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cellaca plx high-throughput image cytometer  (Revvity)


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    Structured Review

    Revvity cellaca plx high-throughput image cytometer
    Cellaca Plx High Throughput Image Cytometer, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellaca+plx/Cellaca+PLX+High-throughput+image+cytometer/custom%40plx-sys1%4010%2E64898%2F2026%2E05%2E11%2E724381
    Average 93 stars, based on 23 article reviews
    cellaca plx high-throughput image cytometer - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transmission Assay:

    Article Title: Cellaca® PLX image cytometer as an alternative for immunophenotyping, GFP/RFP transfection efficiencies, and apoptosis analysis.
    Article Snippet: Cell and gene therapy is a fast-growing field for cancer therapeutics requiring reliable instrumentation and technologies.. Key parameters essential for satisfying Chemistry Manufacturing and Controls criteria standards are routinely performed using flow cytometry.. Recently, image cytometry was developed for cell characterization and cell-based assays but had not yet demonstrated sufficient sensitivity for surface marker detection.

    Multiplex Assay:

    Article Title: A Multiplex Assay to Simultaneously Monitor Apoptosis and Necrosis Using the Cellaca® PLX Image Cytometer.
    Article Snippet: change in pH) or internal (i.e., oxidative stress, DNA damage, hypoxia) factors [1, 2].. External factors trigger the extrinsic pathway, commonly called the death receptor pathway and are responsible for initiating the apoptotic cascade [3].. The extrinsic pathway binds ligands to death receptors (DR) (members of the tumor necrosis factor (TNF) gene superfamily) on the cell surface [4].

    Apoptosis Assay:

    Article Title: A Multiplex Assay to Simultaneously Monitor Apoptosis and Necrosis Using the Cellaca® PLX Image Cytometer.
    Article Snippet: change in pH) or internal (i.e., oxidative stress, DNA damage, hypoxia) factors [1, 2].. External factors trigger the extrinsic pathway, commonly called the death receptor pathway and are responsible for initiating the apoptotic cascade [3].. The extrinsic pathway binds ligands to death receptors (DR) (members of the tumor necrosis factor (TNF) gene superfamily) on the cell surface [4].

    Cytometry:

    Article Title: A Multiplex Assay to Simultaneously Monitor Apoptosis and Necrosis Using the Cellaca® PLX Image Cytometer.
    Article Snippet: change in pH) or internal (i.e., oxidative stress, DNA damage, hypoxia) factors [1, 2].. External factors trigger the extrinsic pathway, commonly called the death receptor pathway and are responsible for initiating the apoptotic cascade [3].. The extrinsic pathway binds ligands to death receptors (DR) (members of the tumor necrosis factor (TNF) gene superfamily) on the cell surface [4].

    Article Title: A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer.
    Article Snippet: In cellular therapies chimeric antigen receptor (CAR) T or NK cells undergo phenotypic analysis at multiple stages during discovery and development of novel therapies.. Patient samples are routinely analyzed via flow cytometry for population identification and distribution of CD3, CD4, and CD8 positive T cells.. As an alternative or orthogonal method, image cytometry systems have been used to perform simple cell-based assays in lieu of flow cytometry.

    Marker:

    Article Title: A Multiplex Assay to Simultaneously Monitor Apoptosis and Necrosis Using the Cellaca® PLX Image Cytometer.
    Article Snippet: change in pH) or internal (i.e., oxidative stress, DNA damage, hypoxia) factors [1, 2].. External factors trigger the extrinsic pathway, commonly called the death receptor pathway and are responsible for initiating the apoptotic cascade [3].. The extrinsic pathway binds ligands to death receptors (DR) (members of the tumor necrosis factor (TNF) gene superfamily) on the cell surface [4].

    Cell Counting:

    Article Title: A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer.
    Article Snippet: In cellular therapies chimeric antigen receptor (CAR) T or NK cells undergo phenotypic analysis at multiple stages during discovery and development of novel therapies.. Patient samples are routinely analyzed via flow cytometry for population identification and distribution of CD3, CD4, and CD8 positive T cells.. As an alternative or orthogonal method, image cytometry systems have been used to perform simple cell-based assays in lieu of flow cytometry.

    Transfection:

    Article Title: A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer.
    Article Snippet: In cellular therapies chimeric antigen receptor (CAR) T or NK cells undergo phenotypic analysis at multiple stages during discovery and development of novel therapies.. Patient samples are routinely analyzed via flow cytometry for population identification and distribution of CD3, CD4, and CD8 positive T cells.. As an alternative or orthogonal method, image cytometry systems have been used to perform simple cell-based assays in lieu of flow cytometry.

    Concentration Assay:

    Article Title: A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer.
    Article Snippet: In cellular therapies chimeric antigen receptor (CAR) T or NK cells undergo phenotypic analysis at multiple stages during discovery and development of novel therapies.. Patient samples are routinely analyzed via flow cytometry for population identification and distribution of CD3, CD4, and CD8 positive T cells.. As an alternative or orthogonal method, image cytometry systems have been used to perform simple cell-based assays in lieu of flow cytometry.

    Staining:

    Article Title: A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer.
    Article Snippet: In cellular therapies chimeric antigen receptor (CAR) T or NK cells undergo phenotypic analysis at multiple stages during discovery and development of novel therapies.. Patient samples are routinely analyzed via flow cytometry for population identification and distribution of CD3, CD4, and CD8 positive T cells.. As an alternative or orthogonal method, image cytometry systems have been used to perform simple cell-based assays in lieu of flow cytometry.



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    ( A ) Overall survival of Npm1 cA , Ptpn11 E76K , and Npm1 cA / Ptpn11 E76K mice. ( B ) Representative picture of a spleen collected from a Npm1 cA / Ptpn11 E76K mouse at survival endpoint compared with age-matched Mx-Cre mouse spleen. Summary of spleen weights (in grams) of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( C ) White blood cell (WBC), red blood cell (RBC), and platelet (PLT) counts of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( D ) Representative peripheral blood flow <t>cytometry</t> plot (gated on CD45 + CD3 – CD19 – NK1.1 – cells; percentage based on total CD45 + cells) of Mx-Cre and Npm1 cA / Ptpn11 E76K mice 4 weeks after poly(I:C) induction (total n of 11 Mx-Cre mice and 14 Ptpn11 E76K / Npm1 cA mice). Data are presented as mean ± SD. Statistical analysis by 1-way ANOVA with Benjamini-Hochberg FDR correction applied. *FDR-adjusted P ≤ 0.05, ***FDR-adjusted P ≤ 0.001, ****FDR-adjusted P ≤ 0.0001.
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    Image Search Results


    ( A ) Overall survival of Npm1 cA , Ptpn11 E76K , and Npm1 cA / Ptpn11 E76K mice. ( B ) Representative picture of a spleen collected from a Npm1 cA / Ptpn11 E76K mouse at survival endpoint compared with age-matched Mx-Cre mouse spleen. Summary of spleen weights (in grams) of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( C ) White blood cell (WBC), red blood cell (RBC), and platelet (PLT) counts of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( D ) Representative peripheral blood flow cytometry plot (gated on CD45 + CD3 – CD19 – NK1.1 – cells; percentage based on total CD45 + cells) of Mx-Cre and Npm1 cA / Ptpn11 E76K mice 4 weeks after poly(I:C) induction (total n of 11 Mx-Cre mice and 14 Ptpn11 E76K / Npm1 cA mice). Data are presented as mean ± SD. Statistical analysis by 1-way ANOVA with Benjamini-Hochberg FDR correction applied. *FDR-adjusted P ≤ 0.05, ***FDR-adjusted P ≤ 0.001, ****FDR-adjusted P ≤ 0.0001.

    Journal: JCI Insight

    Article Title: Characterization of the clonal hierarchy and immunophenotype of PTPN11 mutations in acute myeloid leukemia

    doi: 10.1172/jci.insight.193779

    Figure Lengend Snippet: ( A ) Overall survival of Npm1 cA , Ptpn11 E76K , and Npm1 cA / Ptpn11 E76K mice. ( B ) Representative picture of a spleen collected from a Npm1 cA / Ptpn11 E76K mouse at survival endpoint compared with age-matched Mx-Cre mouse spleen. Summary of spleen weights (in grams) of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( C ) White blood cell (WBC), red blood cell (RBC), and platelet (PLT) counts of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( D ) Representative peripheral blood flow cytometry plot (gated on CD45 + CD3 – CD19 – NK1.1 – cells; percentage based on total CD45 + cells) of Mx-Cre and Npm1 cA / Ptpn11 E76K mice 4 weeks after poly(I:C) induction (total n of 11 Mx-Cre mice and 14 Ptpn11 E76K / Npm1 cA mice). Data are presented as mean ± SD. Statistical analysis by 1-way ANOVA with Benjamini-Hochberg FDR correction applied. *FDR-adjusted P ≤ 0.05, ***FDR-adjusted P ≤ 0.001, ****FDR-adjusted P ≤ 0.0001.

    Article Snippet: Cryopreserved samples were thawed and counted using the Cellaca PLX Image Cytometry System (Nexcelom).

    Techniques: Flow Cytometry