of each strain were grown and measured in 96-well microplates; data are plotted as mean and SD. A detailed description of fluorescence measurement and quantification is included in Materials and Methods.
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    Image Search Results


    Fig. 1. A chromosomal tandem fluorescence reporter system allows facile monitoring of endogenous sRNA activity in E. coli. Schematic of the chiP (A) or sodB (D) dual-fluorescence reporter. Colony fluorescence on LB agar for reporter strains carrying translational fusions to chiP (B, WT [JC1200]; ΔchiX [JC1244]) or sodB (E, WT [JC1246]; Δfur [JC1248]; ΔfurΔryhB [JC1252]; and ΔfurΔhfq [JC1269]) was imaged using the Bio-Rad ChemiDoc MP Imaging System. Bright field imaging (B.F.) was used to visualize all colonies on the plate. chiP (C) or sodB (F) reporter fluorescence from cultures grown in LB for 6 h was measured using the TECAN Spark 10M microplate reader. Representative images for colony fluorescence are shown. For fluorescence quantification, three to six individual clones of each strain were grown and measured in 96-well microplates; data are plotted as mean and SD. A detailed description of fluorescence measurement and quantification is included in Materials and Methods.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A fluorescence-based genetic screen reveals diverse mechanisms silencing small RNA signaling in E. coli .

    doi: 10.1073/pnas.2106964118

    Figure Lengend Snippet: Fig. 1. A chromosomal tandem fluorescence reporter system allows facile monitoring of endogenous sRNA activity in E. coli. Schematic of the chiP (A) or sodB (D) dual-fluorescence reporter. Colony fluorescence on LB agar for reporter strains carrying translational fusions to chiP (B, WT [JC1200]; ΔchiX [JC1244]) or sodB (E, WT [JC1246]; Δfur [JC1248]; ΔfurΔryhB [JC1252]; and ΔfurΔhfq [JC1269]) was imaged using the Bio-Rad ChemiDoc MP Imaging System. Bright field imaging (B.F.) was used to visualize all colonies on the plate. chiP (C) or sodB (F) reporter fluorescence from cultures grown in LB for 6 h was measured using the TECAN Spark 10M microplate reader. Representative images for colony fluorescence are shown. For fluorescence quantification, three to six individual clones of each strain were grown and measured in 96-well microplates; data are plotted as mean and SD. A detailed description of fluorescence measurement and quantification is included in Materials and Methods.

    Article Snippet: To visualize colony fluorescence, E. coli cells were streaked on LB agar supplemented with appropriate antibiotics and grown at 37 °C for 16 h. The next day, colonies on agar were directly imaged using the Bio-Rad ChemiDoc MP Imaging system at multimode (Cy2 for GFP, Cy3 for mCherry and Ponceau S for bright field, auto optimal exposure).

    Techniques: Fluorescence, Activity Assay, Imaging, Clone Assay