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magnetic cell separation kits  (Miltenyi Biotec)


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    Miltenyi Biotec magnetic cell separation kits
    Magnetic Cell Separation Kits, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+separation/Myeloid+Dendritic+Cell+Isolation+Kit%2C+human/pm42520804-271-13-17
    Average 94 stars, based on 37 article reviews
    magnetic cell separation kits - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Effector memory-type regulatory T cells display phenotypic and functional instability.
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. FACSorting and expansion of Treg cell subsets Freshly isolated PBMCs were enriched for CD4+ T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG- 56), and CD95 (DX2), all from BioLegend unless stated] for subsequent Treg cell subset isolation by FACSorting. ..

    Article Title: Effector memory–type regulatory T cells display phenotypic and functional instability
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. Freshly isolated PBMCs were enriched for CD4 + T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG-56), and CD95 (DX2), all from BioLegend unless stated] for subsequent T reg cell subset isolation by FACSorting. ..

    Article Title: Effector memory-type regulatory T cells display phenotypic and functional instability.
    Article Snippet: .. Freshly isolated PBMCs were enriched for CD4+ T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec). .. The following day, the cells were stained with monoclonal antibodies [CD3 (UCHT1) CD4 (OKT4), CCR7 (G043H7), CD25 (M- A251), and CD127 (A019D5), all from BioLegend unless stated, and CD45RA (2H4), from Beckman Coulter] for subsequent CD4+ TN cell FACsorting with on an FACSAria II (BD Biosciences) as Treg cell (CD127−CD25hi)–depleted CD3+CD4+CD45RA+CCR7+.

    Article Title: Effector memory–type regulatory T cells display phenotypic and functional instability
    Article Snippet: .. Freshly isolated PBMCs were enriched for CD4 + T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec). .. The following day, the cells were stained with monoclonal antibodies [CD3 (UCHT1) CD4 (OKT4), CCR7 (G043H7), CD25 (M-A251), and CD127 (A019D5), all from BioLegend unless stated, and CD45RA (2H4), from Beckman Coulter] for subsequent CD4 + T N cell FACsorting with on an FACSAria II (BD Biosciences) as T reg cell (CD127 − CD25 hi )–depleted CD3 + CD4 + CD45RA + CCR7 + .

    Selection:

    Article Title: Effector memory-type regulatory T cells display phenotypic and functional instability.
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. FACSorting and expansion of Treg cell subsets Freshly isolated PBMCs were enriched for CD4+ T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG- 56), and CD95 (DX2), all from BioLegend unless stated] for subsequent Treg cell subset isolation by FACSorting. ..

    Article Title: Effector memory–type regulatory T cells display phenotypic and functional instability
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. Freshly isolated PBMCs were enriched for CD4 + T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG-56), and CD95 (DX2), all from BioLegend unless stated] for subsequent T reg cell subset isolation by FACSorting. ..

    Article Title: Effector memory-type regulatory T cells display phenotypic and functional instability.
    Article Snippet: .. Freshly isolated PBMCs were enriched for CD4+ T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec). .. The following day, the cells were stained with monoclonal antibodies [CD3 (UCHT1) CD4 (OKT4), CCR7 (G043H7), CD25 (M- A251), and CD127 (A019D5), all from BioLegend unless stated, and CD45RA (2H4), from Beckman Coulter] for subsequent CD4+ TN cell FACsorting with on an FACSAria II (BD Biosciences) as Treg cell (CD127−CD25hi)–depleted CD3+CD4+CD45RA+CCR7+.

    Article Title: Effector memory–type regulatory T cells display phenotypic and functional instability
    Article Snippet: .. Freshly isolated PBMCs were enriched for CD4 + T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec). .. The following day, the cells were stained with monoclonal antibodies [CD3 (UCHT1) CD4 (OKT4), CCR7 (G043H7), CD25 (M-A251), and CD127 (A019D5), all from BioLegend unless stated, and CD45RA (2H4), from Beckman Coulter] for subsequent CD4 + T N cell FACsorting with on an FACSAria II (BD Biosciences) as T reg cell (CD127 − CD25 hi )–depleted CD3 + CD4 + CD45RA + CCR7 + .

    Staining:

    Article Title: Effector memory-type regulatory T cells display phenotypic and functional instability.
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. FACSorting and expansion of Treg cell subsets Freshly isolated PBMCs were enriched for CD4+ T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG- 56), and CD95 (DX2), all from BioLegend unless stated] for subsequent Treg cell subset isolation by FACSorting. ..

    Article Title: Effector memory–type regulatory T cells display phenotypic and functional instability
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. Freshly isolated PBMCs were enriched for CD4 + T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG-56), and CD95 (DX2), all from BioLegend unless stated] for subsequent T reg cell subset isolation by FACSorting. ..

    Bioprocessing:

    Article Title: Effector memory-type regulatory T cells display phenotypic and functional instability.
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. FACSorting and expansion of Treg cell subsets Freshly isolated PBMCs were enriched for CD4+ T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG- 56), and CD95 (DX2), all from BioLegend unless stated] for subsequent Treg cell subset isolation by FACSorting. ..

    Article Title: Effector memory–type regulatory T cells display phenotypic and functional instability
    Article Snippet: PBMCs were isolated using Biocoll (Biochrom) gradient centrifugation. .. Freshly isolated PBMCs were enriched for CD4 + T cells via positive selection by magnetically activated cell separation (CD4 MicroBeads, Miltenyi Biotec) and stained with monoclonal antibodies [CD4 (SK3) and CD25 (2A3), both from BD Biosciences; CD127 (R34.34), from Beckman Coulter; CD45RA (HI100), CCR7 (G043H7), CD45RO (UCHL1), CD62L (DREG-56), and CD95 (DX2), all from BioLegend unless stated] for subsequent T reg cell subset isolation by FACSorting. ..

    Suspension:

    Article Title: CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers
    Article Snippet: After centrifugation, NPC were collected from the interphase, resuspended in 5 ml MACS buffer (Miltenyi Biotec, Germany, cat. no. 130-117-336), and passed through a 30 μm nylon mesh (Miltenyi Biotec, Germany, cat. no. 130-098-458) to generate a single-cell suspension. .. The suspension was centrifuged for 10 minutes at 500 g. NPC were then sequentially separated using magnetic-activated cell separation (autoMACS Pro Separator, Miltenyi Biotec, Germany, cat. no. 130-092-545), starting with the least abundant cells (HSC), followed by KC and LSEC. ..

    Article Title: Alveolar epithelial type 2 cell specific loss of IGFBP2 activates inflammation in COVID-19.
    Article Snippet: .. The initial cell separation was performed by using CD45 magnetic beads (catalog # 130-045-801, Miltenyi Biotec) to isolate specific cell populations from the suspension. .. After filtering the cell mixture through various sized sieving filters (100-, 40-, 20 mm; Pluriselect, USA), suspension cells were treated with DNase and separated into different populations.

    Magnetic Beads:

    Article Title: Alveolar epithelial type 2 cell specific loss of IGFBP2 activates inflammation in COVID-19.
    Article Snippet: .. The initial cell separation was performed by using CD45 magnetic beads (catalog # 130-045-801, Miltenyi Biotec) to isolate specific cell populations from the suspension. .. After filtering the cell mixture through various sized sieving filters (100-, 40-, 20 mm; Pluriselect, USA), suspension cells were treated with DNase and separated into different populations.

    Article Title: Neutrophil trapping and nexocytosis, mast cell-mediated processes for inflammatory signal relay.
    Article Snippet: supplemented with 125 ng/mL rhSCF (PeproTech) and 20 ng/mL IL-3 (BioLegend). .. On day 35, cells were purified by positive cell separation using anti-human CD117 magnetic beads (Miltenyi-Biotec). .. Purity of PSCMCs was assessed by flow cytometry with anti-CD34-FITC (BioLegend), anti-CD45-APC (BioLegend), anti-CD117-PE-Cy7 (BioLegend) and anti-FcεRIa-BV421 (BioLegend).

    Purification:

    Article Title: Neutrophil trapping and nexocytosis, mast cell-mediated processes for inflammatory signal relay.
    Article Snippet: supplemented with 125 ng/mL rhSCF (PeproTech) and 20 ng/mL IL-3 (BioLegend). .. On day 35, cells were purified by positive cell separation using anti-human CD117 magnetic beads (Miltenyi-Biotec). .. Purity of PSCMCs was assessed by flow cytometry with anti-CD34-FITC (BioLegend), anti-CD45-APC (BioLegend), anti-CD117-PE-Cy7 (BioLegend) and anti-FcεRIa-BV421 (BioLegend).



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    Image Search Results


    Cell selection using automated magnetic cell sorting (A) Cells were stained with CD45-biotin and CD31-biotin and sorted using autoMACS® Pro Separator. (B) Number of cells before staining for autoMACS® separation (step 11), and number of cells recovered from positive selection (CD45 + and CD31 + cells) and negative selection (CD45 - and CD31 - cells) in step 23. Each dot represents combined numbers from inguinal, axillary and brachial lymph nodes from 6 mice. Colors indicate biological replicates. (C) Percentages of cells after separation compared to the pre-staining cell count performed in step 11. (D) Purity check of separated cells using flow cytometry and staining for CD45 and CD31. (E) Overlay plots of positive selection (blue) and negative selection (red). (F) Percentage of CD45 + , CD45 - and CD31 + cells recovered (∗∗∗∗ p value < 0.0001, ∗ p value < 0.05). (G) Final viability check of positive and negative selected cells acquired just before performing scRNA-sequencing analysis.

    Journal: STAR Protocols

    Article Title: Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seq

    doi: 10.1016/j.xpro.2026.104501

    Figure Lengend Snippet: Cell selection using automated magnetic cell sorting (A) Cells were stained with CD45-biotin and CD31-biotin and sorted using autoMACS® Pro Separator. (B) Number of cells before staining for autoMACS® separation (step 11), and number of cells recovered from positive selection (CD45 + and CD31 + cells) and negative selection (CD45 - and CD31 - cells) in step 23. Each dot represents combined numbers from inguinal, axillary and brachial lymph nodes from 6 mice. Colors indicate biological replicates. (C) Percentages of cells after separation compared to the pre-staining cell count performed in step 11. (D) Purity check of separated cells using flow cytometry and staining for CD45 and CD31. (E) Overlay plots of positive selection (blue) and negative selection (red). (F) Percentage of CD45 + , CD45 - and CD31 + cells recovered (∗∗∗∗ p value < 0.0001, ∗ p value < 0.05). (G) Final viability check of positive and negative selected cells acquired just before performing scRNA-sequencing analysis.

    Article Snippet: Timing: 125 min In this step, isolated lymph node cells are sorted using the Miltenyi autoMACS® cell separator.

    Techniques: Selection, FACS, Staining, Cell Characterization, Flow Cytometry, Sequencing