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tlr4  (InvivoGen)


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    InvivoGen tlr4
    Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, <t>TLR4,</t> or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly­(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).
    Tlr4, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cells+tlr4/HEK-Blue+hTLR4+cells/pmc13224164-370-9-20
    Average 97 stars, based on 520 article reviews
    tlr4 - by Bioz Stars, 2026-09
    97/100 stars

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    1) Product Images from "Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities"

    Article Title: Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities

    Journal: Journal of Medicinal Chemistry

    doi: 10.1021/acs.jmedchem.6c00372

    Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, TLR4, or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly­(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).
    Figure Legend Snippet: Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, TLR4, or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly­(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).

    Techniques Used: Control

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    Article Snippet: MA104 (ATCC: CRL-2378.1) and Caco-2 (ATCC: HTB37) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Moregate Biotech) and 100 μg/mL penicillin-streptomycin (Sigma). .. HEK-Blue cells (-null, -TLR2, -TLR4) were maintained in DMEM supplemented with heat-inactivated FBS, 100 mg/L Normocin (InvivoGen) with HEKBlue selection (InvivoGen) or Zeocin (InvivoGen). ..

    Article Title: A novel glycoform of the rotavirus outer capsid protein VP7 is secreted from polarized cells and activates innate immune cells.
    Article Snippet: MA104 (ATCC: CRL-2378.1) and Caco-2 (ATCC: HTB37) cells were cultured in Dulbecco’s modi fied Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Moregate Biotech) and 100 μg/mL penicillin-streptomycin (Sigma). .. HEK-Blue cells (-null, -TLR2, -TLR4) were maintained in DMEM supplemented with heat-inactivated FBS, 100 mg/L Normocin (InvivoGen) with HEKBlue selection (InvivoGen) or Zeocin (InvivoGen). ..



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    Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, TLR4, or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly­(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).

    Journal: Journal of Medicinal Chemistry

    Article Title: Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities

    doi: 10.1021/acs.jmedchem.6c00372

    Figure Lengend Snippet: Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, TLR4, or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly­(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).

    Article Snippet: HEK-Blue NOD2 (Cat. code: hkb-hnod2), TLR2 (Cat. code: hkb-htlr2), TLR4 (Cat. code: hkb-htlr4), and TLR7 (Cat. code: hkb-htlr7) cell lines (Invivogen, San Diego, CA) are derived from HEK293 cells by cotransfection of hNOD2, hTLR2, hTLR4, or hTLR7 genes, respectively, and a nuclear factor-κB (NF-κB)-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.

    Techniques: Control