cell pellets (Miltenyi Biotec)

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Miltenyi Biotec cell pellets
Cell Pellets, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 410 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+pellets/Myelin+Removal+Beads+II%2C+human%2C+mouse/pm42420559-651-3-17
Average 96 stars, based on 410 article reviews

cell pellets - by Bioz Stars, 2026-09

96/100 stars

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Centrifugation:

Article Title: Coupling Adoptive Cell Therapy with Boron Neutron Capture Therapy: Using Functional Tumor-Infiltrating Lymphocytes for Tumor Delivery of Boron Carbide Nanoparticles.
Article Snippet: Nanomedicine offers promising strategies for targeted drug delivery, imaging, and molecular-level therapies.. However, the clinical translation of nanomedicine has often been hindered by the complex interactions of nanoparticles (NPs) with biological systems.. This study investigates a cell-based delivery platform designed to overcome some of these limitations, using clinical-grade tumor-infiltrating lymphocytes (TILs) as biological carriers of boron carbide (B4C) NPs in boron neutron capture therapy (BNCT).

Article Title: Coupling Adoptive Cell Therapy with Boron Neutron Capture Therapy: Using Functional Tumor-Infiltrating Lymphocytes for Tumor Delivery of Boron Carbide Nanoparticles
Article Snippet: .. Following a second centrifugation under the same conditions, cell pellets were resuspended in TexMACS medium (Miltenyi Biotec). ..

Extraction:

Article Title: Detecting Mitochondrial Free Radicals with Quantum Sensors: From Organelles to a D. melanogaster Model of Neurodegeneration
Article Snippet: .. Briefly, cell pellets were resuspended in mitochondrial extraction buffer (50 mM Tris-HCl pH 7.4, 100 mM KCl, 1.5 mM MgCl 2 , 1 mM EGTA, 50 mM HEPES, 100 mM sucrose; Sigma-Aldrich) and homogenized using a GentleMACS Dissociator (Miltenyi Biotec). ..

Red Blood Cell Lysis:

Article Title: The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis
Article Snippet: The digested cells were then filtered through the 70 μm Nylon cell strainers (Miltenyi Biote, 130-098-462), and centrifuged at 350 g for 7 min. .. Subsequently, the cell pellets were re-suspended in red blood cell lysis solution (Miltenyi Biote, 130-094-183) to remove the residual blood cells. .. Finally, dead cells were eliminated by a magnetic activated cell sorting kit (Miltenyi Biote, 130-090-101) according to the kit’s instructions.

Article Title: Orphan receptor GPR153 facilitates vascular damage responses by modulating cAMP levels, YAP/TAZ signaling, and NF-κB activation.
Article Snippet: .. For sorting of brain ECs by FACS, cell pellets were resuspended in 2ml red blood cell lysis buffer (130-094-183; Miltenyi) and incubate at RT for 2min. .. Following centrifugation at 500 x g for 5min, cells were washed with PBS and resuspended in FACS buffer and stained with antibodies against CD31 (558738; BD Biosciences) and CD45 (12-0451-82; Thermo Scientific) for 30min on ice.

Article Title: Orphan receptor GPR153 facilitates vascular damage responses by modulating cAMP levels, YAP/TAZ signaling, and NF-κB activation
Article Snippet: .. For sorting of brain ECs by FACS , cell pellets were resuspended in 2 ml red blood cell lysis buffer (130-094-183; Miltenyi) and incubate at RT for 2 min. .. Following centrifugation at 500 x g for 5 min, cells were washed with PBS and resuspended in FACS buffer and stained with antibodies against CD31 (558738; BD Biosciences) and CD45 (12-0451-82; Thermo Scientific) for 30 min on ice.

FACS:

Isolation:

Article Title: Microglial TDP-43 mediates myelin refinement and represses Tyrobp cryptic exon inclusion in mice.
Article Snippet: .. For myelin isolation, cell pellets were resuspended, supplemented with an appropriate volume of Myelin Removal Beads II (Miltenyi Biotec, 130-096-731) according to Miltenyi’s protocol. .. Samples were resuspended in 1ml and passed through an LS column (Miltenyi Biotec, 130-042-401) attached to a MACS Separator (Miltenyi Biotec, 130-091-051).

Staining:

Article Title: Myocardial disarray drives metabolic inefficiency in human cardiomyocytes
Article Snippet: hiPSC-CMs were dissociated into single cells with 0.05% trypsin-EDTA and stained with the fixable Zombie NIR TM Viability Kit (423105 BioLegend®) according to the manufacturer’s protocol. .. Then, collected cell pellets were fixed and permeabilized using Inside Stain kit (130-090-477 Miltenyi Biotech) for 10 min at room temperature. ..

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